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二磷酸核酮糖羧化酶/加氧酶(Rubisco)活性检测试剂盒
检测方法:紫外分光光度法 Spectrophotometer
英文名称:Rubisco-Bisphosphate Carboxylase/Oxygenase (Rubisco) Activity Assay Kit
储存条件:Store at -20℃，6 months
产品简介
| 中文名称 | 二磷酸核酮糖羧化酶/加氧酶(Rubisco)活性检测试剂盒 |
| 英文名称 | Rubisco-Bisphosphate Carboxylase/Oxygenase (Rubisco) Activity Assay Kit |
| 检测方法 | 紫外分光光度法 Spectrophotometer |
| 储存条件 | Store at -20℃，6 months |
| 规格 | 25T/24S 50T/48S |
| 单位 | 盒 |
核酮糖-1,5-二磷酸核酮糖羧化/加氧酶（Rubisco, EC 4.1.1.39）是植物光合作用中的一个关键酶，既控制着CO2的固定，同时又制约着碳素向Calvin循环和光呼吸循环分流，其活性的大小直接影响着光合速率。
测定原理：
（1）在Rubisco 的催化下，1 分子的核酮糖-1，5-二磷酸（RuBP）与1 分子的CO2 结合，产生2 分子的3-磷酸甘油酸（PGA）；
（2）PGA 可通过外加的3-磷酸甘油酸激酶和甘油醛-3-磷酸脱氢酶的作用，产生甘油醛-3-磷酸，伴随着NADH 氧化生成NAD+；
（3）在340 nm NADH有特征吸收峰，而NAD+没有此吸收峰，因此测定340nm 吸光度下降速率可以代表Rubisco的羧化酶活性。
需自备的仪器和用品：
可见-紫外分光光度计、台式离心机、水浴锅、可调式移液器、1mL 石英比色皿、研钵、冰和蒸馏水。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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