国内地区 :
欢迎光临,Solarbio!登录 | 注册
产品中心
Paclitaxel 紫杉醇

CAS:33069-62-4

分子式:C47H51NO14

分子量:853.92

纯度:HPLC≥98%

Paclitaxel 紫杉醇
同系列5赠2
货号:
IP0020
品牌:
Solarbio
SKU 北京总部 北京海淀 武汉 广州
IP0020-1ml 咨询 咨询 咨询 咨询
IP0020-10mg 咨询 咨询 咨询 咨询
IP0020-50mg 咨询 咨询 咨询 咨询
数量:
-
+
立即购买

紫杉醇可使微管蛋白和组成微管的微管蛋白二聚体失去动态平衡,诱导与促进微管蛋白聚合、微管装配、防止解聚,从而使微管稳定并抑制癌细胞的有丝分裂和防止诱导细胞凋亡,进而有效阻止癌细胞的增殖,起到抗癌作用。


使用本产品的应用案例(仅供参考

In Vitro:

细胞实验:Cytotoxicity determination: The IC50 of cells exposed to PTX was determined using a Cell Counting Kit (CCK)‑8 assay. In brief, cells were inoculated at a concentration of 4x103/100 µl per well and cultured at 37˚C for 12 h in a 96‑well plate. The cells were then maintained at 37˚C with 200 µl fresh medium containing different concentrations of PTX (0‑50 nM) for 48 h. Following this, the cells were incubated with 20 µl CCK‑8 solution at 37˚C for 24 h. Then, the optical density value of each well was detected using a microplate reader at 450 nm.

ROS检测:For ROS detection, 5x105 cells were seeded overnight in a 6‑well plate and then treated with PTX (5 nM) at 37˚C for 48 h. Subsequently, 5 µM carboxy 2'‑7'‑dichlorofluorescein diacetate was added in Hanks' Balanced Salt Solution and incubated with cells at room temperature for 0.5 h. Fresh tissues were harvested, followed by a single cell suspension preparation. Tissue was digested with trypsin to obtain single cell suspension. Cells were then inoculated in a 6‑well plate as aforementioned. The levels of intracellular peroxide were measured using a flow cytometer at 485 nm excitation and 520 nm emission to analyze the ROS generation.

来源文献:Chen M, Su J, Feng C, Liu Y, Zhao L, Tian Y. Chemokine CCL20 promotes the paclitaxel resistance of CD44+CD117+cells via the Notch1 signaling pathway in ovarian cancer. Mol Med Rep. 2021 Sep;24(3):635. doi: 10.3892/mmr.2021.12274. Epub 2021 Jul 19. PMID: 34278466; PMCID: PMC8280726.    


In Vivo:

小鼠,腹腔注射:Xenograft and grouping:In total, 16 male nude mice (age, 6‑8 weeks; weight, 25‑30 g) . Mice were housed  at 25±1˚C with 12‑h light/dark cycles, 50±5% humidity and  free access to food and water. The mice were randomly divided into four groups: i) CD44+CD117++ PTX (Beijing Solarbio Science & Technology Co., Ltd.) group (n=4); ii) CD44+CD117++ PTX + CCL20 (Beijing Solarbio Science & Technology Co., Ltd.) group (n=4); iii) CD44CD117+ PTX group (n=4); and iv) CD44CD117 + PTX + CCL20 group (n=4). For xenografts, CD44+CD117+(2x106) subsets or CD44CD117subsets cells (2x106) suspended in 0.5 ml PBS were subcutaneously injected into the left flank of each nude mouse to establish tumors. Then, PTX (10 mg/kg) and/or CCL20 (1 mg/kg) were injected intraperitoneally simultaneously on days 1, 3 and 7. Tumor size was monitored on the 3rd, 7th, 14th, 21st and 28th days (15). At the end of the experiment and under continuous anesthesia, the animals were euthanized by an overdose of pentobarbital (125 mg/kg). 

来源文献:Chen M, Su J, Feng C, Liu Y, Zhao L, Tian Y. Chemokine CCL20 promotes the paclitaxel resistance of CD44+CD117+cells via the Notch1 signaling pathway in ovarian cancer. Mol Med Rep. 2021 Sep;24(3):635. doi: 10.3892/mmr.2021.12274. Epub 2021 Jul 19. PMID: 34278466; PMCID: PMC8280726.    


备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
实验图
.
Chen M, et al. Mol Med Rep. 2021 Sep;24(3):635.
Chen M, et al. Mol Med Rep. 2021 Sep;24(3):635.
Chen M, et al. Mol Med Rep. 2021 Sep;24(3):635.
注意:
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响,相关处理只限于产品本身,不涉及其他赔偿。
为科研服务、为生命尽责
联系方式
地址: 北京市通州区马驹桥联东U谷85号
客服电话: 400-968-6088
技术邮箱: service@solarbio.com
采购邮箱: purchase@solarbio.com
销售邮箱: sales-china@solarbio.com
Copyright © 2004-2022 北京索莱宝科技有限公司. 版权所有.
 京ICP备12051307号-7 
京公网安备 11011202000391号