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英语
产品简介
| 英文名称 | Rat tail tendon collagen type |
| 中文名称 | 鼠尾胶原蛋白Ⅰ型 |
| 外观(性状) | 液体 |
| 溶解性 | 5mg/ml in 0.006mol/L Acetic acid |
| 储存条件 | 2-8℃，1 year |
| 浓度 | 5mg/ml |
| 规格 | 10mg |
| 单位 | 支 |
索莱宝鼠尾胶原蛋白 I 型(rattailtendon collagen type I)是通过 Birkedal-Hansen 的方法，通过醋酸抽提、氯化钠沉淀、磷酸氢二钠沉淀等步骤制备的。本公司鼠尾胶原蛋白可用于包被细胞培养器皿，培养一些在普通细胞培养器皿中不易贴壁的细胞。也可用于制备三维胶，模拟真实的生长环境，使细胞在三维环境中生长。
1，在使用索莱宝鼠胶原蛋白 I 型包被的细胞培养皿中检查到 PC-12 细胞的贴壁和生长。
2，在 1mg/ml 浓度以上，pH 为 7 左右时可形成具有一定强度的三维胶，检查到 NIH-3T3 细胞在三维胶内正
常生长、PC-12 细胞在三维胶表面正常生长。
使用方法：
1、细胞培养器皿的表面包被 推荐浓度： 1-5ug/cm2
以包被浓度为 2 ug/cm2为例：用无菌 0.006mol/L(0.36g/L) 乙酸将胶原蛋白稀释到 0.012mg/ml。
<img alt=" src="/images/upload/image/20160507/20160507104544_30600.png"/>
确保胶原蛋白溶液铺满器皿的表面，开盖在超净台上过夜晾干。 也可以在室温放置 1小时后，用PBS洗3-4次后直接使用。包被好的器皿在 4-25℃至少可保存3个月以上的时间。
2、三维胶原的制备
鼠尾胶原蛋白I型在浓度1mg/mL以上，pH 7左右时可形成具有一定强度三维胶，建议成胶浓度1-2mg/mL。胶原蛋白溶解于0.006mol/L 乙酸中，在成胶过程中需要加入0.06×体积的 0.1mol/L NaOH 来中和。
需要的溶液（均需要无菌、预冷）：10×PBS（可含10 mg/L的酚红用于pH指示）或10×培养液，0.1mol/L NaOH，0.1mol/L 乙酸(一般不用)，双蒸水
A． 不含细胞的三维胶原的制备（以配制1mL，1mg/mL三维胶为例）：将200μL鼠尾胶原蛋白I型(5mg/mL)加到置于冰浴的离心管中，加入 690μL H2O。然后加到12μL 0.1mol/L NaOH中（如果反过来把12μL 0.1mol/L NaOH加到胶原溶液中，会由于NaOH不能迅速混匀而产生局部的胶原凝结），立即混匀。再加入100μL 10×PBS或10×培养液，混匀后立即加到培养器皿中(混匀后pH为7左右，如果PBS或培养液中没有加酚红，初次使用时需要用pH试纸测试)。将培养器皿在室温(25度左右)下放置20分钟待胶凝固后，转移到培养箱内。如果配制中使用的是10×PBS，使用前需要加入适当体积的细胞培养液预平衡。
B．含细胞的三维胶原的制备（以配制1 mL，1mg/mL三维胶为例）：准备好悬浮于培养液的细胞，并放置于冰浴中。将200μL鼠尾胶原蛋白I型 (5mg/mL)加到12μL 0.1mol/L NaOH中（如果反过来把12μL 0.1mol/L NaOH加到胶原溶液中，会由于NaOH不能迅速混匀而产生局部的胶原凝结），立即混匀。再加入23μL 10×PBS或10×培养液，混匀(混匀后pH为7左右，如果PBS或培养液中没有加酚红，初次使用时需要用pH试纸测试)。加入760μL的细胞悬浮液，混匀后立即加到培养器皿中。将培养器皿在室温下放置20分钟待胶凝固后，加入适当体积的细胞培养液，转移到培养箱中培养。
注意事项：
鼠尾胶原蛋白I型在室温下pH中性时可迅速成胶，在操作过程中要尽量保持低温。
备注：产品信息可能会有优化升级。请以实际标签信息为准。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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Linshan Xu, Yuyang Wang, Jianping Wang, Jianglong Zhai, Li Ren, Guoying Zhu
IF：
5.9240
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
PMID：
34502232
Osteocyte Apoptosis Contributes to Cold Exposure-induced Bone Loss.
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Author：
Jingke Du, Zihao He, Junqi Cui, Hanjun Li, Mingming Xu, Shuhong Zhang, Shuangyan Zhang, Mengning Yan, Xinhua Qu, Zhifeng Yu
IF：
5.8900
Publish_to：
Frontiers in Bioengineering and Biotechnology
PMID：
34858954
A sustained release of BMP2 in urine-derived stem cells enhances the osteogenic differentiation and the potential of bone regeneration
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Wu Shuang, Chen Zhao, Yu Xi, Duan Xin, Chen Jialei, Liu Guoming, Gong Min, Xing Fei, Sun Jiachen, Huang Shishu, Xiang Zhou
IF：
5.7630
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Regenerative Biomaterials
PMID：
35529046
Recombinant humanized IgG1 maintain liver triglyceride homeostasis through Arylacetamide deacetylase in ApoE−/− mice
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Author：
Sujun Xiao, Run Lin, Rui Duan, Zhonghao Li, Dongmei Tang, Xianyan Liu, Yan Liu, Ming Zhao
IF：
5.7140
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INTERNATIONAL IMMUNOPHARMACOLOGY
PMID：
35397394
A variable mineralization time and solution concentration intervene in the microstructure of biomimetic mineralized collagen and potential osteogenic microenvironment
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Author：
Xiujie Zhu, Haotian Bai, He Liu, Zhonghan Wang, Yao Wang, Jiaxin Zhang, Jiaqi Liu, Hui Wang, Jincheng Wang
IF：
5.7000
Publish_to：
Frontiers in Bioengineering and Biotechnology
PMID：
38125304
C-C Motif Chemokine Ligand 5 (CCL5) Promotes Irradiation-Evoked Osteoclastogenesis
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Author：
Jing Wang, Fanyu Zhao, Linshan Xu, Jianping Wang, Jianglong Zhai, Li Ren, Guoying Zhu
IF：
5.6000
Publish_to：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
PMID：
38003358
Bioaccessibility and Intestinal Transport of Tebuconazole in Table Grape by Using In Vitro Digestion Models
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Author：
Xiaowei Liu, Ying Han, Ouli Xiao, Weiye Cui, Jieyin Chen, Xiaofeng Dai, Minmin Li, Zhiqiang Kong
IF：
5.5610
Publish_to：
Foods
PMID：
36496737
2-Methoxybenzoic acid ameliorates arterial thrombosis via inhibiting carbon anhydrase activity in platelet
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Author：
Yunchong Liu, Zhengde Zhao, Xiuyi Huang, Ying Xiao, Na Li, Wenchao Yang, Ruijia Feng, Weiqi Feng, Ting Long, Haoliang Wu, Guiyan Peng, Sifan Chen, Guangqi Chang, Kan Huang, Zilun Li
IF：
5.5000
Publish_to：
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
PMID：
40056982
Dihydroartemisinin inhibits the activation and proliferation of hepatic stellate cells by regulating miR 29b 3p
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Author：
Sheng Huan, Sumin Sun, Shilian Song, Jin Dai, Guining Zhu, Yanling Zhong, Yihao Ji, Shizhong Zheng, Guoping Yin
IF：
5.3140
Publish_to：
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
PMID：
37026504
S-Nitrosylation-mediated coupling of DJ-1 with PTEN induces PI3K/AKT/mTOR pathway-dependent keloid formation
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Author：
Lv Dongming, Xu Zhongye, Cheng Pu, Hu Zhicheng, Dong Yunxian, Rong Yanchao, Xu Hailin, Wang Zhiyong, Cao Xiaoling, Deng Wuguo, Tang Bing
IF：
5.3000
Publish_to：
Burns & Trauma
PMID：
38116467
Identifying cancer cell-secreted proteins that activate cancer-associated fibroblasts as prognostic factors for patients with pancreatic cancer
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Author：
Qiankun Luo, Jiayin Liu, Qiang Fu, Xu Zhang, Pengfei Yu, Pan Liu, Jiali Zhang, Huiyuan Tian, Song Chen, Hongwei Zhang, Tao Qin
IF：
5.2950
Publish_to：
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
PMID：
36282889
Absorption, Metabolism, and Excretion of Cajaninstilbene Acid
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Author：
Lisha Wang, Zhi Wang, Tianji Xia, Fangrui Cao, Linhu Ye, Ruile Pan, Suwei Jin, Mingzhu Yan, Qi Chang
IF：
5.2790
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
PMID：
33560125
The lactate receptor GPR81 mediates hepatic lipid metabolism and the therapeutic effect of metformin on experimental NAFLDs
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Author：
Guanglu Wu, Yufeng Dai, Yongheng Yan, Xu Zheng, Hao Zhang, Haitao Li, Wei Chen
IF：
5.1950
Publish_to：
EUROPEAN JOURNAL OF PHARMACOLOGY
PMID：
35430208
WDR73 Depletion Destabilizes PIP4K2C Activity and Impairs Focal Adhesion Formation in Galloway–Mowat Syndrome
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Author：
Hongyan Li, Fang Liu, Hanzhe Kuang, Hua Teng, Siyi Chen, Sijing Zeng, Qimin Zhou, Zhaokai Li, Desheng Liang, Zhuo Li, Lingqian Wu
IF：
5.1680
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Biology-Basel
PMID：
36290302
Metabolic activation and cytotoxicity of metaxalone mediated by cytochrome P450 enzymes and sulfotransferases
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Author：
He Liu, Zixia Hu, Ningning Han, Yi Yang, Guode Zhao, Mengdie Su, Yue Zhang, Weiwei Li, Ying Peng, Jiang Zheng
IF：
5.1000
Publish_to：
CHEMICO-BIOLOGICAL INTERACTIONS
PMID：
37442290
Preparation and Characterization of Nanocomposite Hydrogels Based on Self-Assembling Collagen and Cellulose Nanocrystals
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Author：
Ya Li, Xiaotong Dong, Lihui Yao, Yajuan Wang, Linghui Wang, Zhiqiang Jiang, Dan Qiu
IF：
5.0000
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Polymers
PMID：
36904549
Establishment and characterization of a highly metastatic hepatocellular carcinoma cell line
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Author：
Jiacheng Huang, Mengqing Sun, Menglan Wang, Anning Yu, Huilin Zheng, Chiwen Bu, Jie Zhou, Yu Zhang, Yiting Qiao, Zhenhua Hu
IF：
4.9000
Publish_to：
Bioengineered
PMID：
38184822
DNA hypermethylation-induced suppression of ALKBH5 is required for folic acid to alleviate hepatic lipid deposition by enhancing autophagy in an ATG12-dependent manner
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Author：
Chaoqun Huang, Yaojun Luo, Youhua Liu, Jiaqi Liu, Yushi Chen, Botao Zeng, Xing Liao, Yuxi Liu, Xinxia Wang
IF：
4.8000
Publish_to：
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
PMID：
39993647
DDR1/2 enhance KIT activation and imatinib resistance of primary and secondary KIT mutants in gastrointestinal stromal tumors
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Author：
Anbu Liu, Shaoting Zhang, Ming Wang, Liangying Zhang, Shidong Xu, Ahmad Nasimian, Shujing Li, Sien Zhao, Xu Cao, Jinhai Tian, Yuanyuan Yu, Zhaoyang Fan, Kun Xiao, Hui Zhao, Julhash U. Kazi, Lijun Ma, Jianmin Sun
IF：
4.6000
Publish_to：
MOLECULAR CARCINOGENESIS
PMID：
37737519
Synergistic Bipolar Irreversible Electroporation for Tumor Ablation without Muscle Contraction
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Author：
Yancheng Wang, Kun Qian, Xinlei Liu, Qiang Yang, Yizhen Lei, Liming Zhu, Chenguo Yao, Quan Zhou, Huawen Liu, Shoulong Dong
IF：
4.4000
Publish_to：
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
PMID：
39028604
Reveals of candidate active ingredients in Justicia and its anti-thrombotic action of mechanism based on network pharmacology approach and experimental validation
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Author：
Hong Zongchao, Zhang Ting, Zhang Ying, Xie Zhoutao, Lu Yi, Yao Yunfeng, Yang Yanfang, Wu Hezhen, Liu Bo
IF：
4.3800
Publish_to：
Scientific Reports
PMID：
34433871
Mechanistic study of cytochrome P450 enzyme-mediated cytotoxicity of psoralen and isopsoralen
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Author：
Shuaishuai Chen, Weiyu Guo, Huan Liu, Jiang Zheng, Dingyan Lu, Jia Sun, Chun Li, Chunhua Liu, Yonglin Wang, Yong Huang, Wen Liu, Yongjun Li, Ting Liu
IF：
4.3000
Publish_to：
FOOD AND CHEMICAL TOXICOLOGY
PMID：
37660943
Evidence for adduction of biologic amines with reactive metabolite of 8-epidiosbulbin E acetate in vitro and in vivo
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Author：
Na Zhang, Shenzhi Zhou, Zhengyu Zhang, Wei Li, Ying Peng, Jiang Zheng
IF：
4.2710
Publish_to：
TOXICOLOGY LETTERS
PMID：
35680040
An integrated microfluidic device for rapid and high-sensitivity analysis of circulating tumor cells
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Author：
Jianing Jiang, Hui Zhao, Weiliang Shu, Jing Tian,
IF：
4.2590
Publish_to：
Scientific Reports 7, Article number: 42612 (2017)
PMID：
28198402
Histamine promotes adhesion of neutrophils by inhibition of autophagy in dairy cows with subacute ruminal acidosis
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Author：
Kexin Wang, Zhenai Sun, Yunfei Li, Mingchao Liu, Juan J. Loor, Qianming Jiang, Guowen Liu, Zhe Wang, Yuxiang Song, Xinwei Li
IF：
4.2250
Publish_to：
JOURNAL OF DAIRY SCIENCE
PMID：
35940921
Microcystin-leucine arginine blocks vasculogenesis and angiogenesis through impairing cytoskeleton and impeding endothelial cell migration by downregulating integrin-mediated Rho/ROCK signaling pathway
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Author：
Wang Qilong, Chen Guoliang, Zhang Qian, Wang Mingxing, Wang Guixue, Hu Tingzhang
IF：
4.2230
Publish_to：
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
PMID：
34244946
Downregulation of CYRI-B promotes migration, invasion and EMT by activating the Rac1-STAT3 pathway in gastric cancer
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Author：
Guangyao Liu, Yujian Xia, Huijin Wang, Xinghan Jin, Songyao Chen, Wei Chen, Changhua Zhang, Yulong He
IF：
4.1450
Publish_to：
EXPERIMENTAL CELL RESEARCH
PMID：
36584745
In Vitro and In Vivo Metabolic Activation of Tolterodine Mediated by CYP3A
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Author：
Aixuan Wang, Qiang Zhao, Minglu Liu, Yang Wang, Guode Zhao, Weiwei Li, Ying Peng, Jiang Zheng
IF：
4.1000
Publish_to：
CHEMICAL RESEARCH IN TOXICOLOGY
PMID：
36795936
Design of a microfluidic lung chip and its application in assessing the toxicity of formaldehyde
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Author：
Man Su, Xiang Li, Zezhi Li, Chenfeng Hua, Pingping Shang, Junwei Zhao, Kejian Liu, Fuwei Xie
IF：
4.0190
Publish_to：
TOXICOLOGY MECHANISMS AND METHODS
PMID：
36573377
Metabolic Activation of Perampanel Mediated by CYP1A2
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Author：
Zifang Ding, Xu Wang, Ning Zhang, Chen Sun, Guode Zhao, Ying Peng, Jiang Zheng
IF：
3.9730
Publish_to：
CHEMICAL RESEARCH IN TOXICOLOGY
PMID：
35200000
Dll4/Notch1 signalling pathway is required in collective invasion of salivary adenoid cystic carcinoma Corrigendum in /10.3892/or.2022.8268
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Author：
Ke Wang, Hua‑Yang Fan, Xing Pang, Mei Zhang, Xiang‑Hua Yu, Jia‑Shun Wu, Bing‑Jun Chen, Jian Jiang, Xin‑Hua Liang, Ya‑Ling Tang
IF：
3.9060
Publish_to：
ONCOLOGY REPORTS
PMID：
33469672
Far-infrared radiation alleviates steatohepatitis and fibrosis in metabolic dysfunction-associated fatty liver disease
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Author：
Xu Tianyi, Fu Haijing, Zhao Wumei, Shan Shijun
IF：
3.8000
Publish_to：
Scientific Reports
PMID：
39164313
Metabolic Activation and Cytotoxicity of Labetalol Hydrochloride Mediated by Sulfotransferases
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Author：
Lan Yang, Lihua Xin, Junzu Shi, Wei Li, Min Tian, Zixia Hu, Ying Peng, Jiang Zheng
IF：
3.7390
Publish_to：
CHEMICAL RESEARCH IN TOXICOLOGY
PMID：
33872499
Hydrogel Composite Incorporating Deferoxamine-Loaded Gelatin-Based Microspheres Enhance Angiogenesis Ability of Dental Pulp Stem Cells
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Author：
Jie Wang, Fan Yang, Ruting Chen, Xinyue Yang, Jingjing Wang, Hongyan Zhang
IF：
3.7000
Publish_to：
ACS Omega
PMID：
40191326
Human dental follicle cell-derived conditioned media enhance periodontal regeneration by regulating the osteogenic differentiation and inflammation of periodontal ligament stem cells and macrophage polarization
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Author：
Gao Yichen, He Xianyi, Deng Yuyao, Wang Xia, Shen Jie, Xu Wei, Cai Rui, Lan Xiaorong, Chen Junliang, He Yun
IF：
3.5000
Publish_to：
MOLECULAR AND CELLULAR BIOCHEMISTRY
PMID：
40175780
Btbd8 deficiency exacerbates bleomycin-induced pulmonary fibrosis in mice by enhancing myofibroblast accumulation and inflammatory responses
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Author：
Xiaoqiong Yang, Qiman Dong, Xingyuan Tong, Xiaoling Du, Lingyi Chen
IF：
3.3000
Publish_to：
EXPERIMENTAL CELL RESEARCH
PMID：
40049313
Contribution of platelets to disruption of the blood-brain barrier during arterial baroreflex dysfunction
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Author：
Bowen Shen, Lili Yang, Xiaoli Jia, Deping Kong, Lei Jing, Yongfeng Gao, Shan Gao, Ruimin Chen, Fengbao Chen, Chunyu Zhao, Yue Li, Rui Tan, Xiaomin Zhao
IF：
3.1000
Publish_to：
MICROVASCULAR RESEARCH
PMID：
38493885
Dihydroartemisinin protects blood–brain barrier permeability during sepsis by inhibiting the transcription factor SNAI1
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Author：
Fuhong Liu, Jing Liu, Hongjie Xiang, Zongguo Sun, Yan Li, Xiao Li, Yanjun Liu, Ju Liu
IF：
2.9630
Publish_to：
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
PMID：
35651290
New considerations in selecting donors for dental pulp stem cells: a pilot study
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Author：
Mingchang Hu, Qianqian Zhang, Jidong Xu, Linlin Xu, Xuecheng Xu, Jiajia Wang, Yu Song
IF：
2.9000
Publish_to：
Biomedical Engineering Online
PMID：
40119437
On-chip construction of a fully structured scaffold-free vascularized renal tubule
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Author：
Zhu Yuntian, Shi Zhengdi, Ding Weiping, Li Chengpan
IF：
2.8000
Publish_to：
BIOMEDICAL MICRODEVICES
PMID：
36826720
Establishment of an in Vitro Three-Dimensional Vascularized Micro-Tumor Model and Screening of Chemotherapeutic Drugs
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Author：
Qian Ye, Juanru Wang, Boyang Wang, Min Zhao, Zhengsheng Wu, Xiaoli Liu
IF：
2.7000
Publish_to：
TECHNOLOGY IN CANCER RESEARCH & TREATMENT
PMID：
39311637
The expression of membrane-bound complement regulatory proteins CD46, CD55 and CD59 in oral lichen planus
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Author：
Lulu Li, Beibei Cong, Xixi Yu, Songsong Deng, Mengjia Liu, Yiheng Wang, Wanchun Wang, Meihua Gao, Yingjie Xu
IF：
2.6350
Publish_to：
ARCHIVES OF ORAL BIOLOGY
PMID：
33529836
The Nf1-Q181X point mutation induces M2 macrophage polarization via the AKT/STAT pathway to promote smooth muscle cell proliferation and migration
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Author：
Yang Yang, Yao Zhichao, Huo Lirong
IF：
2.6000
Publish_to：
MOLECULAR BIOLOGY REPORTS
PMID：
39215899
Cepharanthine Mitigates NIBV-induced Pyroptosis via the MDA5/NF-κB/NLRP3 Signaling Pathway
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Author：
Zhenni Liu, Ruoyun Tang, Mengbing Ding, Meiqin Wu, Ying Li, Cheng Huang, Qiurong Qi, Ping Liu, Xiaona Gao, Xiaoquan Guo
IF：
2.4000
Publish_to：
VETERINARY MICROBIOLOGY
PMID：
40273551
Structural characteristics, antioxidant and hypoglycemic activities of polysaccharides from Mori Fructus based on different extraction methods
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Author：
Xilong Wang, Lixin Yue, Fan Zhang, Xiaojuan Bao, Wanghua Song, Zheng Li
IF：
2.3000
Publish_to：
Journal of Cosmetic Dermatology
PMID：
37310204
3D cell/scaffold model based on aligned-electrospun-nanofiber film/hydrogel multilayers for construction of anisotropic engineered tissue
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Author：
Zhang Jing-Yi, Cheraga Nihad, Huang Ning-Ping
IF：
1.9160
Publish_to：
Biointerphases
PMID：
36216595
技术问答
（4）
注意：
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。
京公网安备 11011202000391号
I型胶原三维培养的细胞还可以去除胶原后收集起来么?2023-11-19 19:00:19
您好，不建议再收集细胞，因为胶原可能无法完全去除。
胶原凝固需要多久？怎么判断凝固情况？我室温放置一个小时，感觉细胞好像固定住了，但周围凝胶还可以流动。?2023-06-19 14:49:02
您好，该产品在配制三维胶时，室温（25℃），PH为7的条件下，20min左右凝固，凝固状态可以将孔板或者其他容器拿起，倒扣在平面上观察，没有流下来的液体即为凝固好。
以包被浓度为 2 ug/cm2为例：用无菌 0.006mol/L(0.36g/L) 乙酸将胶原蛋白稀释到 0.012mg/ml你们提供无菌 0.006mol/L(0.36g/L) 乙酸吗?2023-02-09 09:24:20
您好，没有的，无菌乙酸需要单独购买或配制。
请问C8062-10mg的纯度是多少?2022-10-16 14:38:39
您好，该试剂是液体的，浓度为5mg/ml。