中文
英语
氯霉素
CAS:No.56-75-7
分子式:C11H12Cl2N2O5
分子量:323.13
储存条件:2-8℃，有效期4年
纯度:≥99.00%
英文名称:Chloramphenicol
产品简介
| 别名 | 左旋霉素;左霉素;D(-)-苏-1-对硝基苯基-2-二氯乙酰胺基-1,3-丙二醇;D-苏式-(-)-N-[α-(羟基甲基)-β-羟基-对硝基苯乙基]-2,2-二氯乙酰胺 |
| 英文名称 | Chloramphenicol |
| CAS | No.56-75-7 |
| 分子式 | C11H12Cl2N2O5 |
| 分子量 | 323.13 |
| 储存条件 | 2-8℃，有效期4年 |
| 外观(性状) |
白色至类白色结晶粉末
|
| 纯度 | ≥99.00% |
| 溶解性 | Solubility 50mg/ml in Ethanol |
| 危险代码 | R：23/24/25-42/43-60/61-45 S：36/37/39 |
| 规格 | 5g 10g |
| 单位 | 瓶 |
说明：配制组织培养基。
氯霉素，英文名称Chloramphenicol，一种细菌性广谱抗生素，可在肽键转移酶催化形成肽键的过程中抑制50S核糖体上mRNA的翻译，从而抑制革兰氏阴性和革兰氏阳性细菌的生长。此外氯霉素还具有立克次体属微生物抗性以及鹦鹉热-淋巴肉芽肿类衣原体抗性等，因此氯霉素可用于细菌筛选。
对氯霉素乙酰转移酶基因（即CAT gene）进行乙酰化可抵制氯霉素的抗性。基于该原理，分子生物学实验中，氯霉素可作为一种筛选药物用于筛选含有氯霉素抗性基因的转化细胞。
推荐有效工作浓度为5-20µg/ml。
溶解性： 溶于甲醇、乙醇、丁醇、丙酮和乙酸乙酯，微溶于醚，不溶于苯和石油醚。
储存液制备：
称取1g氯霉素溶于20ml 100%乙醇充分溶解后得到50mg/ml的母液，0.22µM有机相滤膜过滤除菌后，分装成小量后-20°C冻存，避免反复冻融，一年有效。
【注】：氯霉素储存液不可高压灭菌。
注意事项:
(1)用于细胞实验，溶解后，须用一次性针头滤器过滤除菌。
(2)为了您的安全和健康，请穿实验服并戴一次性手套操作。
备注：产品信息可能会有优化升级。请以实际标签信息为准。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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Tingxiu Yang, Jia Li, Yuanyuan Zhang, Zhaohui Deng, Guzhen Cui, Jun Yuan, Jianchao Sun, Xiaojuan Wu, Dengxiong Hua, Song Xiang, Zhenghong Chen
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3.7000
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PLoS One
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Yi Tao, Lin Chen, Meiling Pan, Fei Zhu, Jizhong Yan
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3.6450
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JOURNAL OF SEPARATION SCIENCE
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34240804
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Jun Chen, Fei Zhu, Meiling Pan, Qing Liu, Lin Chen, Yi Tao
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JOURNAL OF SEPARATION SCIENCE
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35384303
Acceleration of emergence of E. coli antibiotic resistance in a simulated sublethal concentration of copper and tetracycline co-contaminated environment
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AMB Express
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2.8000
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EUROPEAN JOURNAL OF MEDICAL RESEARCH
PMID：
39910594
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YEAST
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37565669
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ARCHIVES OF MICROBIOLOGY
PMID：
34057548
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Zhu Ling, Yu Jiang-Yan, Xu Qing-Fang, Bai Xu, Gao Xiu, Zhang Li-Fang, Liu Wei-Liang, Wang Hao-Han, Cai Jian
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ARCHIVES OF MICROBIOLOGY
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39419831
cydA, spdC, and mroQ are novel genes involved in the plasma coagulation of Staphylococcus aureus
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Dong Luo, Wei Wang, Qiang Chen, Linfeng Peng, Xiaomei Hu, Kaisen Chen
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MICROBIOLOGY AND IMMUNOLOGY
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34050992
Influence of Flagella on Salmonella Enteritidis Sedimentation, Biofilm Formation, Disinfectant Resistance, and Interspecies Interactions
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Huixue Hu, Jingguo Xu, Jingyu Chen, Chao Tang, Tianhao Zhou, Jun Wang, Zhuangli Kang
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1.9000
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Foodborne Pathogens and Disease
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39513945
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Yankang Wang, Hongmei Zhang, Wenjing Shi, Yongheng Rong, Weian Mao, Linhan Wang, Wenzhu Tang, Yun Kong, Shengjun Wang
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1.4000
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PROTEIN EXPRESSION AND PURIFICATION
PMID：
40120704
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