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小鼠白细胞介素10检测试剂盒
英文名称:Mouse IL-10 ELISA Kit
应用: Sandwich ELISA,ELISA
反应性:Mouse
样品类型:血清/血浆/细胞培养上清
灵敏度:15.63 pg/ml
储存条件:2-8℃，6 months
产品简介
| 中文名称 | 小鼠白细胞介素10检测试剂盒 |
| 英文名称 | Mouse IL-10 ELISA Kit |
| 别名 | CSIF; GVHDS; IL10A; TGIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL-10MGC126451 |
| 储存条件 | 2-8℃，6 months |
| 检测目标 | IL-10 |
| 样品类型 | 血清/血浆/细胞培养上清 |
| 反应性 | Mouse |
| 反应类型 | Sandwich-ELISA |
| 定量/定性 | 定量检测 |
| 检测范围 | 31.25-2000 pg/ml |
| 灵敏度 | 15.63 pg/ml |
| 特异性 | 不与小鼠IL-2,3,4,5,6,7,9,10Rα反应，人IL-10等反应。 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 反应时间 | 3h |
| Swiss Prot | P18893 |
| Gene ID | 16153 |
| 试剂盒类型 | 传统ELISA试剂盒 |
| 检测原理 | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗小鼠IL-10单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的小鼠IL-10会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗小鼠IL-10抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的小鼠IL-10发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的小鼠IL-10，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的小鼠IL-10浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中小鼠IL-10的浓度。 |
| 规格 | 48T 96T |
| 应用 | Sandwich ELISA ELISA |
| 疾病系列 | 肥胖研究相关因子 骨质疏松症研究相关因子 冠心病研究相关因子 慢阻肺COPD研究相关因子 骨关节炎相关因子 神经系统疾病相关因子 糖尿病研究相关因子 系统性红斑狼疮相关因子 哮喘相关因子 心血管疾病相关因子 |
| 研究领域 | 新陈代谢 肿瘤&癌症 免疫学 |
| 单位 | 盒 |
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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Frontiers in Nutrition
PMID：
40206949
Structural characterization of Aspalatus linearis polysaccharide and its improving effect on acute alcoholic liver injury
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Author：
Yan Zhang, Jia Liu, Shan Xu, Manhong Luo, Shuhan Yang, Siyu Yu
IF：
3.9000
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JOURNAL OF FOOD SCIENCE
PMID：
38809252
Stimulation of lipopolysaccharide from Pseudomonas aeruginosa following H9N2 IAV infection exacerbates inflammatory responses of alveolar macrophages and decreases virus replication
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Kang Shun, Sun Ying-Li, Li Zhi-juan, Li Jian-liang, Xiao Feng, Dong Lu-jiao, Yuan peng, Shijin Jiang, Xie Zhi-jing
IF：
3.8000
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MICROBIAL PATHOGENESIS
PMID：
37481007
Triptolide attenuates LPS-induced activation of RAW 264.7 macrophages by inducing M1-to-M2 repolarization via the mTOR/STAT3 signaling
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Author：
Huanhuan Zhu, Shaopeng Tong, Congrong Yan, Acheng Zhou, Minying Wang, Chunwei Li
IF：
3.7120
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IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
PMID：
35793125
Ferrostatin-1 Polarizes Microglial Cells Toward M2 Phenotype to Alleviate Inflammation After Intracerebral Hemorrhage
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Author：
Huang Lijuan, Zhang Yanjiao, Zhao Liang, Chen Qingyou, Li Li
IF：
3.5320
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Neurocritical Care
PMID：
35099711
Increased Pulmonary Pneumococcal Clearance after Resolution of H9N2 Avian Influenza Virus Infection in Mice
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Author：
Jingyun Li, Hongyan Wang, Pengjing Lian, Yu Bai, Zihui Zhang, Lihong Zhao, Tong Xu, Jian Qiao
IF：
3.4410
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INFECTION AND IMMUNITY
PMID：
33722928
Characterization of genetic humanized mice with transgenic HLA DP401 or DRA but deficient in endogenous murine MHC class II genes upon Staphylococcus aureus pneumonia
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Author：
Feng Li, Bowen Niu, Lingling Liu, Mengmin Zhu, Hua Yang, Boyin Qin, Xiuhua Peng, Lixiang Chen, Chunhua Xu, Xiaohui Zhou
IF：
3.4000
Publish_to：
Animal Models and Experimental Medicine
PMID：
37246733
Physicochemical, morpho-structural, and biological characterization of polysaccharides from three Polygonatum spp
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Author：
Jin-Bo Bai, Ji-Chun Ge, Wang-Juan Zhang, Wang Liu, Jian-Ping Luo, Feng-Qing Xu, De-Ling Wu, Song-Zi Xie
IF：
3.3610
Publish_to：
RSC Advances
PMID：
35498116
Regulation of colon injury and improvement of exercise performance in exhausted running mice by Lactobacillus pentosus CQZC02
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Author：
Limin Cai, Beibei Wang
IF：
3.2000
Publish_to：
Frontiers in Physiology
PMID：
39371599
miR-130a-3p enhances autophagy through the YY1/PI3K/AKT/mTOR signaling pathway to regulate macrophage polarization and alleviate diabetic retinopathy
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Author：
Xiaoting Xi, Xuewei Wang, Jia Ma, Qianbo Chen, Yuxin Zhang, Yaxian Song, Yan Li
IF：
3.1000
Publish_to：
Journal of Diabetes Investigation
PMID：
39668747
Cryoablation plus chemotherapy regimen enhance anti-tumor immune response in a mouse model of Lewis lung cancer
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Author：
Hua Duan, Li Yang, Xueni Fang, Shaohua Yan, Yang Cao, Bingli Qiao, Tian Zhou, Kaiwen Hu
IF：
3.0000
Publish_to：
INTERNATIONAL JOURNAL OF HYPERTHERMIA
PMID：
39727085
More Ultrasound-Guided Percutaneous Microwave Ablation Leads to Higher Immune-Related Gene Expression and Boosts PD-1 Monoclonal Antibodies for Liver Cancer
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Author：
Ting Luo, Zhen Wang, Xiaoling Yu, Zhiyu Han, Zhigang Cheng, Fangyi Liu, Jie Yu, Ping Liang
IF：
2.9000
Publish_to：
ULTRASOUND IN MEDICINE AND BIOLOGY
PMID：
37867090
Synthesis and bioactivity evaluation of mirror isomer salidroside derivatives as potent antioxidant and anti-inflammatory agents
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Author：
Zipeng Li, Ao Sun, Xin Wang, Munila Abulimiti, Zhongtang Li, Zhongjun Li
IF：
2.4000
Publish_to：
CARBOHYDRATE RESEARCH
PMID：
38865798
Hypoxia activates FGF-23-ERK / MAPK signaling pathway in ischemia-reperfusion induced acute kidney injury
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Author：
Liu Weihua, Lin Miao, Dai Yiping, Hong Fuyuan
IF：
2.3000
Publish_to：
KIDNEY & BLOOD PRESSURE RESEARCH
PMID：
39413750
Boosting PRRSV-Specific Cellular Immunity: The Immunological Profiling of an Fc-Fused Multi-CTL Epitope Vaccine in Mice
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Author：
Xinnuo Lei, Jinzhao Ban, Zhi Wu, Shinuo Cao, Mo Zhou, Li Zhang, Rui Zhu, Huipeng Lu, Shanyuan Zhu
IF：
2.0000
Publish_to：
Veterinary Sciences
PMID：
38922021
miR-874-3p Alleviates Macrophage-Mediated Inflammatory Injury in Intracerebral Hemorrhage by Targeting HIPK2
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Author：
Shu Quan, Lai Ruihui
IF：
1.8000
Publish_to：
CELL BIOCHEMISTRY AND BIOPHYSICS
PMID：
39298065
Regulation of Fuzheng Huayu capsule on inhibiting the fibrosis-associated hepatocellular carcinogenesis
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Author：
Wen-Qi Zhang, Jia-Xin Sun, Shu-Ting Lan, Xiao-Mei Sun, Yi-Jing Guo, Bi-Chao Wen, Jie Chen, Gang Liu
IF：
1.7000
Publish_to：
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
PMID：
38780602
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