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碘化丙啶

CAS:No.25535-16-4

英文名称:Propidium Iodide

分子式:C27H34I2N4

分子量:668.39

储存条件:Powder:2-8℃,2 years;Insolvent(母液):-20℃,6 months;-80℃,1 year

纯度:≥98%

碘化丙啶
满3999赠露营车
货号:
IP5030
品牌:
Solarbio
SKU 北京总部 北京海淀 武汉 广州
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使用本产品的案例(仅供参考


In Vitro

Cell (KB cells;5μg/mL PI;15min

PI and FDA co-staining experiments evaluated the effect of PTT in vitro.   KB cells were cultured with Au NCs in 6-well plate at concentration of 100μg/mL based on the content of Au, and then irradiated by 660 nm laser with laser power density of 2 W/cm2 for different times of 0, 5, 10 and 15 min. FDA of 5μg/mL was used to stain the living cells, and the staining time was 15 s.   Meanwhile, PI of 5 μg/mL was used to stain the apoptosis cells, and the staining time was 15 min.

文献来源:Chen Q, Guan G, Deng F, Yang D, Wu P, Kang S, Sun R, Wang X, Zhou D, Dai W, Wang X, Zhang H, He B, Chen D, Zhang Q. Anisotropic active ligandations in siRNA-Loaded hybrid nanodiscs lead to distinct carcinostatic outcomes by regulating nano-bio interactions. Biomaterials. 2020 Apr 3;251:120008. doi: 10.1016/j.biomaterials.2020.120008. Epub ahead of print. PMID: 32388031.


P. ternata seedling(Pinellia ternata root tip cell;  5 μg/mL PI;  10min)

We accurately weigh 100 mg of fluorescein diacetate (FDA) (Solarbio, Beijing, China) and dissolved it in 4 mL of acetone to prepare a 25 mg·mL-1 mother liquor, which was stored away from light at -20 °C.  When in use, FDA storage solution was added to 0.65 mol·L-1 mannitol to obtain FDA dyeing solution in proportion.  10 mg of propidium iodide (PI) (Solarbio, China) was dissolved in 2 mL of 0.65 mol·L-1 mannitol to prepare a 5 mol·L-1 mother liquor.  The FDA (15 μg·mL-1) and PI (5 μg·mL-1) mixtures were used to treat P. ternata seedlings.  P. ternata seedlings were hydroponically cultured and treated with M-Pa-B for 1, 2, 3, and 4 h. After that, the root tips of seedlings were washed three times with deionized water and then dyed with the FDA-PI in the dark for 10 min. The excess dye was washed away thoroughly with deionized water after dyeing.  Ten root samples were collected from five individual plants at each time point.  A laser confocal scanning microscope was used for observation and photography.  The excitation and emission wavelengths are 488 nm and 630 nm, respectively.

来源文献:He Z, Wang Y, Yan Y, Qin S, He H, Mao R, Liang Z. Dynamic analysis of physiological indices and transcriptome profiling revealing the mechanisms of the allelopathic effects of phenolic acids on Pinellia ternata.  Front Plant Sci. 2022 Oct 18;13:1039507.  doi: 10.3389/fpls.2022.1039507.  PMID: 36340387;  PMCID: PMC9635339.



备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.

实验图
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He Z, et al. Front Plant Sci. 2022 Oct 18;13:1039507.
Zhao P,et al. Carbohydr Polym. 2020 Aug 1;241116344.
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