中文
英语
丙酮酸激酶(PK)活性检测试剂盒
检测方法:紫外分光光度法 Spectrophotometer
英文名称:Pyruvate Kinase(PK) Activity Assay Kit
储存条件:Store at -20℃，6 months
产品简介
| 中文名称 | 丙酮酸激酶(PK)活性检测试剂盒 |
| 英文名称 | Pyruvate Kinase(PK) Activity Assay Kit |
| 检测方法 | 紫外分光光度法 Spectrophotometer |
| 储存条件 | Store at -20℃，6 months |
| 运输条件 | 冷冻运输 |
| 规格 | 50T/48S |
| 单位 | 盒 |
测定意义：PK（EC 2.7.1.40）广泛存在于动物、植物、微生物和培养细胞中，催化糖酵解过程中的最后一步反应，是糖酵解过程中的主要限速酶之一，也是产生ATP的关键酶之一，因此测定PK活性具有重要意义。
测定原理：PK催化磷酸烯醇式丙酮酸和ADP生成ATP和丙酮酸，乳酸脱氢酶进一步催化NADH和丙酮酸生成乳酸和NAD+，在340nm下测定NADH下降速率，即可反映PK活性。
需自备的仪器和用品：紫外分光光度计、台式离心机、水浴锅、可调式移液器、1mL石英比色皿、研钵、冰和蒸馏水。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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Selection of a galactose-positive mutant strain of Streptococcus thermophilus and its optimized production as a high-vitality starter culture
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Author：
Haimin Hu, gesudu Qimu, Jiaying Nie, Na Wu, Tong Dan
IF：
3.7000
Publish_to：
JOURNAL OF DAIRY SCIENCE
PMID：
38754828
Low-protein diets supplemented with glycine improves pig growth performance and meat quality: An untargeted metabolomic analysis
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Author：
Shengwang Jiang, Wei Quan, Jie Luo, Aihua Lou, Xihong Zhou, Fengna Li, Qingwu W. Shen
IF：
3.4710
Publish_to：
Frontiers in Veterinary Science
PMID：
37143503
Effects of glucose availability on αS1-casein synthesis in bovine mammary epithelial cells
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Author：
Sun Mei, Li Zinan, Xing Yuanyuan, Mu Xiaojia, Cao Yue, Hao Yihong, Yang Jing, Li Dabiao
IF：
3.3380
Publish_to：
JOURNAL OF ANIMAL SCIENCE
PMID：
36222748
Temperature adaptations of the thermophilic snail Echinolittorina malaccana: insights from metabolomic analysis
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Author：
Chen, Ya-qi, Wang, Jie, Liao, Ming-ling, Li, Xiao-xu, Dong, Yun-wei
IF：
3.3120
Publish_to：
JOURNAL OF EXPERIMENTAL BIOLOGY
PMID：
33536302
Knocking Down Ferredoxin 1 Inhibits the Progression of Colorectal Cancer and Regulates Cuproptosis via Mediating the Hippo Signaling Pathway
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Author：
Ying Hu, Haihua Liu, Xiaobin Tan, Xiongjian Wu
IF：
3.0000
Publish_to：
MOLECULAR CARCINOGENESIS
PMID：
39987563
NAMPT promotes hepatitis B virus replication and liver cancer cell proliferation through the regulation of aerobic glycolysis
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Author：
Hui‑Jie Guo, Hong‑Yu Li, Zi‑Hao Chen, Wen‑Jing Zhou, Jia‑Jie Li, Jia‑Yi Zhang, Jing Wang, Xing‑Yan Luo, Ting Zeng, Zhao Shi, Chun‑Fen Mo
IF：
2.9670
Publish_to：
Oncology Letters
PMID：
33777213
Cynaroside prevents macrophage polarization into pro-inflammatory phenotype and alleviates cecal ligation and puncture-induced liver injury by targeting PKM2/HIF-1α axis
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Author：
Liuhua Pei, Yifei Le, Hang Chen, Jiafan Feng, Zhijun Liu, Ji Zhu, Cui Wang, Lin Chen, Xiaobing Dou, Dezhao Lu
IF：
2.8820
Publish_to：
FITOTERAPIA
PMID：
33984439
PKM2 knockout facilitates the activation of the AMPK/KLF4/ACADVL pathway, leading to increased oxidative degradation of fatty acids in TNBC
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Author：
Zhang Linghan, Cheng Li, Ma Yingchao, Li Junlin, Zhong Yue, Zhu Xiuzhi, Leng XiaoMin, Xie Fuhua
IF：
2.8000
Publish_to：
MEDICAL ONCOLOGY
PMID：
40072654
Remodeling of Hepatic Glucose Metabolism in Response to Early Weaning in Piglets
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Author：
Chengbing Yu, Di Wang, Cheng Shen, Zhen Luo, Hongcai Zhang, Jing Zhang, Weina Xu, Jianxiong Xu
IF：
2.7000
Publish_to：
Animals
PMID：
38254359
Cordycepin Inhibits the Growth of Hepatocellular Carcinoma by Regulating the Pathway of Aerobic Glycolysis
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Author：
Ya Chen, Yuan Jiang, Xinyu Huang, Lin Chen, Hao Yang, Jian Zhao, Beibei Liang
IF：
2.6500
Publish_to：
Evidence-based Complementary and Alternative Medicine
PMID：
36467556
A novel bacteriocin RSQ01 with antibacterial activity and its application and metabolomic mechanism in milk preservation
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Author：
Zhang Chengpeng, Huang Zonghai
IF：
2.6000
Publish_to：
MOLECULAR BIOTECHNOLOGY
PMID：
37294531
High-dose Vitamin C inhibits PD-L1 expression by activating AMPK in colorectal cancer
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Author：
Jia Huang, Su Min, Ruiyang Hong, Mou Zou, Dongyu Zhou
IF：
2.5000
Publish_to：
IMMUNOBIOLOGY
PMID：
40139125
Antemortem Stress Regulates Postmortem Glycolysis in Muscle by Deacetylation of Pyruvate Kinase M1 at K141
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Author：
Jiang Shengwang, Shen Qingwu W.
IF：
1.9000
Publish_to：
PROTEIN JOURNAL
PMID：
38605203
KAT8 enhances the resistance of lung cancer cells to cisplatin by acetylation of PKM2
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Author：
Li Zhenyu, Lu Xiangji, Zhang Jing, Liu Tao, Xu Mingzhi, Liu Shuai, Liang Junguo
IF：
1.8000
Publish_to：
ANTI-CANCER DRUGS
PMID：
38771737
Respiratory Effects under Air Humidity Stress in Fruit Bodies of Auricularia heimuer
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Author：
Borun Zhu, Yu Bowei, Li Danlin, Fu Chenxi, Ma Shiyu, Ma Huailiang, Wang Yanfeng
IF：
0.5000
Publish_to：
BIOLOGY BULLETIN
PMID：
技术问答
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注意：
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如果用纯蛋白与化合物孵育，后续测定还可以用丙酮酸激酶试剂盒吗？没有细胞后续还会产生NADH吗?2023-06-19 21:29:20
您好，NADH是反应测定试剂盒中提供的不是细胞后者酶催化产生的。