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丙酮酸脱氢酶(PDH)活性检测试剂盒
检测方法:微量法 Spectrophotometer/Microplate Reader
英文名称:Pyruvate Dehydrogenase(PDH) Activity Assay Kit
储存条件:Store at -20℃，6 months
产品简介
| 中文名称 | 丙酮酸脱氢酶(PDH)活性检测试剂盒 |
| 英文名称 | Pyruvate Dehydrogenase(PDH) Activity Assay Kit |
| 检测方法 | 微量法 Spectrophotometer/Microplate Reader |
| 储存条件 | Store at -20℃，6 months |
| 规格 | 100T/96S |
| 单位 | 盒 |
测定意义：PDH（EC 1.2.4.1）广泛存在于动物、植物、微生物和培养细胞中，是丙酮酸脱氢酶复合体(PDHC)催化丙酮酸氧化脱羧的限速酶，催化丙酮酸脱梭生成羟乙基-TPP，把糖酵解和三羧酸循环连接起来。
测定原理：PDH催化丙酮酸脱氢，同时还原2,6-二氯酚靛酚（2,6-DCPIP），从而导致600nm光吸收的减少。
需要的仪器，耗材:可见分光光度计/酶标仪、水浴锅、台式离心机、可调式移液器、微量石英比色皿/96孔板、研钵、冰和蒸馏水。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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