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佛波酯

CAS:No.16561-29-8

英文名称:Phorbol 12-Myristate 13-Acetate

分子式:C36H56O8

分子量:616.83

储存条件:Powder:-20℃,2 years;Insolvent(母液):-20℃,6 months;-80℃,1 year

纯度:HPLC≥98%

佛波酯
小分子同系列4赠1
货号:
IP1010
品牌:
Solarbio
SKU 北京总部 北京海淀 武汉 广州
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使用本产品的应用案例(仅供参考

In Vitro

Cell(THP-1 cells;100 ng/mL PMA;24h

The human monocytic cell line (THP-1) was cultured in RPMI medium 1640 supplemented with 10% FBS and 50 μg/mL penicillin/streptomycin in the incubator at 37°C with 5% CO2 balanced with air.  THP-1 monocytic cells were treated with 100 ng/mL phorbol myristate acetate (PMA) (Solarbio, Beijing, China) for 24 h to induce macrophage phenotype.

文献来源:Lai D, Zhu K, Li S, Xiao Y, Xu Q, Sun Y, Yao P, Ma D, Shu Q. SARS-CoV-2 N Protein Triggers Acute Lung Injury via Modulating Macrophage Activation and Infiltration in in vitro and in vivo.  J Inflamm Res. 2023 Apr 28;16:1867-1877.  doi: 10.2147/JIR.S405722.  PMID: 37143821;  PMCID: PMC10153437.


Cell(the peripheral blood lymphocytes50 ng/ml PMA, 5h

Blood from the M6-immunized (three doses) mice or rhesus monkeys was collected at and 28 days post-infection. Red blood cells were removed, and the peripheral blood lymphocytes were washed and suspended in RPMI-1640 medium supplemented with 10% heat-inactivated fetal bovine serum, 100 U/mL penicillin and 100 μg/mL streptomycin. Then, the cells were cultured for 5 h in the presence of phorbol 12-myristate 13-acetate (PMA) (50 ng/ml;Solarbio),ionomycin calcium salt (1 μg/ml; Solarbio) and GolgiStop (0.67 μl/ml)

来源文献:Xu X, Feng X, Wang L, Yi T, Zheng L, Jiang G, Fan S, Liao Y, Feng M, Zhang Y, Li D, Li Q. A HSV1 mutant leads to an attenuated phenotype and induces immunity with a protective effect. PLoS Pathog. 2020 Aug 10;16(8):e1008703. doi: 10.1371/journal.ppat.1008703. PMID: 32776994; PMCID: PMC7440667.


Cell(THP-1 cell,200 ng/mL PMA,24h

THP-1 cells (1.5×105 cells/ml) were treated with 200 ng/ml phorbol myristate acetate (PMA, Solarbio Science & Technology Co., Ltd, Beijing, China.) for 24 h and polarized into macrophages. To induce TAMs, THP-1 macrophages were cultured with CM of GC cells for a further 24h prior to harvesting.

来源文献:Sun L ,  Li J ,  Yan W , et al. H19 contributes to aerobic glycolysis, proliferation and immune escape of gastric cancer cells via the miR-519d-3p/LDHA axis.  2020.


Cell(THP-1 AR silencing and differentiation,50 ng/mL PMA,24h

THP-1 cells were infected for 24 h with lentiviruses harboring (AR scramble or AR shRNA) shRNAs that target human AR and cloned into the U6-shRNA-EF1α- EGFP-Puro vector. The U6-shRNA-EF1α- EGFP-Puro vector encoding a scrambled sequence not matching any mammalian sequence was used as control. Positively infected cells were sorted by flow cytometry 72 h after infection. Infected THP-1 monocytes (THP-1sc and THP-1ARsi) were induced to differentiate into macrophages (MФsc and MФARsi) by exposing to 50 ng/mL phorbol myristate acetate (PMA) (Solarbio, Beijing, China) for 24 h, then incubated for an additional 24 h in the absence of PMA in a conditioning medium (CM). The collected CM was used to treat HASMCs for investigating the role of AR in HASMC calcification.

来源文献:Pang H, Xiao L, Lu Z, Chen H, Shang Z, Jiang N, Wang X, Wei F, Jiang A, Chen Y, Niu Y. Targeting androgen receptor in macrophages inhibits phosphate-induced vascular smooth muscle cell calcification by decreasing IL-6 expression. Vascul Pharmacol. 2020 Jul;130:106681. doi: 10.1016/j.vph.2020.106681. Epub 2020 May 5. PMID: 32387336.


Cell(HepG2 cells,10 nmol/L PMA,1h,37 °C

To monitor endogenous ClO−, HepG2 cells were treated with LPS(1 μg/mL) for 12 h and then coincubated with PMA (10 nmol/L) and the peptide@Ag/Au NCs solution at 37 °C for 1 h, followed by washing three times before imaging. In the control assay, HepG2 cells were treated with LPS (1 μg/mL) for 12 h and then incubated with PMA (10 nmol/L) for 1 h. The cells were subsequently cultured in medium containing uric acid (250 nmol/L) and DMSO (0.5%) for 15 min and then treated with the peptide@Ag/Au NCs solution at 37 °C for 1 h. The cells were washed three times with PBS to remove the unbound peptide@Ag/Au NCs and were then observed under a Nikon A1R MP multiphoton microscope with a 60× oil-immersion objective lens. The images of peptide@Ag/Au NCs were captured under excitation at409 nm.

来源文献:Jia M, Mi W, Guo S, Yang QZ, Jin Y, Shao N. Peptide-capped functionalized Ag/Au bimetal nanoclusters with enhanced red fluorescence for lysosome-targeted imaging of hypochlorite in living cells. Talanta. 2020 Aug 15;216:120926. doi: 10.1016/j.talanta.2020.120926. Epub 2020 Mar 14. PMID: 32456892.



备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.

实验图
暂无详情
Chen Z, et al. Bioact Mater. 2020 Aug 7;6(1):1-11.
Jia M,et al. Talanta. 2020 Aug 15;216:120926.
Wu Y,et al. Talanta. 2021 Jan 15;222:121449.
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