己糖激酶(HK)活性检测试剂盒
检测方法:微量法 Spectrophotometer/Microplate Reader
英文名称:Hexokinase(HK) Activity Assay Kit
储存条件:Store at -20℃,6 months


产品简介
中文名称 | 己糖激酶(HK)活性检测试剂盒 |
英文名称 | Hexokinase(HK) Activity Assay Kit |
别名 | 己糖激酶试剂盒 HK Kit 己糖激酶(HK)试剂盒 己糖激酶(HK)测试盒 |
检测方法 | 微量法 Spectrophotometer/Microplate Reader |
储存条件 | Store at -20℃,6 months |
推荐 | 若使用96孔板测定,需使用96孔UV板,推荐货号YA0602 |
规格 | 100T/96S |
单位 | 盒 |
测定意义:HK(EC 2.7.1.1)广泛存在于动物、植物、微生物和培养细胞中,是葡萄糖分解过程中的第一个关键酶,催化葡萄糖转化为6-磷酸葡萄糖,6-磷酸葡萄糖是糖酵解和磷酸戊糖途径的交叉点。
测定原理:HK 催化葡萄糖合成6-磷酸葡萄糖,6-磷酸葡萄糖脱氢酶进一步催化6-磷酸葡萄糖脱氢生成NADPH,NADPH 在340nm 有特征吸收峰。
需自备的仪器和用品:紫外分光光度计/酶标仪、恒温水浴锅、台式离心机、可调式移液器、微量石英比色皿/96 孔板、研钵、冰和蒸馏水。
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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Zeng Lingjie, Huang Jinxiang, Feng Pixue, Zhao Xuemei, Si Zaiyong, Long Xiufeng, Cheng Qianwei, Yi Yi
IF:
4.2530
Publish_to:
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
PMID:
34989900
Downregulating HK2 inhibits proliferation of endometrial stromal cells through a noncanonical pathway involving phosphorylation of signal transducer and activator of transcription 1 in endometriosis
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Author:
Hou Shuhui, Lei Shating, Peng Haiyan, Weng Lichun, Lv Siji, Li Mingqing, Zhao Dong
IF:
4.1610
Publish_to:
BIOLOGY OF REPRODUCTION
PMID:
35470850
DHA promotes healthy adipose tissue remodeling and carbohydrate utilization in grass carp (Ctenopharyngodon idellus) and largemouth bass (Micropterus salmoides)
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Author:
Handong Li, Lei Song, Mingkui Wei, Xiangtong Yuan, Wenyi Wu, Gen Kaneko, Hong Ji, Jian Sun
IF:
3.7000
Publish_to:
Aquaculture Reports
PMID:
Estrogen Regulates Glucose Metabolism in Cattle Neutrophils Through Autophagy
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Author:
Wang Xinbo, Zhang Yuming, Li Yansong, Tang Mingyu, Deng Qinghua, Mao Jingdong, Du Liyin
IF:
3.4120
Publish_to:
Frontiers in Veterinary Science
PMID:
34912878
Effects of glucose availability on αS1-casein synthesis in bovine mammary epithelial cells
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Author:
Sun Mei, Li Zinan, Xing Yuanyuan, Mu Xiaojia, Cao Yue, Hao Yihong, Yang Jing, Li Dabiao
IF:
3.3380
Publish_to:
JOURNAL OF ANIMAL SCIENCE
PMID:
36222748
Inhibition of PIKfyve Ameliorates the Proliferation and Migration of Vascular Smooth Muscle Cells and Vascular Intima Hyperplasia By Reducing mTORC1 Activity
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Author:
Gu Min, Wang Zhen, Feng Feifei, Yang Yongjian, Sun Xiongshan, Yang Dachun
IF:
3.2710
Publish_to:
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
PMID:
35275098
Skeletal-Muscle-Specific Overexpression of Chrono Leads to Disruption of Glucose Metabolism and Exercise Capacity
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Author:
Shiyi He, Lu Yan, Rongxin Zhu, Hao Wei, Jianxiong Wang, Lan Zheng, Ying Zhang
IF:
3.2510
Publish_to:
Life-Basel
PMID:
36013411
Proteomic and ubiquitinome analysis reveal that microgravity affects glucose metabolism of mouse hearts by remodeling non-degradative ubiquitination
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Author:
Xin Zhang, Xuemei Zhou, Zhiwei Tu, Lihua Qiang, Zhe Lu, Yuping Xie, Cui Hua Liu, Lingqiang Zhang, Yesheng Fu
IF:
2.9000
Publish_to:
PLoS One
PMID:
39541295
Hexokinase Is Required for Sex Pheromone Biosynthesis in Helicoverpa armigera
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Author:
Yanpeng Chang, Yunhui Zhang, Zichen Geng, Shuangyan Yao, Wenli Zhao, Xinming Yin, Shiheng An
IF:
2.7690
Publish_to:
Insects
PMID:
34680657
3‑Bromopyruvic acid regulates glucose metabolism by targeting the c‑Myc/TXNIP axis and induces mitochondria‑mediated apoptosis in TNBC cells
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Author:
Jiachen Li, Jianmin Pan, Yang Liu, Xiaohui Luo, Cheng Yang, Wangfa Xiao, Qishang Li, Lihui Yang, Xiaodong Zhang
IF:
2.7510
Publish_to:
Experimental and Therapeutic Medicine
PMID:
35837063
S100A8/9 modulates perturbation and glycolysis of macrophages in allergic asthma mice
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Author:
Xiaoyi Ji, Chunhua Nie, Yuan Yao, Yu Ma, Huafei Huang, Chuangli Hao
IF:
2.7000
Publish_to:
PeerJ
PMID:
38646478
Network Pharmacology and Molecular Docking Explore the Mechanism of Mubiezi-Yinyanghuo Herb Pair in the Treatment of Rheumatoid Arthritis
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Author:
Fuxue Meng, Xiaomai Tao, Longkuan Li, Wei Jia, Xin Yang, Yuchen Yang
IF:
2.6500
Publish_to:
Evidence-based Complementary and Alternative Medicine
PMID:
38106514
Starch and sucrose metabolism plays an important role in the stem development in Medicago sativa
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Author:
Jierui Wu, Xiaoyu Wang, Lin Bian, Zhenyi Li, Xiaohong Jiang, Fengling Shi, Fang Tang, Zhiqiang Zhang
IF:
2.6000
Publish_to:
FUNCTIONAL PLANT BIOLOGY
PMID:
38739736
High-dose Vitamin C inhibits PD-L1 expression by activating AMPK in colorectal cancer
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Author:
Jia Huang, Su Min, Ruiyang Hong, Mou Zou, Dongyu Zhou
IF:
2.5000
Publish_to:
IMMUNOBIOLOGY
PMID:
40139125
Diclofenac impairs the proliferation and glucose metabolism of triple‑negative breast cancer cells by targeting the c‑Myc pathway
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Author:
Lihui Yang, Jiachen Li, Yongzhuo Li, Yongli Zhou, Ziqian Wang, Dahao Zhang, Jinlu Liu, Xiaodong Zhang
IF:
2.4470
Publish_to:
Experimental and Therapeutic Medicine
PMID:
33850556
Morphology and metabolism of storage substances contribution to alkali stress responses in two contrasting barley cultivars during germination stage
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Author:
Li Jianbo, Jin Yanan, Liu Zhiping, Ba Tu, Wang Wendi, Xu Shoujun
IF:
1.0000
Publish_to:
Journal of Seed Science
PMID:
Respiratory Effects under Air Humidity Stress in Fruit Bodies of Auricularia heimuer
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Author:
Borun Zhu, Yu Bowei, Li Danlin, Fu Chenxi, Ma Shiyu, Ma Huailiang, Wang Yanfeng
IF:
0.5000
Publish_to:
BIOLOGY BULLETIN
PMID:
技术问答
(4)

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您好,可以使用2025-07-08 09:02:59
请问计算公式里的V样总是固定的1ml吗?还是按照自己实际加的量来计算?2024-07-02 01:01:31
您好,计算公式中的V样总根据您实际加的量计算。
这个试剂盒测试的己糖激酶活性是不是单指HK2的活性?还是hk四种类型的总活性?2023-04-14 14:22:29
你好,这个试剂盒可以测人外周血中的己糖激酶吗?2022-09-12 19:32:27
可以,生化试剂盒没有种属限制