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CAS:No.331-39-5

英文名称:Caffeic acid

纯度:HPLC≥98%

分子式:C9H8O4

分子量:180.15

储存条件:Store at RT，2 years.

咖啡酸
货号：
SC8010
品牌：
Solarbio
SKU 北京总部 北京海淀 武汉 广州
SC8010-20mg 咨询 咨询 咨询 咨询
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本品为分析标准品。

使用本产品的案例（仅供参考）

HPLC–MS

We used an HPLC system, which consisted of a binary solvent delivery system, an on-line degasser, an auto-sampler, a column temperature controller, and a diode array detector. Separation was conducted using a C18 column (5 µm, 150 mm × 4.6 mm), and the temperature was set to 40℃. The injection volume was 0.01 mL, and the flow rate was 1 mL/min. Gradient elution was conducted using a binary system consisting of 10 % formic acid in DW (A) and 10 % formic acid in acetonitrile (B). The utilized gradient was as follows: isocratic 1 % B for 6 min, 1% – 3% B for 4 min, 3 % – 15 % B for 20 min, 15 % – 40 % B for 20 min, 40 % – 90 % B for 5 min, 90 % B isocratic for 10 min, 90 % – 1 % B for 5 min, and 1 % B isocratic for 10 min. A re-equilibration operation followed. The UV detection wavelength was set to 280 nm.

The voltages of the sample cone and the capillary were set to 17 and 3000 V for the positive ion mode and 22 and 2000 V for the negative ion mode.

来源文献：[1]Zhang,Junhong,Tian,et al.Isolation and identification of phenolic compounds in Chinese purple yam and evaluation of antioxidant activity[J].LWT-Food Science &Technology, 2018, 96:161-165.


HPLC

HPLC was performed using an equipped with a DAD detector and a C18 (250 mm × 4.6 mm;5 µm). The mobile phase included 0.2% phosphoric acid plus 0.4% sodium 1-heptanesulfonate (A) and methanol (B) at a flow speed of 1.0 ml/min in the condition of a column temperature of 30℃. The detection wavelength was set at 214 nm. The reference standard sample injection volume was 10 µl. The gradient elution was as follows: 0–5 min (100–100% A, 0%–0% B), 5–60 min (100–60% A, 0%–40% B), 60–120 min (60–30% A, 40–70% B), 120–125 min (30–30% A, 70–70% B), 125–125.1 min (30–100% A, 70–0% B), 125.1–132 min (100–100% A, 0–0% B). The chromatographic data and peak area scores were collected and analyzed using ChemStation software.

来源文献：Duan Q, Liu T, Huang C, Shao Q, Ma Y, Wang W, Liu T, Sun J, Fang J, Huang G, Chen Z. The Chinese Herbal Prescription JieZe-1 Inhibits Membrane Fusion and the Toll-like Receptor Signaling Pathway in a Genital Herpes Mouse Model. Front Pharmacol. 2021 Sep 24;12:707695. doi: 10.3389/fphar.2021.707695. PMID: 34630083;PMCID: PMC8497740.


HPLC

HPLC conditions: chromatographic column was SB-C18 column (4.6 × 250 mm, 5 μm) and the column temperature was maintained at 30℃. The wavelength of ultraviolet detector were 280 nm and 320 nm, and the gradient elution with a flow rate at 0.8 mL/min was performed by mobile phase A (2% acetic acid in deionized water) and solvent B (methanol;0–10 min, 5–30%;10–35 min, 30–50%;35–40 min, 50–60%;40–45 min, 60–70%;45–50 min, 70–5%;50–55 min, 5%).

来源文献：Xu J, Qi Y, Zhang J, Liu M, Wei X, Fan M. Effect of reduced glutathione on the quality characteristics of apple wine during alcoholic fermentation. Food Chem. 2019 Dec 1;300:125130. doi: 10.1016/j.foodchem.2019.125130. Epub 2019 Jul 4. PMID: 31325746.


UHPLC

Chromatographic separation of SPLFA and SPLF was done on an Agilent 1290 UHPLC system  coupled to an auto sampler, a diode array detector (DAD) and an ACQUITY UHPLC® HSS T3 column (2.1 ×100 mm, 1.8 μm;Waters, Milford, MA, USA)maintained at 30 ℃. Eluent A was 0.05% formic acid in water and eluent B was 0.05% formic acid in acetonitrile. Elution of the SPLPA compounds was achieved using the following linear gradient elution (in %B): 0 min, 5%;20 min, 60%;20.1 min, 5%;30 min, 5%, and for the SPLF compounds: 0 min, 20%, 25 min, 50%, 25.1 min, 20%, 35 min, 20%. The injection volume was 5 μL, with a flow rate of 0.3 mL/ min. All sample solutions were filtered through a 0.22 μm PTFE filter (Agilent Technologies) before analysis. The detection wavelength was 320 nm for SPLPA and 254 nm for SPLF.

来源文献：[1] Luo D , Mu T , Sun H .Profiling of phenolic acids and flavonoids in sweet potato (Ipomoea batatas L.) leaves and evaluation of their anti-oxidant and hypoglycemic activities[J].Food Bioscience, 2020, 39(44):100801.DOI:10.1016/j.fbio.2020.100801.


HPLC

HPLC analysis employed an Agilent 1260 HPLC instrument and a diode array detector (Agilent, Santa Clara, CA, USA). The chromatographic separation was achieved by a Zobax Stablebond Analytical SB-C18 column (250 × 4.6 mm, 5 μm). The mobile phases, containing a gradient of solvent A (acetic/water, 2:98, v/v) and solvent B (acetic acid/acetonitrile/water, 5:50:45, v/v) were used to elute the column. The solvent gradient was programmed from 90% to 55%A and 10% to 45%B for 50 min, 55% to 0%A and 45% to 100%B for 10 min, and 0% to 90%A and 100% to 10%B for 5 min. The flow velocity was 1.0 mL·min-1 , the column temperature was 30℃, and the injection volume was 20 μL

来源文献：Liang D, Deng H, Deng Q, Lin L, Lv X, Wang J, Wang Z, Xiong B, Zhao X, Xia H. Dynamic Changes of Phenolic Compounds and Their Associated Gene Expression Profiles Occurring during Fruit Development and Ripening of the Donghong Kiwifruit. J Agric Food Chem. 2020 Oct 14;68(41):11421-11433. doi: 10.1021/acs.jafc.0c04438. Epub 2020 Sep 30. PMID: 32936614.


HPLC-DAD

The identification and quantification of phenolics in the S. miltiorrhiza leaves extract before and after enrichment were analyzed using an Agilent 1260 Infinity LC system (Agilent Co., Palo Alto, CA, USA).10 μL of sample dissolved in 80 % methanol was injected into the HPLC system, and phenolics were chromatographed over a YMC-Pack ODS-A (250 × 4.6 mm, 5 μm) column at room temperature with a combination of 0.1 % trifluoroacetic acid (A) and acetonitrile (B) at a flow rate of 1 mL/min. The procedure for elution was as follows: 0− 5 min, 10 % B;5− 10 min, 10 %–12 % B;10− 20 min, 12 %–15 % B;20− 25 min, 15 %–20 % B;25− 60 min, 20 %–30 % B;60− 65 min, 30 %–100 % B;65− 70 min, 100 % B;70− 80 min, 100 %-10 % B. Phenolics were detected by a diode array detector (DAD) at 280 nm.

来源文献：[1] Hou M , Hu W , Hao K ,et al.Enhancing the potential exploitation of Salvia miltiorrhiza Bunge: Extraction, enrichment and HPLC-DAD analysis of bioactive phenolics from its leaves[J].Industrial Crops and Products, 2020, 158:113019.DOI:10.1016/j.indcrop.2020.113019.


HPLC

A Sunfire C18 reverse phase chromatographic column (250 × 4.6 mm,5 µm) (Waters, Milford, MA, USA) was used with a flow rate of 1 mL/min and a column temperature of 30 ◦C. The mobile phases A and B consisted of 2% (v/v) acetic acid solution (A) and 2% (v/v) acetic acid-acetonitrile solution, respectively. Gradient elution was used with the following conditions: 0 min, 5% B; 40 min, 20% B; 55 min, 27.5% B; 65 min, 50% B; 66–71 min, 80% B; 73–78 min, 5% B. The injection volume was set to 10 µL, and all phenolic compounds were detected at 200–650 nm.

文献来源：Zhao Y, Liu D, Zhang J, et al. Improving Soluble Phenolic Profile and Antioxidant Activity of Grape Pomace Seeds through Fungal Solid-State Fermentation[J]. Foods, 2024, 13(8): 1158.

备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.

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