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Cole苏木素染色液(常规染色)
英文名称:Cole’s Hematoxylin Solution(For Conventional Stain)
储存条件:RT保存，有效期1年。
产品简介
| 中文名称 | Cole苏木素染色液(常规染色) |
| 英文名称 | Cole’s Hematoxylin Solution(For Conventional Stain) |
| 别名 | 苏木精染色液 |
| 储存条件 | RT保存，有效期1年。 |
| 规格 | 100ml 500ml |
| 单位 | 瓶 |
苏木素是组织化学和免疫组织化学中最常用的染料之一，可以广泛用于组织切片或培养细胞的染色，也被称为苏木精染色。无色的苏木素氧化后形成有醌环结构的氧化苏木素(亦称为苏木精)，从而可以和三价的铝离子、铁离子等形成有颜色的带正电荷的复合物。细胞核内基因组DNA的双螺旋结构中，双链上的磷酸基团向外，带负电荷，呈酸性，很容易与带正电荷的氧化苏木素复合物结合，从而形成细胞核染色。苏木素染色液有多种不同的配制方法，不同的方法可以把细胞核染色成不同深浅的蓝色或蓝紫色。
本染色液采用改良经典配方，不含汞、甲醇等有毒有害试剂。本染色液属于进行性染色，染色快条件温和，无需酸性分化，可以和免疫荧光染色或免疫组化染色等配合使用。可以在苏木素染色液染色后进行免疫荧光染色或其它染料的染色，也可以在免疫组化染色后再进行苏木素复染。染色步骤简单，操作时间短。苏木素染色后细胞核呈现蓝色。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
实验图
小鼠肝脏石蜡切片
细胞核着蓝色
细胞核着蓝色
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Jinyan Zheng, Yongdong Dai, Xiang Lin, Qianmeng Huang, Libing Shi, Xiaoying Jin, Na Liu, Feng Zhou, Songying Zhang
IF：
2.9520
Publish_to：
Molecular Medicine Reports
PMID：
34278456
FOXF2 Regulates PRUNE2 Transcription in the Pathogenesis of Colorectal Cancer
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Author：
Ting Li, Silin Huang, Wei Yan, Yu Zhang, Qiang Guo
IF：
2.8760
Publish_to：
TECHNOLOGY IN CANCER RESEARCH & TREATMENT
PMID：
35929169
Effect of Wuzi Yanzong Pills on Sertoli cells and blood–testis barrier in heat-stressed rats based on Akt signalling pathway
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Author：
Ya-hui Xu, Yue Li, Su-qin Hu, Chun-rui Li, Dian-long Liu, Ke Hu, Li-dan Cui, Jian Guo
IF：
2.7750
Publish_to：
ANDROLOGIA
PMID：
34197007
Mutual growth-promoting effect between Bifidobacterium bifidum WBBI03 and Listeria monocytogenes CMCC 54001
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Author：
Dong Yang,Xiaoli Wu,Xiaomin Yu,Lihua He,Nagendra P
IF：
2.7490
Publish_to：
Journal of Dairy Science
PMID：
28259400
Tangshen Decoction Enhances Podocytes Autophagy to Relieve Diabetic Nephropathy through Modulation of p-AMPK/p-ULK1 Signaling
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Author：
Ling Yan, Xiaoxiao Xu, Yanbo Fan, Lifang Zhang, Xiaojing Niu, Aimin Hu
IF：
2.6500
Publish_to：
Evidence-based Complementary and Alternative Medicine
PMID：
35449816
Neuroprotective effect of allicin in a rat model of acute spinal cord injury
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Author：
R Lv, N Mao, J Wu, C Lu, M Ding, X Gu, Y Wu, Z Shi
IF：
2.5550
Publish_to：
Life sciences
PMID：
26546416
Alanyl-glutamine ameliorates lipopolysaccharide-induced inflammation and barrier function injury in bovine jejunum epithelial cells
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Author：
Xianglun Zhang, Xiuwen Tan, Yifan Liu, Wei You, Gu
IF：
2.2500
Publish_to：
Biochemistry and Cell Biology
PMID：
30773024
Nanodiamond-conjugated transferrin as chemotherapeutic drug delivery
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Author：
Zhiqin Wang,Zhimei Tian,Yu Dong,Lin Li,Lu Tian,Yin
IF：
2.2320
Publish_to：
Diamond and Related Materials
PMID：
24998271
The expression of ASAP3 and NOTCH3 and the clinicopathological characteristics of adult glioma patients
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Author：
Li-ping Su, Min Ji, Li Liu, Wei Sang, Jing Xue, Bo Wang, Hong-Wei Pu, Wei Zhang
IF：
2.1230
Publish_to：
Open Medicine
PMID：
36382054
Trophoblast glycoprotein promotes pancreatic ductal adenocarcinoma cell metastasis through Wnt/planar cell polarity signaling
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Author：
Ping He, Shuheng Jiang, Mingze Ma ,Yang Wang, Rong
IF：
1.9220
Publish_to：
Molecular Medicine Reports
PMID：
25738465
Inactivated Tianjin strain, a novel genotype of Sendai virus, induces apoptosis in HeLa, NCI-H446 and Hep3B cells
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Author：
Jun Chen, Han Han, Bin Wang, Liying Shi
IF：
1.6640
Publish_to：
Oncology
Letters
PMID：
27347098
Vitellogenin2: spermatozoon specificity and immunoprotection in mud crabs
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Author：
Ya nan Yang , Biqi Zheng , Chenchang Bao , Huiya
IF：
1.6470
Publish_to：
Reproduction
PMID：
27458256
技术问答
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这个染色液染细胞核一般染多长时间2025-03-20 21:30:03
您好，石蜡切片样本一般染色4-7min，冰冻切片样本一般染色1-3min，细胞样本一般染色3-5min
使用G1140苏木素染色后，需要用自来水反蓝吗，需要用盐酸酒精分色吗2024-12-16 20:39:30
您好，?Cole 苏木素染色液通常无需分化，但需通过返蓝调整核染色效果。?
染色完具体怎么反蓝呢2024-11-02 02:54:54
您好，可以用自来水反蓝10min或者1xPBS反蓝3-5min
您好，这个和HE试剂盒里的苏木素一样吗2024-09-23 09:31:15
您好，一样的
可以染细胞爬片吗？有染细胞的具体参考步骤吗？2024-09-06 09:15:02
您好，可以染色细胞爬片，首先需要用预冷的4%多聚甲醛固定下细胞爬片15min防止细胞脱落，然后轻柔去除固定液，使用纯水或蒸馏水清洗细胞2次，再进行苏木素染色3-5min（根据染色情况决定是否需要分化），最后用自来水返蓝10min，每步完成后均用蒸馏水水洗。