中文
英语
大鼠白细胞介素1β检测试剂盒
英文名称:Rat IL-1β ELISA Kit
应用: Sandwich ELISA,ELISA
反应性:Rat
样品类型:血清/血浆/细胞培养上清
灵敏度:30pg/mL
储存条件:2-8℃，6 months
产品简介
| 中文名称 | 大鼠白细胞介素1β检测试剂盒 |
| 英文名称 | Rat IL-1β ELISA Kit |
| 别名 | catabolin; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta |
| 储存条件 | 2-8℃，6 months |
| 检测目标 | IL-1β |
| 样品类型 | 血清/血浆/细胞培养上清 |
| 反应性 | Rat |
| 反应类型 | Sandwich-ELISA |
| 定量/定性 | 定量检测 |
| 灵敏度 | 30pg/mL |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| Swiss Prot | Q63264 |
| Gene ID | 24494 |
| 试剂盒类型 | 传统ELISA试剂盒 |
| 检测原理 | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠 IL-1β单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的大鼠 IL-1β会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗大鼠 IL-1β抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠 IL-1β发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的大鼠 IL-1β，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的大鼠 IL-1β浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠 IL-1β的浓度。 |
| 规格 | 48T 96T |
| 应用 | Sandwich ELISA ELISA |
| 疾病系列 | 肥胖研究相关因子 骨质疏松症研究相关因子 冠心病研究相关因子 慢阻肺COPD研究相关因子 骨关节炎相关因子 神经系统疾病相关因子 糖尿病研究相关因子 系统性红斑狼疮相关因子 哮喘相关因子 心血管疾病相关因子 |
| 研究领域 | 心血管 新陈代谢 肿瘤&癌症 免疫学 |
| 单位 | 盒 |
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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FOOD SCIENCE AND BIOTECHNOLOGY
PMID：
37215259
Quercetin Alleviates Neuropathic Pain in the Rat CCI Model by Mediating AMPK/MAPK Pathway
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Author：
Guangyao Ye, Chunyan Lin, Yu Zhang, Zihan Ma, Yuebo Chen, Lingsi Kong, Liyong Yuan, Tao Ma
IF：
2.8320
Publish_to：
Journal of Pain Research
PMID：
34040433
Adsorption Resin/Polyethersulfone Membrane Used for Plasma Separation and Middle Molecular Toxins Adsorption
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Author：
Zuo Ting, Gong Bingzheng, Gao Yang, Yuan Liangjie
IF：
2.8000
Publish_to：
MOLECULAR BIOLOGY REPORTS
PMID：
37344642
Chemerin promotes MAPK/ERK activation to induce inflammatory factor production in rat synoviocytes
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Author：
Chen Wang, Songrui Zhang, Longao Huang, Jianyi Liu, Quan Zhou, Gang Du, Shan Lao
IF：
2.7510
Publish_to：
Experimental and Therapeutic Medicine
PMID：
36277163
Fecal Microbiota Transplantation Alleviated Paclitaxel-Induced Peripheral Neuropathy by Interfering with Astrocytes and TLR4/p38MAPK Pathway in Rats
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Author：
Haibin Shi, Minmin Chen, Caihong Zheng, Bian Yinglin, Bin Zhu
IF：
2.7000
Publish_to：
Journal of Pain Research
PMID：
37483406
Naringin Prevents Cognitive Dysfunction in Aging Rats by Inhibiting Toll-Like Receptor 4 (TLR4)/NF-κB Pathway and Endoplasmic Reticulum Stress
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Author：
Xiao-jie Dai, Yi Jia, Rui Cao, Mei-ning Zhou
IF：
2.6500
Publish_to：
Evidence-based Complementary and Alternative Medicine
PMID：
36865741
NF-κB affected the serum levels of TNF-α and IL-1β via activation of the MAPK/NF-κB signaling pathway in rat model of acute pulmonary microthromboembolism
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Author：
Yanfen Zhong, Binbin Liang, Xiaofeng Zhang, Jingtao Li, Decai Zeng, Tongtong Huang, Ji Wu
IF：
2.6000
Publish_to：
Pulmonary Circulation
PMID：
38584678
Milk Fat Globule-EGF Factor 8 (MFGE8) Mitigates Cognitive Impairment in Rats with Sepsis-Associated Encephalopathy: An fMRI Study
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Author：
Haojia Li, Hongsen Liao, Lu Sun, Dejian Chen, Yong Fei, Ming Yao, Bing Huang, Xiaoyan Guo, Shaozheng Song, Hongguang Bao
IF：
2.5000
Publish_to：
Journal of Integrative Neuroscience
PMID：
39082291
Rapamycin inhibits LOC102553434-mediated pyroptosis to improve lung injury induced by limb ischemia–reperfusion
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Author：
Huang Dan, Zhang Lie-liang, Zhou Bin, Luo Zhen-Zhong, zhang Jing, Tang Bin, Yu Shu-Chun
IF：
2.4060
Publish_to：
3 Biotech
PMID：
34221806
The extract of concentrated growth factor enhances osteogenic activity of osteoblast through PI3K/AKT pathway and promotes bone regeneration in vivo
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Author：
Dong Kai, Zhou Wen-Juan, Liu Zhong-Hao, Hao Peng-Jie
IF：
2.3840
Publish_to：
International Journal of Implant Dentistry
PMID：
34345951
Protective effect of polyphenols from Potentilla anserina L. against in rat myocardial ischemia-reperfusion on injury by Wnt/β-catenin pathway
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Author：
Jing Li, Meng-Yu Liu, Jian-Guo Li, He Zhang, Chun-Jie Zhang, Wei-Yi Ao, Ming Zhang, Xia-Guang Duan, Zhi-Ying Zhao
IF：
2.3000
Publish_to：
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES
PMID：
40110986
Ginsenoside Rg1 relieves rat intervertebral disc degeneration and inhibits IL-1β-induced nucleus pulposus cell apoptosis and inflammation via NF-κB signaling pathway
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Author：
Yu Lei, Hao Ying-Jie, Ren Zhi-Nan, Zhu Guang-Duo, Zhou Wei-Wei, Lian Xu, Wu Xue-Jian
IF：
2.1000
Publish_to：
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
PMID：
38485818
Effect of 3D printing technology-assisted TKA on cartilage tissue in rabbit with knee osteoarthritis.
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Author：
Wang G, Cheng F, Cui J, Chen N, Li Q
IF：
2.0000
Publish_to：
HISTOLOGY AND HISTOPATHOLOGY
PMID：
38639204
Esculetin attenuates cerebral ischemia-reperfusion injury and protects neurons through Nrf2 activation in rats
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Author：
Zhang Zhe, Zhang Jiayun, Shi Rui, Xu Tiantian, Wang Shiduo, Tian Junbiao
IF：
1.9000
Publish_to：
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
PMID：
39504067
MiR-3571 modulates traumatic brain injury by regulating the PI3K/AKT signaling pathway via Fbxo31
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Author：
Zhang Yang, He Zongying, Hu Qiongfang, Liu Huali, Wen Rongai, Ru Na, Yu Jinghua, Lv Shaokun, Tao Rui
IF：
1.8000
Publish_to：
CELL BIOCHEMISTRY AND BIOPHYSICS
PMID：
39080190
The protective effects of miR-128-3p on sevoflurane-induced progressive neurotoxicity in rats by targeting NOVA1
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Author：
Dedong Li, Jian Sun, Mingdong Yu, Ying Wang, Yuechun Lu, Bo Li
IF：
1.7920
Publish_to：
JOURNAL OF TOXICOLOGICAL SCIENCES
PMID：
35110470
Tempol attenuates chronic intermittent hypoxia-induced lung injury through the miR-145-5p/Nrf2 signaling pathway
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Author：
Li Ai, Yongxia Li, Zhijuan Liu, Ran Li, Xiaona Wang, Bing Hai
IF：
1.6000
Publish_to：
CELLULAR AND MOLECULAR BIOLOGY
PMID：
38158661
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