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L-酪氨酸

CAS:No.60-18-4

英文名称:L-Tyrosine

纯度:HPLC≥98%

分子式:C9H11NO3

分子量:181.19

储存条件:Store at RT,2 years.

L-酪氨酸
货号:
ST8360
品牌:
Solarbio
SKU 北京总部 北京海淀 武汉 广州
ST8360-200mg 咨询 咨询 咨询 咨询
数量:
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 本品为分析标准品。

使用本产品的案例(仅供参考

HPLC

The SA content was determined using high-performance liquid chromatography with a 4.6 × 250 mm column . The mobile phase had a flow rate of 0.7 mL/min (isocratic elution) and was composed of acetonitrile and 0.1% phosphoric acid (97:3, v/v). The column temperature was 25 ℃, a UV–visible detector was used for detection at a wavelength of 213 nm, and the injection volume was 20 μL. 

来源文献:Shi Y, Chen G, Chen K, Chen X, Hong Q, Kan J. Assessment of fresh star anise (Illicium verum Hook.f.) drying methods for influencing drying characteristics, color, flavor, volatile oil and shikimic acid. Food Chem. 2021 Apr 16;342:128359. doi: 10.1016/j.foodchem.2020.128359. Epub 2020 Oct 13. PMID: 33092919.


LC/MSn-IT-TOF

LC-MS/MS was used for the analysis and quantification of dopamine, phenylalanine, tyrosine, and L-dopa. LC separation was achieved using an C18 column (5 μ × 150 mm) maintained at 40℃. The mobile phase for L-dopa, dopamine, tyrosine, and phenylalanine consisted of water–formic acid (100:0.1, v/v) (A) and acetonitrile (B) with a linear gradient elution (A:B, 0 min, 90:10; 2 min, 90:10; 3 min, 5:95; 5 min, 5:95; 5.01 min, 90:10; 8 min 90:10) at a flow rate of 0.8 mL/min during the whole gradient cycle. Shimadzu LC-MS solution (Version 5.72) was used for data acquisition and processing. For positive ESI analysis, the parameters were as follows: nebulizer gas, 3 L/min; drying gas, 10.0 L/min; interface, −4.5 kV; CID gas, 230 kPa; and DL temperature, heat block temperature were maintained at 200 ℃ and 250 ℃, respectively. Quantification was carried out using multiple reaction monitoring mode. The m/z transitions were 154.20 → 91.10 (m/z) for dopamine, 198.15 → 107.05 (m/z) for L-dopa, 165.85 → 120.20 (m/z) for phenylalanine, 182.00 → 136.10 (m/z) for tyrosine, and 108.20 → 91.10 (m/z) for the IS. The peak areas of those compounds in fluid samples and the IS were recorded, respectively.

来源文献:Wang Y, Tong Q, Ma SR, Zhao ZX, Pan LB, Cong L, Han P, Peng R, Yu H, Lin Y, Gao TL, Shou JW, Li XY, Zhang XF, Zhang ZW, Fu J, Wen BY, Yu JB, Cao X, Jiang JD. Oral berberine improves brain dopa/dopamine levels to ameliorate Parkinson's disease by regulating gut microbiota. Signal Transduct Target Ther. 2021 Feb 24;6(1):77. doi: 10.1038/s41392-020-00456-5. PMID: 33623004; PMCID: PMC7902645.

备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.

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