硝酸纤维素膜NC膜(0.45um)
英文名称:Immobilon-NC Membrane,Triton-free,mixed cellulose esters,0,45 μm
储存条件:常温


产品简介
英文名称 | Immobilon-NC Membrane,Triton-free,mixed cellulose esters,0,45 μm |
储存条件 | 常温 |
规格 | 15*20cm 30cm*300cm |
单位 | 张/卷 |
用于科研实验研究耗材。
硝酸纤维素膜NC膜优点
硝酸纤维素膜是世界上实用最为广泛的特异性转移介质之一,硝酸纤维素膜采用100%纯硝酸纤维素材料制成,确保了最高的结合能力。
使用方便,操作简单,兼容多种检测方法,与PVDF膜相比,不需要甲醇浸泡过程,这样使得那些需要亲水环境下的蛋白质转移。在转移前,仅需要在水中简单润湿,然后置于转移缓冲液中,不需要其他的预湿步骤了。
背景低
除了具有高结合力外,硝酸纤维素膜本身产生的背景非常低,膜特有的表面性质保证了优异的信噪比,在膜洗脱时不需要严格的洗脱条件。
小分子蛋白的高截留率
硝酸纤维膜有多种孔径以供选择,按照不同的使用要求进行选择相应的孔径的膜,0.22um的硝酸纤维素膜通过小孔径来截留分子量小于20kD的蛋白质分子。0.45um的膜是较大分子蛋白和核酸的理想选择,0.1um 的膜常用于分子量小于7kD的蛋白质。
经实验证明,该膜主要优势在于结合在膜上的分子识别活性长达5年。
注意事项:
1、硝酸纤维素膜是亲水膜,不需要在乙醇、甲醇等溶液中浸泡活化。
2、硝酸纤维素膜比较脆,容易破碎,操作要小心。
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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Octopamine signaling is involved in the female postmating state in Nilaparvata lugens Stål (Hemiptera: Delphacidae)
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IF:
1.6980
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ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
PMID:
34164848
Enhancing hemophilia A gene therapy by strategic F8 deletions in AAV vectors
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IF:
1.5000
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Blood Science
PMID:
39949501
Immunogenicity and protection against Glaesserella parasuis serotype 13 infection after vaccination with recombinant protein LolA in mice
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IF:
1.1050
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JOURNAL OF VETERINARY MEDICAL SCIENCE
PMID:
36216558
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请问这个可以做侧向层析的反应垫吗?谢谢?2022-09-30 17:05:10
您好,这个NC膜不建议用于侧向层析检测反应,第一是孔径较小,容易造成堵膜;第二是没有背衬,做侧向层析时需要粘贴在PVC底板上,没有背衬无法阻隔胶内物质迁移进入膜内;第三是尺寸大小不合适。