二胺氧化酶活性检测试剂盒
检测方法:微量法 Spectrophotometer/Microplate Reader
英文名称:Diamine Oxidase(DAO) Activity Assay Kit
储存条件:Store at 2-8℃,6 months


产品简介
中文名称 | 二胺氧化酶活性检测试剂盒 |
英文名称 | Diamine Oxidase(DAO) Activity Assay Kit |
别名 | 二胺氧化酶试剂盒 二胺氧化酶测试盒 |
检测方法 | 微量法 Spectrophotometer/Microplate Reader |
储存条件 | Store at 2-8℃,6 months |
规格 | 100T/48S |
单位 | 盒 |
测定意义:DAO(EC1.4.3.6)广泛存在于动物(肠粘膜、肺、肝脏、肾脏等)、植物和微生物中。催化多胺氧化为醛,其活性与核酸和蛋白合成密切相关,能够反映肠道机械屏障的完整性和受损伤程度。
测定原理:DAO催化尸胺产生醛和过氧化氢,外源添加过量的辣根过氧化物酶,催化过氧化氢氧化邻联茴香胺生成有色物质,在460nm处有特征吸收峰,通过测定该波长吸光度增加速率,计算DAO活性。
需要的仪器,耗材:天平、低温离心机、可见分光光度计/酶标仪、微量石英比色皿/96孔板、蒸馏水。
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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