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大鼠白细胞介素6检测试剂盒
英文名称:Rat IL-6 ELISA Kit
应用: Sandwich ELISA,ELISA
反应性:Rat
样品类型:血清/血浆/细胞培养上清
灵敏度:19.53 pg/ml
储存条件:2-8℃，6 months
产品简介
| 中文名称 | 大鼠白细胞介素6检测试剂盒 |
| 英文名称 | Rat IL-6 ELISA Kit |
| 别名 | B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; Interferon beta-2 |
| 储存条件 | 2-8℃，6 months |
| 检测目标 | IL-6 |
| 样品类型 | 血清/血浆/细胞培养上清 |
| 反应性 | Rat |
| 反应类型 | Sandwich-ELISA |
| 定量/定性 | 定量检测 |
| 检测范围 | 39.06-2500 pg/ml |
| 灵敏度 | 19.53 pg/ml |
| 特异性 | 不与大鼠GDNF、GM-CSF、IFN-γ、IL-1、IL-2、IL-4、IL-10、IL-18、PDGF-BB、TNF-α，人IL-6、IL-6 R等反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 反应时间 | 3.5h |
| Swiss Prot | P20607 |
| Gene ID | 24498 |
| 试剂盒类型 | 传统ELISA试剂盒 |
| 检测原理 | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠 IL-6单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的大鼠 IL-6会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗大鼠 IL-6抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠 IL-6发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的大鼠 IL-6，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的大鼠 IL-6浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠 IL-6的浓度 |
| 规格 | 48T 96T |
| 应用 | Sandwich ELISA ELISA |
| 疾病系列 | 肥胖研究相关因子 骨质疏松症研究相关因子 冠心病研究相关因子 慢阻肺COPD研究相关因子 骨关节炎相关因子 神经系统疾病相关因子 糖尿病研究相关因子 系统性红斑狼疮相关因子 哮喘相关因子 心血管疾病相关因子 |
| 研究领域 | 神经学 新陈代谢 肿瘤&癌症 免疫学 |
| 单位 | 盒 |
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
实验图
标准曲线图
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Ginkgetin Alleviates Intervertebral Disc Degeneration by Inhibiting Apoptosis,Inflammation,and Disturbance of Extracellular Matrix Synthesis and Catabolism via Inactivation of NLRP3 Inflammasome
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Author：
Baoshan Hu, Shan Lin, Shengrong Lin, Gang Rui
IF：
3.0440
Publish_to：
IMMUNOLOGICAL INVESTIGATIONS
PMID：
37154418
Baicalein, a component of banxia xiexin decoction, alleviates CPT-11-induced gastrointestinal dysfunction by inhibiting ALOX15-mediated ferroptosis
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Author：
Jingbo Pei, Yuanyuan Zou, Wenying Zhou, Yakun Wang
IF：
3.0000
Publish_to：
Chemical Biology & Drug Design
PMID：
37735740
Biotoxicity of paraquat to lung cells mediated by endoplasmic reticulum-mitochondria interaction
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Author：
Xiao Ping, Wu Shaohua, Wang Zhiyong, Shen Guoqiang, Shi Xiaofeng
IF：
2.9000
Publish_to：
JOURNAL OF MOLECULAR HISTOLOGY
PMID：
39215928
Quercetin Alleviates Neuropathic Pain in the Rat CCI Model by Mediating AMPK/MAPK Pathway
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Author：
Guangyao Ye, Chunyan Lin, Yu Zhang, Zihan Ma, Yuebo Chen, Lingsi Kong, Liyong Yuan, Tao Ma
IF：
2.8320
Publish_to：
Journal of Pain Research
PMID：
34040433
Molecular mechanism of paraquat-induced ferroptosis leading to pulmonary fibrosis mediated by Keap1/Nrf2 signaling pathway
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Author：
Yang Xiaoxia, Xiao Ping, Shi Xiaofeng
IF：
2.8000
Publish_to：
MOLECULAR BIOLOGY REPORTS
PMID：
37812357
Chemerin promotes MAPK/ERK activation to induce inflammatory factor production in rat synoviocytes
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Author：
Chen Wang, Songrui Zhang, Longao Huang, Jianyi Liu, Quan Zhou, Gang Du, Shan Lao
IF：
2.7510
Publish_to：
Experimental and Therapeutic Medicine
PMID：
36277163
Role of dexmedetomidine in postoperative cognitive dysfunction and sleep improvement in aged rats by regulating the PI3K/Akt signaling pathway and its mechanism
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Author：
Ying Kong, Xiaopeng Wang, Jun Pang, Haiyan Huo, Xiaofang Wang
IF：
2.7000
Publish_to：
BRAIN RESEARCH
PMID：
39892804
Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating H2S/CSE and NO/nNOS Systems
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Author：
Ying Liu, Wenhua Wei, Shiwei Liang, Haicheng Fang, Jie Cao
IF：
2.6500
Publish_to：
Evidence-based Complementary and Alternative Medicine
PMID：
36091585
Tilianin extracted from Xiangqinglan () inhibits apoptosis induced by mitochondrial pathway and endoplasmic reticulum stress in H9c2 cells after oxygen-glucose deprivation/reoxygenation.
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Author：
Wen JIANG, Wei ZHANG, Yuxiang ZHANG, Hao YANG, Xiaomei PAN, Qiang CHEN, Junhui CHEN
IF：
2.6000
Publish_to：
Journal of Traditional Chinese Medicine
PMID：
36639994
Remifentanil reduces multiple organ and energy metabolism disturbances in a rat sepsis model.
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Author：
Yang MX, Wu ZZ, Liao XY, Zhang BL, Chen X, Wu Y, Lin JD
IF：
2.5890
Publish_to：
JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
PMID：
35639040
Milk Fat Globule-EGF Factor 8 (MFGE8) Mitigates Cognitive Impairment in Rats with Sepsis-Associated Encephalopathy: An fMRI Study
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Author：
Haojia Li, Hongsen Liao, Lu Sun, Dejian Chen, Yong Fei, Ming Yao, Bing Huang, Xiaoyan Guo, Shaozheng Song, Hongguang Bao
IF：
2.5000
Publish_to：
Journal of Integrative Neuroscience
PMID：
39082291
Toxicopathological, proinflammatory and stress response evaluation of Terminalia catappa extract in male Wistar rats
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Author：
Franklyn Nonso Iheagwam, Chiamaka Obiageli Okeke, Opeyemi Christianah De Campos, Bose Esther Adegboye, Olubanke Olujoke Ogunlana, Shalom Nwodo Chinedu
IF：
2.3000
Publish_to：
Toxicology Reports
PMID：
34712595
Mechanism of action of exercise regulating intestinal microflora to improve spontaneous hypertension in rats
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Author：
Yu Li, Xiaoju Song, Lianjing Dai, Yangyi Wang, Qiong Luo, Lei Lei, Yunfei Pu
IF：
2.2000
Publish_to：
Biomolecules and Biomedicine
PMID：
39484785
Ginsenoside Rg1 relieves rat intervertebral disc degeneration and inhibits IL-1β-induced nucleus pulposus cell apoptosis and inflammation via NF-κB signaling pathway
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Author：
Yu Lei, Hao Ying-Jie, Ren Zhi-Nan, Zhu Guang-Duo, Zhou Wei-Wei, Lian Xu, Wu Xue-Jian
IF：
2.1000
Publish_to：
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
PMID：
38485818
Effect of 3D printing technology-assisted TKA on cartilage tissue in rabbit with knee osteoarthritis.
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Author：
Wang G, Cheng F, Cui J, Chen N, Li Q
IF：
2.0000
Publish_to：
HISTOLOGY AND HISTOPATHOLOGY
PMID：
38639204
Esculetin attenuates cerebral ischemia-reperfusion injury and protects neurons through Nrf2 activation in rats
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Author：
Zhang Zhe, Zhang Jiayun, Shi Rui, Xu Tiantian, Wang Shiduo, Tian Junbiao
IF：
1.9000
Publish_to：
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
PMID：
39504067
MiR-210-3p Enhances Cardiomyocyte Apoptosis and Mitochondrial Dysfunction by Targeting the NDUFA4 Gene in Sepsis-Induced Myocardial Dysfunction
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Author：
Dandan Chen, Yu Hou, Xingjun Cai
IF：
1.8620
Publish_to：
International Heart Journal
PMID：
33994501
Platelet-rich fibrin attenuates inflammation and fibrosis in vulvar lichen sclerosus via the TGF-β/SMAD3 pathway
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Author：
Sun Shu-Hong, Fan Hong-He, Wang Xiao-Wei, Bing Bo-Dong, Hu Yuan-Jing
IF：
1.8000
Publish_to：
ARCHIVES OF DERMATOLOGICAL RESEARCH
PMID：
39918612
Resveratrol inhibits multiple organ injury in preeclampsia rat model
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Author：
Xiao Lang, Wei Liu, Qinfang Chen, Xingyu Yang, Dan Chen, Weiwei Cheng
IF：
1.7000
Publish_to：
ACTA BIOCHIMICA POLONICA
PMID：
36735622
Tempol attenuates chronic intermittent hypoxia-induced lung injury through the miR-145-5p/Nrf2 signaling pathway
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Author：
Li Ai, Yongxia Li, Zhijuan Liu, Ran Li, Xiaona Wang, Bing Hai
IF：
1.6000
Publish_to：
CELLULAR AND MOLECULAR BIOLOGY
PMID：
38158661
20(S)-protopanaxatriol inhibited D-galactose-induced brain aging in mice via promoting mitochondrial autophagy flow
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Author：
Qiaosu Xiao, Ting Li, Wenyang He
IF：
0.6000
Publish_to：
TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH
PMID：
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