中文
英语
产品简介
| 中文名称 | 革兰氏染色试剂盒 |
| 英文名称 | Gram Stain Kit |
| 别名 | 革兰染液 革兰氏染色试剂盒 |
| 储存条件 | 2-8℃，避光保存，有效期1年。 |
| 规格 | 4*10ml 4*100ml 4*500ml |
| 单位 | 盒 |
革兰氏染色法是细菌学中广泛使用的一种常规菌种鉴别染色法。
其染色原理为通过结晶紫初染和碘液媒染后，在细胞壁内形成了不溶于水的结晶紫与碘的复合物，能被革兰氏阳性菌较厚的细胞壁保留在细胞膜上，使革兰氏阳性菌呈紫色。革兰氏阴性菌细胞壁薄、外膜层类脂含量高、肽聚糖层薄且交联度差，脱色后类脂外膜迅速溶解，缝隙加大，结晶紫与碘复合物溶出，因此能被乙醇脱色，后经番红复染呈红色。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
实验图
大肠杆菌/金黄葡萄球菌涂片
金黄葡萄球菌着紫色，大肠杆菌着红色
金黄葡萄球菌着紫色，大肠杆菌着红色
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Liu Guo-Hong, Narsing Rao Manik Prabhu, Chen Qian-Qian, Che Jian-Mei, Shi Huai, Liu Bo, Li Wen-Jun
IF：
3.0350
Publish_to：
EXTREMOPHILES
PMID：
35661272
Integration of Lysin into Chitosan Nanoparticles for Improving Bacterial Biofilm Inhibition
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Author：
Liu Bingxin, Li Zong, Guo Qiucui, Guo Xiaoxiao, Liu Ruyin, Liu Xinchun
IF：
3.0000
Publish_to：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
PMID：
37436548
Characterization of Marinilongibacter aquaticus gen. nov., sp. nov., a unique marine bacterium harboring four CRISPR-Cas systems in the phylum Bacteroidota
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Author：
Zhang Dao-Feng, Yao Yu-Fang, Xue Hua-Peng, Fu Zi-Yue, Zhang Xiao-Mei, Shao Zongze
IF：
2.9020
Publish_to：
JOURNAL OF MICROBIOLOGY
PMID：
35913596
Fungi on the cuticle surface increase the resistance of Aedes albopictus to deltamethrin
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Author：
Ju-Ping Hu, Si-Jia Deng, Lin Gu, Lin Li, Lei Tu, Ju-Lin Li, Jian-Xia Tang, Guo-Ding Zhu
IF：
2.9000
Publish_to：
Insect Science
PMID：
39828985
Comparative genomic analysis and proposal of Clostridium yunnanense sp. nov., Clostridium rhizosphaerae sp. nov., and Clostridium paridis sp. nov., three novel Clostridium sensu stricto endophytes with diverse capabilities of acetic acid and ethanol produ
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Author：
Cong-Jian Li, Zhen Zhang, Peng-Chao Zhan, Ai-Ping Lv, Pan-Pan Li, Lan Liu, Wen-Jun Li, Ling-Ling Yang, Xiao-Yang Zhi
IF：
2.8370
Publish_to：
ANAEROBE
PMID：
36535584
Shouchella tritolerans sp. nov., a facultative anaerobic bacterium isolated from marine sediments
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Author：
Xie Cheng-Jie, Yang Shang, Han Shuang, Liu Guo-Hong, Zhou Shun-Gui
IF：
2.8000
Publish_to：
ARCHIVES OF MICROBIOLOGY
PMID：
36961602
Chryseobacterium endalhagicum sp. nov., isolated from seed of leguminous plant
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Author：
Xiaobo Zhang, Xingyan Guo, Mayina Kahaer, Tingting Tian, Yuping Sun
IF：
2.7470
Publish_to：
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
PMID：
34739366
Pararoseburia lenta gen. nov., sp. nov. isolated from human faeces
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Author：
Rashidin Abdugheni, Yu-Jing Wang, Dan-Hua Li, Meng-Xuan Du, Chang Liu, Nan Zhou, Shuang-Jiang Liu
IF：
2.6890
Publish_to：
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
PMID：
35559790
Bacteroides rhinocerotis sp. nov.,isolated from the fresh feces of rhinoceros in Beijing Zoo
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Author：
Li Xue, Sun Peilin, Gong Liang, Shi Weixiong, Xiang Zhiguang, Li Ming, Su Lei, Qin Chuan
IF：
2.6670
Publish_to：
ARCHIVES OF MICROBIOLOGY
PMID：
37017778
Anti-Inflammatory and Antibacterial Potential of Qicao Rukang Powder in Bovine Subclinical Mastitis
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Author：
Bereket H. Imam, Ayodele O. Oladejo, Xiaohu Wu, Jie Yang, Xiaoyu Ma, Wenxiang Shen, Jiang Wei, Zuoting Yan, Xuezhi Ding
IF：
2.6300
Publish_to：
Evidence-based Complementary and Alternative Medicine
PMID：
34484387
Genome sequence and comparative analysis of fungal antagonistic strain Bacillus velezensis LJBV19
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Author：
Wang Bo, Yang Bohan, Peng Hang, Lu Jiang, Fu Peining
IF：
2.6290
Publish_to：
FOLIA MICROBIOLOGICA
PMID：
35913660
Clostridium lamae sp. nov., a novel bacterium isolated from the fresh feces of alpaca
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Author：
Li Xue, Li Ming, Shi Weixiong, Li Xia, Xiang Zhiguang, Su Lei
IF：
2.6000
Publish_to：
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
PMID：
38367205
Propioniciclava soli sp. nov., isolated from forest soil, Yunnan, China, and reclassification of the genus Brevilactibacter into the genus Propioniciclava, and Brevilactibacter sinopodophylli, Brevilactibacter flavus, and Brevilactibacter coleopterorum as
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Author：
Li Le-Le, Zhao Jiang-Yuan, Geng Yu, Zhou Yu-Guang, Yuan Hui-Ren, Cai Man, Wen Meng-Liang, Li Ming-Gang, Tang Shu-Kun
IF：
2.5520
Publish_to：
ARCHIVES OF MICROBIOLOGY
PMID：
34928401
Safety assessment of potential probiotic Lactobacillus acidophilus AM13-1 with high cholesterol-lowering capability isolated from human gut
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Author：
Liu Xudong, Wang Mengmeng, Hu Tongyuan, Lin Xiaoqian, Liang Hewei, Li Wenxi, Zhao Shaowei, Zhong Yiyi, Zhang Haifeng, Ge Lan, Jin Xin, Xiao Liang, Zou Yuanqiang
IF：
2.4000
Publish_to：
LETTERS IN APPLIED MICROBIOLOGY
PMID：
38126115
Sphingopyxis jiangsuensis sp. nov. Isolated From the Surface Water of the Yellow Sea
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Author：
Gao Zeng-Yu, Xue Hua-Peng, Wang Lu, Yao Yutian, Zhang Dao-Feng, Huang Jianke, Liu Chuang, Zhang Ai Hua
IF：
2.3430
Publish_to：
CURRENT MICROBIOLOGY
PMID：
35672580
Crenobacter oryzisoli sp. nov., a novel phosphate-solubilizing bacterium isolated from the paddy soil
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Author：
Zhang Shu-Qun, Xie Cheng-Jie, Yao Ling, Rensing Christopher, Lin Hao, Liu Guo-Hong, Zhou Shun-Gui
IF：
2.3000
Publish_to：
ARCHIVES OF MICROBIOLOGY
PMID：
38954015
Altererythrobacter flava sp. nov., a new member of the family Erythrobacteraceae, isolated from a surface seawater sample
点击查看 >>
Author：
Zhang Xiao-Mei, Zhang Dao-Feng, Zhang Yuan-Lan
IF：
2.2710
Publish_to：
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
PMID：
33661468
Revealing microbial diversity in buffalo milk with high somatic cell counts: implications for mastitis diagnosis and treatment
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Author：
Jiang Hancai, Xu Jiayin, Xu Xiaoxian, Wei Jue, Liu Jinfeng, Qin Chaobin, Miao Wenhao, Li Ling, Song Xinhui, Liu Qingyou, Cui Kuiqing, Li Zhipeng
IF：
2.2000
Publish_to：
VETERINARY RESEARCH COMMUNICATIONS
PMID：
38874832
Pseudomonas pratensis sp. nov., Isolated from Grassland Soil from Inner Mongolia, China
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Author：
Zhang Meng, Shen Lixin, Shi Jiajia, Chen Linxia, Luo Ying, Sun Jiquan, Fan Deliang, Hara Shintaro, Bao Zhihua
IF：
2.1880
Publish_to：
CURRENT MICROBIOLOGY
PMID：
33389060
Pseudomonas subflava sp. nov.,a new Gram-negative bacterium isolated from Guishan in Yunnan province,south–west China
点击查看 >>
Author：
Ling Cheng, Zhao Jiang-Yuan, Li Le-Le, Ding Zhang-Gui, Zhang Meng-Yu, Tang Jing, Liang Song-Guo, Li Jian-Yu, Liu Xiao-Di, Feng Lu-Yao, Yang Pei-Wen, Lu Yao, Shi Zhu-Feng, Kong Chui-Si, Li Ming-Gang, Tang Shu-Kun
IF：
2.1580
Publish_to：
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
PMID：
37097385
Pseudomonas beijingensis sp. nov., a novel species widely colonizing plant rhizosphere
点击查看 >>
Author：
Kaiji Liao, Jingyi Wu, Can Wang, Jun-Zhou Li, Hai-Lei Wei
IF：
2.0000
Publish_to：
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
PMID：
39058535
Thalassospira aquimaris sp. nov. and Winogradskyella marincola sp. nov. two marine bacteria isolated from an agar-degrading co-culture
点击查看 >>
Author：
Fu Zi-Yue, Zhang Dao-Feng, Huang Meng-Han, Wang Hong-Chuan, Chen Xiao-Ye, Yao Yu-Fang, Yuan Yang, Li Wen-Jun
IF：
1.8000
Publish_to：
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
PMID：
39008162
Genome Sequence of Acinetobacter baumannii Strain SHOU-Ab01, Isolated from Chinese Giant Salamander (Andrias davidianus) Liver
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Author：
Lu Yuan, Xiaoyan Zhang, Yufei Lyu, Yan Guo, Jie Chen, Dongshu Wang, Liping Wang, Hengliang Wang, Xiankai Liu
IF：
0.6000
Publish_to：
Microbiology Resource Announcements
PMID：
35862908
技术问答
（12）
注意：
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
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你好，G1060这个革兰染色试剂盒，里面的结晶紫浓度是多少？2025-04-24 10:11:46
您好，浓度大概在2%-3%之间，具体浓度不便告知
番红染液的番红染料浓度是多少？2024-10-18 13:37:15
您好，大约为0.50%
该试剂盒各个试剂的浓度是多少？2024-09-29 11:25:08
您好，涉及到配方，不方便透露具体浓度，革兰氏结晶紫浓度范围主要在1%-2.5%，番红浓度范围主要在1%-2%，
脱色试剂可以用95%酒精直接代替吗2024-08-15 10:07:04
您好，可以
这个和革兰氏染色快速法是一样的吗?2024-02-16 22:34:47
您好，该染色试剂盒为经典革兰氏染色试剂盒，无法保证使用快速法得到良好染色效果。