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人肿瘤坏死因子α检测试剂盒
英文名称:Human TNF-α ELISA Kit
应用: Sandwich ELISA,ELISA
反应性:Human
样品类型:血清/血浆/细胞培养上清
灵敏度:8pg/ml
储存条件:2-8℃，6 months
产品简介
| 中文名称 | 人肿瘤坏死因子α检测试剂盒 |
| 英文名称 | Human TNF-α ELISA Kit |
| 别名 | APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha |
| 储存条件 | 2-8℃，6 months |
| 检测目标 | TNF-α |
| 样品类型 | 血清/血浆/细胞培养上清 |
| 反应性 | Human |
| 反应类型 | Sandwich-ELISA |
| 定量/定性 | 定量检测 |
| 检测范围 | 15.62-1000pg/ml |
| 灵敏度 | 8pg/ml |
| 特异性 | 不与人TNF-β、人GM-CSF、人sTNFRI、人sTNFRII、小鼠TNF-α、小鼠sTNFRI等反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| Swiss Prot | P01375 |
| Gene ID | 7124 |
| 试剂盒类型 | 传统ELISA试剂盒 |
| 检测原理 | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人TNF-α单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人TNF-α会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗人TNF-α抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人TNF-α发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人TNF-α，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人TNF-α浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人TNF-α的浓度。 |
| 规格 | 48T 96T |
| 应用 | Sandwich ELISA ELISA |
| 疾病系列 | 肥胖研究相关因子 骨质疏松症研究相关因子 冠心病研究相关因子 慢阻肺COPD研究相关因子 骨关节炎相关因子 神经系统疾病相关因子 糖尿病研究相关因子 系统性红斑狼疮相关因子 哮喘相关因子 心血管疾病相关因子 |
| 研究领域 | 心血管 新陈代谢 微生物 细胞生物学 信号传导 肿瘤&癌症 免疫学 |
| 单位 | 盒 |
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
实验图
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Erythropoietin promotes energy metabolism to improve LPS-induced injury in HK-2 cells via SIRT1/PGC1-α pathway
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Author：
Li Kan, Gao Li, Zhou Sen, Ma Yan-Rong, Xiao Xiao, Jiang Qian, Kang Zhi-Hong, Liu Ming-Long, Liu Tian-Xi
IF：
3.8420
Publish_to：
MOLECULAR AND CELLULAR BIOCHEMISTRY
PMID：
36001204
Dabie bandavirus infection induces macrophagic pyroptosis and this process is attenuated by platelets
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Author：
Sicong Yu, Qinyi Zhang, Lingxuan Su, Ji He, Wen Shi, Hao Yan, Haiyan Mao, Yi Sun, Dongqing Cheng, Xuan Wang, Yanjun Zhang, Lei Fang
IF：
3.8000
Publish_to：
PLoS Neglected Tropical Diseases
PMID：
37486928
Tanshinone IIA-Loaded Micelles Functionalized with Rosmarinic Acid: A Novel Synergistic Anti-Inflammatory Strategy for Treatment of Atherosclerosis
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Author：
Meixuan Liu, Sha Liu, Xiaosu Zhu, Yiying Sun, Linyu Su, Hairong Yu, Deshuai Liu, Ying Li, Yuan Du, Rongxia Liu, Kaoxiang Sun
IF：
3.7840
Publish_to：
JOURNAL OF PHARMACEUTICAL SCIENCES
PMID：
35580692
Quantitative proteomics analysis of COVID-19 patients: Fetuin-A and tetranectin as potential modulators of innate immune responses
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Author：
Bandar Alghanem, Fatmah A Mansour, Hayat Shaibah, Khawlah Almuhalhil, Feras Almourfi, Hassan S Alamri, Hala Alajmi, Mamoon Rashid, Fayhan Alroqi, Maroua Jalouli, Abdel Halim Harrath, Mohammad Boudjellal, Tlili Barhoumi
IF：
3.7760
Publish_to：
Heliyon
PMID：
37064481
Deuterohemin-Ala-His-Thr-Val-Glu-Lys (DhHP-6) Mimicking Enzyme as Synergistic Antioxidant and Anti-Inflammatory Material for Periodontitis Therapy
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Author：
Jiaqing Yan, Min Liu, Yan Zhang, Ying Zhu, Qiuyan Chen, Yimeng Yang, Min Hu, Huimei Yu
IF：
3.7430
Publish_to：
Biomimetics
PMID：
36546940
TRIM56-mediated production of type I interferon inhibits intracellular replication of Rickettsia rickettsii
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Author：
Ruxi Cheng, Chunyu Zhou, Mingliang Zhao, Shan Zhang, Weiqiang Wan, Yonghui Yu, Bohai Wen, Jun Jiao, Xiaolu Xiong, Qin Xu, Xuan OuYang
IF：
3.7000
Publish_to：
Microbiology Spectrum
PMID：
38358243
Knockdown of circ_0007290 alleviates oxygen-glucose deprivation-induced neuronal injury by regulating miR-496/PDCD4 axis
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Author：
Wang Fengjuan, Liu Jie, Wang Dan, Yao Yu, Jiao Xuhua
IF：
3.6550
Publish_to：
METABOLIC BRAIN DISEASE
PMID：
35032277
Circ_0000566 contributes oxygen–glucose deprivation and reoxygenation (OGD/R)-induced human brain microvascular endothelial cell injury via regulating miR-18a-5p/ACVR2B axis
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Author：
Liu Dan, Xiao Haitao, Liu Jinxing, Zhang Yixin, Li Jialiang, Zhang Tingyu, Chen Honglin
IF：
3.6000
Publish_to：
METABOLIC BRAIN DISEASE
PMID：
36781583
Exosomes miR-92a-3p from human exfoliated deciduous teeth inhibits periodontitis progression via the KLF4/PI3K/AKT pathway
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Author：
Tianliang Yu, Na Mi, Yingtao Song, Weili Xie
IF：
3.5000
Publish_to：
JOURNAL OF PERIODONTAL RESEARCH
PMID：
38616305
Quercetin affects apoptosis and autophagy in pediatric acute myeloid leukaemia cells by inhibiting PI3K/AKT signaling pathway activation through regulation of miR-224-3p/PTEN axis
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Author：
Jing Sun, Min Sha, Jing Zhou, Yun Huang
IF：
3.4000
Publish_to：
BMC CANCER
PMID：
39984900
Downregulation of LINC00221 promotes angiopoiesis in HUVEC and inhibits recruitment of macrophages by augmenting miR-542-3p in trophoblast cells
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Author：
Zhao Guangzhi, Wang Yuan, Wang Yingying, Cui Xueyin, Liu Ling, Zhi Yunxiao, Han Xiao, Zhao Lanlan, Chen Juan, Shi Zhuolin, Cui Shihong
IF：
3.3570
Publish_to：
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
PMID：
36069974
TLR9 regulates the autophagy每lysosome pathway to promote dendritic cell maturation and activation by activating the TRAF6-cGAS-STING pathway
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Author：
Ying Liu, Fang-Zhi Wei, Yu-Wei Zhan, Ru Wang, Bi-Yao Mo, Shu-Dian Lin
IF：
3.3000
Publish_to：
KAOHSIUNG JOURNAL OF MEDICAL SCIENCES
PMID：
37850718
CircLPAR1/miR-212-3p/ZNF217 feedback loop promotes amyloid β-induced neuronal injury in Alzheimer’s Disease
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Author：
Lifang Wu, Qiang Du, Congcong Wu
IF：
3.2520
Publish_to：
BRAIN RESEARCH
PMID：
34403662
miR-148a-3p mitigation of coronary artery disease through PCSK9/NF-κB inhibition of vascular endothelial cell injury
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Author：
Jiong Tang, Menghuai Ma, Fan Liu, Xiaomei Yin, Haotian Shi, Qing Li, Kai Yang, Mengyue Yu
IF：
3.2000
Publish_to：
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
PMID：
39400940
Circular RNA Circ-BANP Regulates Oxidized Low-density Lipoprotein-induced Endothelial Cell Injury Through Targeting the miR-370/Thioredoxin-interacting Protein Axis
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Author：
Chen Guang, Li Yongjun, Zhang Ailing, Gao Lijun
IF：
3.1050
Publish_to：
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
PMID：
33298736
miR-130a-3p enhances autophagy through the YY1/PI3K/AKT/mTOR signaling pathway to regulate macrophage polarization and alleviate diabetic retinopathy
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Author：
Xiaoting Xi, Xuewei Wang, Jia Ma, Qianbo Chen, Yuxin Zhang, Yaxian Song, Yan Li
IF：
3.1000
Publish_to：
Journal of Diabetes Investigation
PMID：
39668747
Modified TPP-MoS2 QD Blend as a Bio-Functional Model for Normalizing Microglial Dysfunction in Alzheimer＊s Disease
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Author：
Ohoud A. Alomari, Safaa Qusti, Maha Balgoon, Fadwa Aljoud, Khalid A. Alamry, Mahmoud A. Hussein
IF：
3.0000
Publish_to：
Neurology International
PMID：
37606394
Fluorofenidone attenuates paraquat‑induced pulmonary fibrosis by regulating the PI3K/Akt/mTOR signaling pathway and autophagy
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Author：
Feiya Jiang, Sha Li, Yu Jiang, Zhuo Chen, Tongtong Wang, Wen Liu
IF：
2.9520
Publish_to：
Molecular Medicine Reports
PMID：
33786626
Circ-BNIP3L knockdown alleviates LPS-induced renal tubular epithelial cell injury during sepsis-associated acute kidney injury by miR-370-3p/MYD88 axis
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Author：
Zhou Yanyan, Qing Meiying, Xu Min
IF：
2.9450
Publish_to：
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
PMID：
34731384
Circ-RBMS1 Knockdown Alleviates CSE-Induced Apoptosis, Inflammation and Oxidative Stress via Up-Regulating FBXO11 Through miR-197-3p in 16HBE Cells
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Author：
Di Qiao, Chi Hu, Qiuyan Li, Jun Fan
IF：
2.8930
Publish_to：
International Journal of Chronic Obstructive Pulmonary Disease
PMID：
34295155
Potential mechanism of Taohong Siwu Decoction in preventing and treating postoperative delirium in intertrochanteric fracture patients based on retrospective analysis and network pharmacology
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Author：
Ding Zhihong, Yu Zhiyong, Sun Zhibo, Liu Xinghui, Chen Rong
IF：
2.8000
Publish_to：
Journal of Orthopaedic Surgery and Research
PMID：
38902693
Triptolide improves Alzheimer’s disease by regulating the NF signaling pathway through the lncRNA NEAT1/microRNA 361 3p/TRAF2 axis
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Author：
Li Zhou, Xuming Huang, Haiyan Li, Jihui Wang, Zhengqi Lu
IF：
2.7000
Publish_to：
Experimental and Therapeutic Medicine
PMID：
37614428
Additional Acupuncture Confers a Favorable Long-Term Prognosis for Elderly Hypertensive Patients with Carotid Atherosclerosis after Atorvastatin Treatment
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Author：
Jiefang Li, Dan Wu, Jun Liu, Xi Li
IF：
2.6500
Publish_to：
Evidence-based Complementary and Alternative Medicine
PMID：
35615686
Inhibitory Effect of Paeonol on Apoptosis, Oxidative Stress, and Inflammatory Response in Human Umbilical Vein Endothelial Cells Induced by High Glucose and Palmitic Acid Induced Through Regulating SIRT1/FOXO3a/NF-κB Pathway
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Author：
Hanqing Tang, Keming Li, Shitian Zhang, Huangqi Lan, Lingling Liang, Cenhan Huang, Tianzi Li
IF：
2.6070
Publish_to：
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
PMID：
33750217
DNMT1 inhibition improves the activity of memory-like natural killer cells by enhancing the level of autophagy
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Author：
Li Yixun, Guo Chong, Zhang Fujia, Cheng Shenju, Li Yanhong, Luo Shan, Zeng Yun, Zhao Yaling, Wu Kun
IF：
2.6000
Publish_to：
MOLECULAR BIOLOGY REPORTS
PMID：
39704855
Silencing fatty acid-binding protein 4 improved sepsis-induced myocardial dysfunction through anti-apoptotic and antioxidant effects by mammalian target of rapamycin signaling pathway
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Author：
Zhilei Qiu, Kexing Zhou, Qinyao Qi, Wei Chen
IF：
2.5000
Publish_to：
CytoJournal
PMID：
39958886
IRF2BP2 prevents ox‑LDL‑induced inflammation and EMT in endothelial cells via regulation of KLF2
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Author：
Yongri Jiang, Qiuling Shen
IF：
2.4470
Publish_to：
Experimental and Therapeutic Medicine
PMID：
33767776
Resveratrol Inhibits circ_0074371-related Pathway to Alleviate Sepsis-induced Acute Kidney Injury
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Author：
Zhu Dongju, Wu Xiang
IF：
2.4000
Publish_to：
BIOCHEMICAL GENETICS
PMID：
37730967
Implications of the SNHG10/miR-665/RASSF5/NF-κB pathway in dihydromyricetin-mediated ischemic stroke protection
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Author：
Qi Zeng, Yan Xiao, Xueliang Zeng, Hai Xiao
IF：
2.3000
Publish_to：
PeerJ
PMID：
39726744
High level of C3 is associated with Th2 immune response and liver fibrosis in patients with schistosomiasis
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Author：
Xianmo Wang, Quan Gong, Hao Nie, Jiancheng Tu, Wen Fan, Xiaoping Tan
IF：
2.2000
Publish_to：
PARASITE IMMUNOLOGY
PMID：
38465509
Interference of periostin attenuates pathological changes,proinflammatory markers and renal fibrosis in diabetic kidney injury
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Author：
Duan Xiaoting, Chen Cheng, Liu Xiaoli, Wang Taoxia, Feng Shuning, Li Jianwei, Li Guiying
IF：
2.1640
Publish_to：
Genes & Genomics
PMID：
37248423
SOS1-IT1 silencing alleviates MPP+-induced neuronal cell injury through regulating the miR-124-3p/PTEN/AKT/mTOR pathway
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Author：
Jianhu Fan, Dahua Wu, Yuxing Guo, Zhongbao Yang
IF：
2.1160
Publish_to：
JOURNAL OF CLINICAL NEUROSCIENCE
PMID：
35279586
Circ_0059662 exerts a positive role in oxygen每glucose deprivation/reoxygenation-induced SK-N-SH cell injury
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Author：
An Yang, Xu Dan, Yuan Lei, Wen Ying
IF：
2.0000
Publish_to：
EXPERIMENTAL BRAIN RESEARCH
PMID：
37815551
Astragaloside IV relieves IL-1汕-induced human nucleus pulposus cells degeneration through modulating PI3K/Akt signaling pathway
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Author：
Lu Zhang, Junsheng Gao, Zhentao Li, Jun Liu, Chong Zhang, Jie Liu, Hui Dong, Wei Mei
IF：
1.6000
Publish_to：
MEDICINE
PMID：
37603510
Atorvastatin inhibits ischemia‒reperfusion-associated renal tubular cell ferroptosis by blocking the PGE2/EP4 signaling pathway
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Author：
Yang Jing, Zhou Rongrong, Zhou Mengjiao, Li Xinghuan
IF：
1.5000
Publish_to：
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
PMID：
39920560
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