异硫氰酸荧光素酯
CAS:No.3326-32-7
英文名称:FITC
分子式:C21H11NO5S
分子量:389.38
储存条件:Powder:2-8℃,2 years;Insolvent(母液):-20℃,6 months;-80℃,1 year
纯度:HPLC≥97%
产品简介
CAS | No.3326-32-7 |
中文名称 | 异硫氰酸荧光素酯 |
英文名称 | FITC |
别名 | Fluorescein 5-isothiocyanate |
分子式 | C21H11NO5S |
分子量 | 389.38 |
溶解性 | Soluble in DMSO ≥10mg/mL;Soluble in Ethanol ≥1mg/mL;Soluble in Water <1mg/mL |
纯度 | HPLC≥97% |
外观(性状) | Light yellow to orange yellow Solid |
储存条件 | Powder:2-8℃,2 years;Insolvent(母液):-20℃,6 months;-80℃,1 year |
EC | EINECS 248-207-7 |
MDL | MFCD00005063 |
SMILES | O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=C(N=C=S)C=C5 |
InChIKey | MHMNJMPURVTYEJ-UHFFFAOYSA-N |
InChI | InChI=1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H |
PubChem CID | 18730 |
背景说明 | FITC是荧光素的衍生物,用于标记胺。Ex=488nm,Em=525nm。 |
生物活性 | FITC是荧光素的衍生物,用于标记胺[1-2]。 |
In Vitro | Annexin V-FITC/PI用于检测软骨细胞凋亡[1]。 PEI修饰的CD与异硫氰酸荧光素(FITC)缀合以制备CDs-FITC复合物[2]。 |
细胞实验 | 用膜联蛋白V-荧光素异硫氰酸酯(FITC)和碘化丙啶通过流式细胞术测量软骨细胞凋亡的频率。从每组中收集总共1×104个处理的软骨细胞,在冷PBS中洗涤并在室温下与膜联蛋白V-FITC和PI在黑暗中在冰上孵育15分钟。然后使用荧光激活细胞分选仪分析这些样品。 Cell Quest软件用于分析细胞凋亡的百分比。所有测试重复三次。 |
数据来源文献 |
[1]. Gao SG, et al. Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes. Exp Ther Med. 2016 Nov;12(5):3488-3494. Epub 2016 Oct 5. [2]. Zhu X, et al. Ratiometric, visual, dual-signal fluorescent sensing and imaging of pH/copper ions in real samples based on carbon dots-fluorescein isothiocyanate composites. Talanta. 2017 Jan 1;162:65-71 |
规格 | 100mg 10mM*1mL in DMSO 500mg |
单位 | 瓶 |
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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Preparation of Corn Peptides with Anti-Adhesive Activity and Its Functionality to Alleviate Gastric Injury Induced by Helicobacter pylori Infection In Vivo
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Author:
Guanlong Li, Xiaolan Liu, Zhengfei Miao, Nan Hu, Xiqun Zheng
IF:
5.9000
Publish_to:
Nutrients
PMID:
37571404
Milk Exosomes Facilitate Oral Delivery of Drugs against Intestinal Bacterial Infections
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Author:
Shaoqi Qu, Yiming Han, Ying Liu, Jiajia Zhu, Ulas Acaroz, Jianzhong Shen, Kui Zhu
IF:
5.8950
Publish_to:
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
PMID:
36515136
Multicomponent thermosensitive lipid complexes enhance desmoplastic tumor therapy through boosting anti-angiogenesis and synergistic strategy
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Author:
Jian Guo, Huating Zeng, Yimin Liu, Xinmeng Shi, Yuping Liu, Congyan Liu, Yan Chen
IF:
5.8750
Publish_to:
INTERNATIONAL JOURNAL OF PHARMACEUTICS
PMID:
33781886
Encapsulation of Selenium Nanoparticles and Metformin in Macrophage-Derived Cell Membranes for the Treatment of Spinal Cord Injury
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Author:
Xiaobang Liu, Junpeng Sun, Jiaqun Du, Jinyu An, Yingqiao Li, Yu Hu, Ying Xiong, Yanan Yu, He Tian, Xifan Mei, Chao Wu
IF:
5.8000
Publish_to:
ACS Biomaterials Science & Engineering
PMID:
37713674
An extracellular matrix (ECM)-mimetic coating with dual bionics for Cardiovascular Stents
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Author:
Chen Nuoya, Li Mingyu, Wu Haoshaung, Qin Yumei, Wang Jian, Xu Kai, Luo Rifang, Yang Li, Wang Yunbing, Zhang Xingdong
IF:
5.7630
Publish_to:
Regenerative Biomaterials
PMID:
37359731
Flower-like Composite Material Delivery of Co-Packaged Lenvatinib and Bufalin Prevents the Migration and Invasion of Cholangiocarcinoma
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Author:
Zhouyu Ning, Yingke Zhao, Xia Yan, Yongqiang Hua, Zhiqiang Meng
IF:
5.7190
Publish_to:
Nanomaterials
PMID:
35745387
Redox-Responsive Nanopesticides Based on Natural Polymers for Environmentally Safe Delivery of Pesticides with Enhanced Foliar Dispersion and Washout Resistance
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Author:
Yingjian Ma, Junyao Li, Qinshu Yang, Fengyu Li, Lei Wang, Pengkun Yan, Xinyu Guo, Rui Zhao, Yucheng Gu, Yong Xu, Xuemin Wu
IF:
5.7000
Publish_to:
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
PMID:
39226432
Zinc Oxide Nanoparticles Prime a Protective Immune Response in Galleria mellonella to Defend Against Candida albicans
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Author:
Xu Mei-nian, Li Li, Pan Wen, Zheng Huan-xin, Wang Meng-lei, Peng Xiao-ming, Dai Si-qi, Tang Ying-mei, Zeng Kang, Huang Xiao-wen
IF:
5.6400
Publish_to:
Frontiers in Microbiology
PMID:
34956129
Efficiency of a novel thermosensitive enema in situ hydrogel carrying Periplaneta americana extracts for the treatment of ulcerative colitis
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Author:
Ming Wu, Hui Ding, Xiao Tang, Jiayi Chen, Meng Zhang, Ziqiong Yang, Qian Du, Jun Wang
IF:
5.6000
Publish_to:
Frontiers in Pharmacology
PMID:
36843930
Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from Lactobacillus Strains
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Author:
Qianqian Lu, Yingqi Guo, Guo Yang, Lei Cui, Zhen Wu, Xiaoqun Zeng, Daodong Pan, Zhendong Cai
IF:
5.5610
Publish_to:
Foods
PMID:
35681360
Development and characterization of MAO/PLA-nHA nanocomposite coatings on pure zinc for orthopedic applications
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Author:
Quanxin Chen, Xinglong Zhu, Yongqi Jiang, Lijing Yang, Huinan Hannah Liu, Zhenlun Song
IF:
5.4000
Publish_to:
SURFACE & COATINGS TECHNOLOGY
PMID:
Novel double-layered PLGA microparticles-dissolving microneedle (MPs-DMN) system for peptide drugs sustained release by transdermal delivery
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Author:
Bo Xu, Han Liu, Guozhong Yang, Suohui Zhang, Zequan Zhou, Yunhua Gao
IF:
5.3000
Publish_to:
INTERNATIONAL JOURNAL OF PHARMACEUTICS
PMID:
39722375
Properties of Heat-Assisted pH Shifting and Compounded Chitosan from Insoluble Rice Peptide Precipitate and Its Application in the Curcumin-Loaded Pickering Emulsions
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Author:
Zhenyu Yang, Zhiying Li, Zitong Xu, Zhihao Kong, Xin Qiao, Liwen Zhang, Lei Dai, Yanfei Wang, Qingjie Sun, David Julian McClements, Xingfeng Xu
IF:
5.2000
Publish_to:
Foods
PMID:
38137189
CRP Involved in Nile Tilapia (Oreochromis niloticus) against Bacterial Infection
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Author:
Qi Li, Baijian Jiang, Zhiqiang Zhang, Yongxiong Huang, Zhou Xu, Xinjin Chen, Jia Cai, Yu Huang, Jichang Jian
IF:
5.1680
Publish_to:
Biology-Basel
PMID:
36009776
Marine bacterial exopolysaccharide EPS11 inhibits migration and invasion of liver cancer cells by directly targeting collagen I
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Author:
Ge Liu, Rui Liu, Yeqi Shan, Chaomin Sun
IF:
5.1570
Publish_to:
JOURNAL OF BIOLOGICAL CHEMISTRY
PMID:
34461092
Four novel anti-adhesive activity peptides against Helicobacter pylori derived from rice bran protein: release, identification and anti-adhesive mechanisms elucidation
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Author:
Guanlong Li, Zhengfei Miao, Xiaolan Liu, QuanXin Wang, Xiqun Zheng
IF:
5.1000
Publish_to:
Food & Function
PMID:
39042096
EDTA-functionalized silica nanoparticles as a conditioning agent for dentin bonding using etch-and-rinse technique
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Author:
Jianan Yu, Yuexiang Li, Xinyuan Liu, Haowen Huang, Yu Wang, Qunlin Zhang, Quanli Li, Chris Ying Cao
IF:
4.9910
Publish_to:
JOURNAL OF DENTISTRY
PMID:
37105434
A new self-immolative colistin prodrug with dual targeting functionalities and reduced toxicity for the treatment of intracellular bacterial infections
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Author:
Gengqi Liu, Di Lu, Shiyu Zhu, Minchao Hao, Xingyue Yang, Xiaojian Wang, Yumiao Zhang
IF:
4.8540
Publish_to:
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
PMID:
35593460
Molecular characterization and functional analysis of galectin-1 from silver pomfret (Pampus argenteus)
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Author:
Suyang Chen, Tingting Gao, Xionglin Li, Kejing Huang, Lu Yuan, Suming Zhou, Jianhu Jiang, Yajun Wang, Jiasong Xie
IF:
4.7000
Publish_to:
FISH & SHELLFISH IMMUNOLOGY
PMID:
37944682
CpG ODN 2102 promotes antibacterial immune responses and enhances vaccine-induced protection in golden pompano (Trachinotus ovatus)
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Author:
Xiaojuan Chen, Ying Wu, Yulin Qiu, Pengshuo Li, Zhenjie Cao, Yongcan Zhou, Yun Sun
IF:
4.6220
Publish_to:
FISH & SHELLFISH IMMUNOLOGY
PMID:
37137380
Identification and characterization of a C-type lectin in turbot (Scophthalmus maximus) which functioning as a pattern recognition receptor that binds and agglutinates various bacteria
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Author:
Qing Zhu, Huijun Huo, Qiang Fu, Ning Yang, Ting Xue, Changju Zhuang, Xiaoli Liu, Beibei Wang, Baofeng Su, Chao Li
IF:
4.5810
Publish_to:
FISH & SHELLFISH IMMUNOLOGY
PMID:
34062237
Effect of Platelet-Rich Plasma Addition on the Chemical Properties and Biological Activity of Calcium Sulfate Hemihydrate Bone Cement
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Author:
Jingyu Liu, Yifan Wang, Yanqin Liang, Shengli Zhu, Hui Jiang, Shuilin Wu, Xiang Ge, Zhaoyang Li
IF:
4.5000
Publish_to:
Biomimetics
PMID:
37366857
The chiisanoside derivatives present in the leaves of Acanthopanax sessiliflorus activate autophagy through the LRP6/GSK3β axis and thereafter inhibit oxidative stress, thereby counteracting cisplatin-induced ototoxicity
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Author:
Wenxin Zhang, Hongbo Teng, Tianyi Zhao, Roberts I. Eglitis, Xv Wang, Zhengxuan Yu, Shurong Qu, Haijing Wang, Yaru Zhao, Bowen Fan, Shuangli Liu, Yan Zhao
IF:
4.4000
Publish_to:
Frontiers in Pharmacology
PMID:
39881873
The Amelogenin-Derived Peptide TVH-19 Promotes Dentinal Tubule Occlusion and Mineralization
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Author:
Xiu Peng, Sili Han, Kun Wang, Longjiang Ding, Zhenqi Liu, Linglin Zhang
IF:
4.3290
Publish_to:
Polymers
PMID:
34372076
Tumoral EIF4EBP1 regulates the crosstalk between tumor-associated macrophages and tumor cells in MRTK
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Author:
Xin Wu, Tao Mi, Liming Jin, Chunnian Ren, Jinkui Wang, Zhaoxia Zhang, Jiayan Liu, Zhaoyin Wang, Peng Guo, Dawei He
IF:
4.2000
Publish_to:
EUROPEAN JOURNAL OF PHARMACOLOGY
PMID:
38944176
Enhancement of immune responses using ovalbumin-conjugated Eucommia ulmoides leaf polysaccharides encapsulated in a cubic liquid-crystalline phase delivery system
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Author:
Haibo Feng, Linzi Zhang, Jie Yang, Sheng Li, Feng Tang, Hangyu Li, Xinnan Zhang, Daiyan Wu, Yangyang Feng, Qianqian Liu, Ziwei Liu
IF:
4.1250
Publish_to:
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
PMID:
35638143
Bacterial recognition and inhibition activities of an LRR-only protein in the Chinese mitten crab, Eriocheir sinensis
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Author:
Yi Zhang, Yunhui Bai, Jinbin Zheng, Zhaoxia Cui
IF:
4.1000
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FISH & SHELLFISH IMMUNOLOGY
PMID:
39098748
Discovery of Salifungin as a Repurposed Antibiotic against Methicillin-Resistant Staphylococcus aureus with Limited Resistance Development
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Author:
Chenchen Wang, Yueyue Ji, Xingyu Huo, Xiaodan Li, Wenjia Lu, Zhaoran Zhang, Wenqi Dong, Xiangru Wang, Huanchun Chen, Chen Tan
IF:
4.0000
Publish_to:
ACS Infectious Diseases
PMID:
38581387
Identification and response analysis of xyloglucan endotransglycosylase/hydrolases (XTH) family to fluoride and aluminum treatment in Camellia sinensis
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Author:
Wu Zichen, Cui Chuanlei, Xing Anqi, Xu Xiaohan, Sun Yi, Tian Zhiqiang, Li Xuyan, Zhu Jiangyuan, Wang Genmei, Wang Yuhua
IF:
3.9690
Publish_to:
BMC GENOMICS
PMID:
34696727
GelMA/κ-carrageenan double-network hydrogels with superior mechanics and biocompatibility
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Author:
Xueqi Gan, Chen Li, Jiyu Sun, Xidan Zhang, Min Zhou, Yi Deng, Anqi Xiao
IF:
3.9000
Publish_to:
RSC Advances
PMID:
36688070
3D MXene microspheres with honeycomb architecture for tumor photothermal/photodynamic/chemo combination therapy
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Author:
Yaping Guo, Hongshui Wang, Xin Feng, Yunbo Zhao, Chunyong Liang, Lei Yang, Mingjun Li, Yongguang Zhang, Wei Gao
IF:
3.8740
Publish_to:
NANOTECHNOLOGY
PMID:
33513589
Effect of microencapsulated Fiber2-displaying probiotics loaded with inulin nanoparticles on immunity against fowl adenovirus serotype 4 in chickens
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Author:
Biao Wang, Meiyuan Tian, Yashu Yang, Zhipeng Jia, Faisal R Anjum, Chunli Ma, Dexing Ma
IF:
3.8000
Publish_to:
POULTRY SCIENCE
PMID:
39778367
MXene Quantum Dot/Zeolitic Imidazolate Framework Nanocarriers for Dual Stimulus Triggered Tumor Chemo-Phototherapy
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Author:
Xin Feng, Mingjun Li, Jianming Wang, Xianrui Zou, Hongshui Wang, Donghui Wang, Huan Zhou, Lei Yang, Wei Gao, Chunyong Liang
IF:
3.7480
Publish_to:
Materials
PMID:
35806667
Pseudorabies virus infection increases the permeability of the mammalian respiratory barrier to facilitate Pasteurella multocida infection
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Author:
Dajun Zhang, Lin Lin, Jie Yang, Qingjie Lv, Mixue Wang, Lin Hua, Keshan Zhang, Huanchun Chen, Bin Wu, Zhong Peng
IF:
3.7000
Publish_to:
mSphere
PMID:
39041808
Molecular characterization and antibacterial ability of galectin-3 and galectin-9 in Onychostoma macrolepis
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Author:
Hongzhou Xu, Haixia Liu, Chengrong Liu, Xinyan Shangguan, Xu Cheng, Ruifang Zhang, Yitong Lu, Ping Li, Yingjie Cai
IF:
3.6360
Publish_to:
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
PMID:
34914929
pH-responsive black phosphorus quantum dots for tumor-targeted photodynamic therapy
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Author:
Zhaoyuan Liu, Zhongjian Xie, Xinqiang Wu, Zijian Chen, Wenting Li, Xiaofeng Jiang, Liangqi Cao, Dawei Zhang, Qiwen Wang, Ping Xue, Han Zhang
IF:
3.6310
Publish_to:
Photodiagnosis and Photodynamic Therapy
PMID:
34237475
IVT cell-free biosensors for tetracycline and macrolide detection based on allosteric transcription factors (aTFs)
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Author:
Huaixiu Bi, Chen Zhao, Yongkang Zhang, Xi Zhang, Bin Xue, Chenyu Li, Shang Wang, Xiaobo Yang, Chao Li, Zhigang Qiu, Jingfeng Wang, Zhiqiang Shen
IF:
3.5320
Publish_to:
Analytical Methods
PMID:
36314439
Biomimetic Inorganic Nanoparticle-Loaded Silk Fibroin-Based Coating with Enhanced Antibacterial and Osteogenic Abilities
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Author:
Yunpeng Zhang, Xiaorong Chen, Yuan Li, Tian Bai, Chen Li, Lingyan Jiang, Yu Liu, Changying Sun, Wenhao Zhou
IF:
3.5120
Publish_to:
ACS Omega
PMID:
34778674
The role of a novel secretory peptidoglycan recognition protein with antibacterial ability from the Chinese Oak Silkworm Antheraea pernyi in humoral immunity
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Author:
Xutong Duan, Ting Fu, Chang Liu, Fuhui Wang, Chengbao Liu, Lin Zhao, JinZhu Yu, Xialu Wang, Rong Zhang
IF:
3.2000
Publish_to:
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
PMID:
38880307
Transcription Impairment of TMEM208 by ZBTB14 Suppresses Breast cancer Radiotherapy Resistance
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Author:
Li Yan, Liang Lili
IF:
3.0000
Publish_to:
JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA
PMID:
39692812
Inhibitory role of microRNA-484 in kidney stone formation by repressing calcium oxalate crystallization via a VDR/FoxO1 regulator axis
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Author:
Fan Li, Li Hai, Huo Wei
IF:
2.8610
Publish_to:
Urolithiasis
PMID:
36227295
Nerve growth factor loaded macrophage-derived nanovesicles for inhibiting neuronal apoptosis after spinal cord injury
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Author:
Nan Xia, Zhanshan Gao, Hengshuo Hu, Daoyong Li, Chuanjie Zhang, Xifan Mei, Chao Wu
IF:
2.6460
Publish_to:
JOURNAL OF BIOMATERIALS APPLICATIONS
PMID:
34167336
Assessment of probiotic adhesion and inhibitory effect on Escherichia coli and Salmonella adhesion
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Author:
Wang Ya, Jia Dan, Wang Jia-hui, Li He-hai, Liu Jun-long, Liu Ai-hong, Wang Jin-ming, Guan Gui-quan, Luo Jian-xun, Yin Hong, Li You-quan
IF:
2.5520
Publish_to:
ARCHIVES OF MICROBIOLOGY
PMID:
34581832
Lactobacillus animalis pZL8a: a potential probiotic isolated from pig feces for further research
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Author:
Jia Dan, Wang Ya, Wang Jiahui, Liu Junlong, Li Hehai, Liu Aihong, Wang Jinming, Guan Guiquan, Luo Jianxun, Yin Hong, Li Youquan
IF:
2.4060
Publish_to:
3 Biotech
PMID:
33680697
Bacterial coloration immunofluorescence strip for ultrasensitive rapid detection of bacterial antibodies and targeted antibody-secreting hybridomas
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Author:
Shuiqin Fang, Hao Yang, Cheng Liu, Yachen Tian, Meijiao Wu, Youxue Wu, Qing Liu
IF:
2.3030
Publish_to:
JOURNAL OF IMMUNOLOGICAL METHODS
PMID:
34933017
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