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异硫氰酸荧光素酯

CAS:No.3326-32-7

英文名称:FITC

分子式:C21H11NO5S

分子量:389.38

储存条件:Powder:2-8℃,2 years;Insolvent(母液):-20℃,6 months;-80℃,1 year

纯度:HPLC≥97%

异硫氰酸荧光素酯
满3999赠露营车
货号:
IF0080
品牌:
Solarbio
SKU 北京总部 北京海淀 武汉 广州
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使用本产品的案例(仅供参考

In Vitro


Phagocytosis Assay of Hemocyte(血细胞吞噬实验,FITC-labeled C. albicans (1×105 cells, 5 µl),37◦C for 3 h in dark

In brief, 15 larvae per group were inoculated with 5 µl of PBS or ZnO-NP suspensions (2 mg/kg) via the last left proleg. After incubation at 37◦C for 2 h, each larva was challenged with fluorescein isothiocyanate isomer I (FITC; Solarbio, Beijing, China)-labeled C. albicans (1×105 cells, 5 µl) and incubated at 37◦C for 3 h in dark.    For preparing FITC-labeled C. albicans, C. albicans suspensions (1 × 109 cells/ml) were incubated with 0.1 mg/ml in a shaking incubator in the dark for 1 h and washed with PBS three times.    Subsequently, 50 µl of hemolymph was obtained from each larva and diluted in a 12-well plate containing 950 µl of IPS and 0.002% PTU. The plate was gently shaken followed by standing for 15 min at room temperature. The phagocytosis of C. albicans by hemocytes was visualized using an IX73 fluorescence microscope.

来源文献:Xu MN, Li L, Pan W, Zheng HX, Wang ML, Peng XM, Dai SQ, Tang YM, Zeng K, Huang XW. Zinc Oxide Nanoparticles Prime a Protective Immune Response in Galleria mellonella to Defend Against Candida albicans. Front Microbiol. 2021 Dec 10;12:766138. doi: 10.3389/fmicb.2021.766138. PMID: 34956129; PMCID: PMC8702860.


Cells流式细胞术MDA-MB-231/RT-R and BT-549/RT-R cells20 µg/mL

After 8 Gy γ-irradiation, the cells were detached by trypsin and stained with 20 µg/mL of Annexin V-fluorescein isothiocyanate (FITC) (IF0080, Solarbio) and 50 µg/mL of propidium iodide (PI, P8080, Solarbio), while flow cytometry was performed to analyze the change in apoptosis rate.

文献来源:Li Y, Liang L. Transcription Impairment of TMEM208 by ZBTB14 Suppresses Breast cancer Radiotherapy Resistance. J Mammary Gland Biol Neoplasia. 2024 Dec 18;29(1):20. doi: 10.1007/s10911-024-09573-1. PMID: 39692812; PMCID: PMC11655580.



备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.

实验图
Wang M , Bi S , Pang J ,et al.Precise quantification of the antibacterial activity of chitosan by NB medium neutralizer,2021.
暂无详情
Xu MN, et al. Front Microbiol. 2021 Dec 10;12766138.
Li Y,et al.J Mammary Gland Biol Neoplasia. 2024 Dec 18;29(1):20. doi: 10.1007/s10911-024-09573-1.
注意:
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响,相关处理只限于产品本身,不涉及其他赔偿。
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