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大鼠肿瘤坏死因子α检测试剂盒
英文名称:Rat TNF-α ELISA Kit
应用: Sandwich ELISA,ELISA
反应性:Rat
样品类型:血清/血浆/细胞培养上清
灵敏度:15 pg/ml
储存条件:2-8℃，6 months
产品简介
| 中文名称 | 大鼠肿瘤坏死因子α检测试剂盒 |
| 英文名称 | Rat TNF-α ELISA Kit |
| 别名 | APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha |
| 储存条件 | 2-8℃，6 months |
| 检测目标 | TNF-α |
| 样品类型 | 血清/血浆/细胞培养上清 |
| 反应性 | Rat |
| 反应类型 | Sandwich-ELISA |
| 定量/定性 | 定量检测 |
| 检测范围 | 31.25-2000 pg/ml |
| 灵敏度 | 15 pg/ml |
| 特异性 | 不与人TNF-α,TNF-β，猪TNF-α，小鼠TNF-α等反应。 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 反应时间 | 3.5h |
| Swiss Prot | P16599 |
| Gene ID | 24835 |
| 试剂盒类型 | 传统ELISA试剂盒 |
| 检测原理 | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠 TNF-α单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的大鼠 TNF-α会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗大鼠 TNF-α抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠 TNF-α发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的大鼠 TNF-α，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的大鼠 TNF-α浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠 TNF-α的浓度。 |
| 规格 | 48T 96T |
| 应用 | Sandwich ELISA ELISA |
| 疾病系列 | 肥胖研究相关因子 骨质疏松症研究相关因子 冠心病研究相关因子 慢阻肺COPD研究相关因子 骨关节炎相关因子 神经系统疾病相关因子 糖尿病研究相关因子 系统性红斑狼疮相关因子 哮喘相关因子 心血管疾病相关因子 |
| 研究领域 | 心血管 新陈代谢 微生物 细胞生物学 信号传导 肿瘤&癌症 免疫学 |
| 单位 | 盒 |
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
实验图
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Quercetin influences intestinal dysbacteriosis and delays alveolar epithelial cell senescence by regulating PTEN/PI3K/AKT signaling in pulmonary fibrosis
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Author：
Wu Wenjuan, Wu Xinhui, Qiu Lingxiao, Wan Ruijie, Zhu Xiaoming, Chen Song, Yang Xinying, Liu Xueya, Wu Jizhen
IF：
3.6000
Publish_to：
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
PMID：
38153514
Maresin1 Ameliorates Sepsis-Induced Microglial Neuritis Induced through Blocking TLR4-NF-κ B-NLRP3 Signaling Pathway
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Author：
Huiping Wu, Ying Wang, Haiyan Fu, Lili Ji, Na Li, Dan Zhang, Longxiang Su, Zhansheng Hu
IF：
3.5080
Publish_to：
Journal of Personalized Medicine
PMID：
36983716
The mechanism of Traditional Mongolian medicine Daruqi particles on inflammation
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Author：
Zhi Liu, Zhiqiang Han, Lan Xue, Wei Wei, Batudeligen
IF：
3.5000
Publish_to：
GENE
PMID：
38703870
Exosomes Derived from lncRNA TCTN2-Modified Mesenchymal Stem Cells Improve Spinal Cord Injury by miR-329-3p/IGF1R Axis
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Author：
Liu Jian, Lin Mingxia, Qiao Feng, Zhang Chenghua
IF：
3.4440
Publish_to：
JOURNAL OF MOLECULAR NEUROSCIENCE
PMID：
34623606
Overexpression of SESN1 improves mitochondrial damage and mitophagy, a potential therapeutic strategy for cognitive dysfunction after anaesthesia
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Author：
Li Sun, Xiaoya Hong, Daliang Wang, Yangyang Li
IF：
3.4000
Publish_to：
EUROPEAN JOURNAL OF NEUROSCIENCE
PMID：
38105520
Hydroxychloroquine ameliorates immune functionality and intestinal flora disorders of IgA nephropathy by inhibition of C1GALT1/Cosmc pathway
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Author：
Chaochao Wang, Xiaoqiao Cai, Shengfen Lin, Yongqiang Lin
IF：
3.3000
Publish_to：
IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
PMID：
38151955
The therapeutic effect of different cumin essential oil fractions against gastric ulcer in rats
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Author：
Shuai Lu, Feiya Suo, Wenfeng Yu, Guofeng Wu
IF：
3.2000
Publish_to：
JOURNAL OF FOOD SCIENCE
PMID：
39690115
The targeting of YAP by kaempferol regulates bone homeostasis and improves osteoporosis in rats
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Author：
Liu Wencheng, Liu Rifu, Yang Yue, Cheng Lixue, Feng Xiaobing, Li Qinglei
IF：
3.1000
Publish_to：
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
PMID：
40208316
Ginkgetin Alleviates Intervertebral Disc Degeneration by Inhibiting Apoptosis,Inflammation,and Disturbance of Extracellular Matrix Synthesis and Catabolism via Inactivation of NLRP3 Inflammasome
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Author：
Baoshan Hu, Shan Lin, Shengrong Lin, Gang Rui
IF：
3.0440
Publish_to：
IMMUNOLOGICAL INVESTIGATIONS
PMID：
37154418
Baicalein, a component of banxia xiexin decoction, alleviates CPT-11-induced gastrointestinal dysfunction by inhibiting ALOX15-mediated ferroptosis
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Author：
Jingbo Pei, Yuanyuan Zou, Wenying Zhou, Yakun Wang
IF：
3.0000
Publish_to：
Chemical Biology & Drug Design
PMID：
37735740
Bone marrow mesenchymal stem cells-derived exosomes reduce apoptosis and inflammatory response during spinal cord injury by inhibiting the TLR4/MyD88/NF-κB signaling pathway
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Author：
Liying Fan, Jun Dong, Xijing He, Chun Zhang, Ting Zhang
IF：
2.9030
Publish_to：
HUMAN & EXPERIMENTAL TOXICOLOGY
PMID：
33779331
Protective effects of peanut skin extract on high-fat and high-fructose diet-induced kidney injury in rats
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Author：
Yang Yang, Yu Jing, Huo Jiaoyao, Yang Luting, Yan Yaping
IF：
2.9000
Publish_to：
FOOD SCIENCE AND BIOTECHNOLOGY
PMID：
37215259
Quercetin Alleviates Neuropathic Pain in the Rat CCI Model by Mediating AMPK/MAPK Pathway
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Author：
Guangyao Ye, Chunyan Lin, Yu Zhang, Zihan Ma, Yuebo Chen, Lingsi Kong, Liyong Yuan, Tao Ma
IF：
2.8320
Publish_to：
Journal of Pain Research
PMID：
34040433
A novel NAC transcription factor, PpNAP6, is involved in peach ripening by activating ethylene synthesis
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Author：
Zuo Ting, Gong Bingzheng, Gao Yang, Yuan Liangjie
IF：
2.8000
Publish_to：
MOLECULAR BIOLOGY REPORTS
PMID：
37344642
Chemerin promotes MAPK/ERK activation to induce inflammatory factor production in rat synoviocytes
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Author：
Chen Wang, Songrui Zhang, Longao Huang, Jianyi Liu, Quan Zhou, Gang Du, Shan Lao
IF：
2.7510
Publish_to：
Experimental and Therapeutic Medicine
PMID：
36277163
Fecal Microbiota Transplantation Alleviated Paclitaxel-Induced Peripheral Neuropathy by Interfering with Astrocytes and TLR4/p38MAPK Pathway in Rats
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Author：
Haibin Shi, Minmin Chen, Caihong Zheng, Bian Yinglin, Bin Zhu
IF：
2.7000
Publish_to：
Journal of Pain Research
PMID：
37483406
Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating H2S/CSE and NO/nNOS Systems
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Author：
Ying Liu, Wenhua Wei, Shiwei Liang, Haicheng Fang, Jie Cao
IF：
2.6500
Publish_to：
Evidence-based Complementary and Alternative Medicine
PMID：
36091585
miR-132-3p participates in the pathological mechanism of temporomandibular joint osteoarthritis by targeting PTEN
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Author：
Libo Zhou, Deping Chen, Penghui Liu, Lei Chen, Yucheng Su
IF：
2.6400
Publish_to：
ARCHIVES OF ORAL BIOLOGY
PMID：
35878435
NF-κB affected the serum levels of TNF-α and IL-1β via activation of the MAPK/NF-κB signaling pathway in rat model of acute pulmonary microthromboembolism
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Author：
Yanfen Zhong, Binbin Liang, Xiaofeng Zhang, Jingtao Li, Decai Zeng, Tongtong Huang, Ji Wu
IF：
2.6000
Publish_to：
Pulmonary Circulation
PMID：
38584678
Remifentanil reduces multiple organ and energy metabolism disturbances in a rat sepsis model.
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Author：
Yang MX, Wu ZZ, Liao XY, Zhang BL, Chen X, Wu Y, Lin JD
IF：
2.5890
Publish_to：
JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
PMID：
35639040
The extract of concentrated growth factor enhances osteogenic activity of osteoblast through PI3K/AKT pathway and promotes bone regeneration in vivo
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Author：
Dong Kai, Zhou Wen-Juan, Liu Zhong-Hao, Hao Peng-Jie
IF：
2.3840
Publish_to：
International Journal of Implant Dentistry
PMID：
34345951
Toxicopathological, proinflammatory and stress response evaluation of Terminalia catappa extract in male Wistar rats
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Author：
Franklyn Nonso Iheagwam, Chiamaka Obiageli Okeke, Opeyemi Christianah De Campos, Bose Esther Adegboye, Olubanke Olujoke Ogunlana, Shalom Nwodo Chinedu
IF：
2.3000
Publish_to：
Toxicology Reports
PMID：
34712595
Mechanism of action of exercise regulating intestinal microflora to improve spontaneous hypertension in rats
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Author：
Yu Li, Xiaoju Song, Lianjing Dai, Yangyi Wang, Qiong Luo, Lei Lei, Yunfei Pu
IF：
2.2000
Publish_to：
Biomolecules and Biomedicine
PMID：
39484785
Ginsenoside Rg1 relieves rat intervertebral disc degeneration and inhibits IL-1β-induced nucleus pulposus cell apoptosis and inflammation via NF-κB signaling pathway
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Author：
Yu Lei, Hao Ying-Jie, Ren Zhi-Nan, Zhu Guang-Duo, Zhou Wei-Wei, Lian Xu, Wu Xue-Jian
IF：
2.1000
Publish_to：
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
PMID：
38485818
Effect of 3D printing technology-assisted TKA on cartilage tissue in rabbit with knee osteoarthritis.
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Author：
Wang G, Cheng F, Cui J, Chen N, Li Q
IF：
2.0000
Publish_to：
HISTOLOGY AND HISTOPATHOLOGY
PMID：
38639204
Esculetin attenuates cerebral ischemia-reperfusion injury and protects neurons through Nrf2 activation in rats
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Author：
Zhang Zhe, Zhang Jiayun, Shi Rui, Xu Tiantian, Wang Shiduo, Tian Junbiao
IF：
1.9000
Publish_to：
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
PMID：
39504067
MiR-210-3p Enhances Cardiomyocyte Apoptosis and Mitochondrial Dysfunction by Targeting the NDUFA4 Gene in Sepsis-Induced Myocardial Dysfunction
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Author：
Dandan Chen, Yu Hou, Xingjun Cai
IF：
1.8620
Publish_to：
International Heart Journal
PMID：
33994501
Naringin Prevents Diabetic-Induced Dysmetabolism in Male Wistar Rats by Modulating GSK-3 Activities and Oxidative Stress-Dependent Pathways
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Author：
Okesina Kazeem Bidemi, Odetayo Adeyemi Fatai, Adeyemi Wale Johnson, Okesina Akeem Ayodeji, Bassey Grace Edet, Olayaki Luqman Aribidesi
IF：
1.8000
Publish_to：
CELL BIOCHEMISTRY AND BIOPHYSICS
PMID：
39060916
Resveratrol inhibits multiple organ injury in preeclampsia rat model
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Author：
Xiao Lang, Wei Liu, Qinfang Chen, Xingyu Yang, Dan Chen, Weiwei Cheng
IF：
1.7000
Publish_to：
ACTA BIOCHIMICA POLONICA
PMID：
36735622
Tempol attenuates chronic intermittent hypoxia-induced lung injury through the miR-145-5p/Nrf2 signaling pathway
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Author：
Li Ai, Yongxia Li, Zhijuan Liu, Ran Li, Xiaona Wang, Bing Hai
IF：
1.6000
Publish_to：
CELLULAR AND MOLECULAR BIOLOGY
PMID：
38158661
Cinnamaldehyde induces apoptosis and enhances anti-colorectal cancer activity via covalent binding to HSPD1
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Author：
Qiaosu Xiao, Ting Li, Wenyang He
IF：
0.6000
Publish_to：
TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH
PMID：
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