人白细胞介素1β检测试剂盒
英文名称:Human IL-1β ELISA Kit
应用: Sandwich ELISA,ELISA
反应性:Human
样品类型:血清/血浆/细胞培养上清
灵敏度:2pg/mL
储存条件:2-8℃,6 months


产品简介
中文名称 | 人白细胞介素1β检测试剂盒 |
英文名称 | Human IL-1β ELISA Kit |
别名 | catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta |
储存条件 | 2-8℃,6 months |
检测目标 | IL-1β |
样品类型 | 血清/血浆/细胞培养上清 |
反应性 | Human |
反应类型 | Sandwich-ELISA |
定量/定性 | 定量检测 |
灵敏度 | 2pg/mL |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
Swiss Prot | P01584 |
Gene ID | 3553 |
试剂盒类型 | 传统ELISA试剂盒 |
检测原理 | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人IL-1β单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品和预稀释的样本,标准品和样本中的人IL-1β会与酶标板上的包被抗体充分结合;洗板后加入生物素化抗人IL-1β抗体,该抗体会与板子上包被抗体捕获的标准品和样本中的人IL-1β发生特异性结合;洗板后加入辣根过氧化物酶(HRP)标记的链霉亲和素,生物素与链霉亲和素会发生高强度的非共价结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的人IL-1β,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450 nm(OD=450 nm)处测定反应孔样品吸光度(OD),样本中的人IL-1β浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中人IL-1β的浓度。 |
规格 | 48T 96T |
应用 | Sandwich ELISA ELISA |
疾病系列 | 肥胖研究相关因子 骨质疏松症研究相关因子 冠心病研究相关因子 慢阻肺COPD研究相关因子 骨关节炎相关因子 神经系统疾病相关因子 糖尿病研究相关因子 系统性红斑狼疮相关因子 哮喘相关因子 心血管疾病相关因子 |
研究领域 | 心血管 新陈代谢 肿瘤&癌症 免疫学 |
单位 | 盒 |
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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Li Kan, Gao Li, Zhou Sen, Ma Yan-Rong, Xiao Xiao, Jiang Qian, Kang Zhi-Hong, Liu Ming-Long, Liu Tian-Xi
IF:
3.8420
Publish_to:
MOLECULAR AND CELLULAR BIOCHEMISTRY
PMID:
36001204
Dabie bandavirus infection induces macrophagic pyroptosis and this process is attenuated by platelets
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Author:
Sicong Yu, Qinyi Zhang, Lingxuan Su, Ji He, Wen Shi, Hao Yan, Haiyan Mao, Yi Sun, Dongqing Cheng, Xuan Wang, Yanjun Zhang, Lei Fang
IF:
3.8000
Publish_to:
PLoS Neglected Tropical Diseases
PMID:
37486928
Deuterohemin-Ala-His-Thr-Val-Glu-Lys (DhHP-6) Mimicking Enzyme as Synergistic Antioxidant and Anti-Inflammatory Material for Periodontitis Therapy
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Author:
Jiaqing Yan, Min Liu, Yan Zhang, Ying Zhu, Qiuyan Chen, Yimeng Yang, Min Hu, Huimei Yu
IF:
3.7430
Publish_to:
Biomimetics
PMID:
36546940
Circ_0003972 Promotes the Proliferation and Inflammation of Fibroblast-like Synovial Cells in Rheumatoid Arthritis through Regulation of the miR-654-5p/FZD4 Axis
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Author:
Juan Li, Dan Liu, Kunyi Wu, Qun Chen, Jianhong Lei
IF:
3.6570
Publish_to:
IMMUNOLOGICAL INVESTIGATIONS
PMID:
34325604
Knockdown of circ_0007290 alleviates oxygen-glucose deprivation-induced neuronal injury by regulating miR-496/PDCD4 axis
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Author:
Wang Fengjuan, Liu Jie, Wang Dan, Yao Yu, Jiao Xuhua
IF:
3.6550
Publish_to:
METABOLIC BRAIN DISEASE
PMID:
35032277
Saikosaponin-D induces the pyroptosis of lung cancer by increasing ROS and activating the NF-κB/NLRP3/caspase-1/GSDMD pathway
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Author:
Mengqing Chen, Chunyan Hu, Lei Yang, Qingxi Guo, Yuling Liang, Wenjun Wang
IF:
3.6000
Publish_to:
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
PMID:
37393521
Penthorum chinense Pursh extract ameliorates hepatic steatosis by suppressing pyroptosis via the NLRP3/Caspase-1/GSDMD pathway
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Author:
Ruixi Luo, Yudie Hu, La Wang, Zunli Ke, Wenjia Wang, Ping Wang, Weiyi Tian
IF:
3.5000
Publish_to:
Food Science & Nutrition
PMID:
39055201
Exosomes miR-92a-3p from human exfoliated deciduous teeth inhibits periodontitis progression via the KLF4/PI3K/AKT pathway
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Author:
Tianliang Yu, Na Mi, Yingtao Song, Weili Xie
IF:
3.4000
Publish_to:
JOURNAL OF PERIODONTAL RESEARCH
PMID:
38616305
Effective Improvement of the Oxidative Stability of Acer truncatum Bunge Seed Oil, a New Woody Oil Food Resource, by Rosemary Extract
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Author:
Run-Feng Miao, Jing Tu
IF:
3.3000
Publish_to:
KAOHSIUNG JOURNAL OF MEDICAL SCIENCES
PMID:
37265187
CircLPAR1/miR-212-3p/ZNF217 feedback loop promotes amyloid β-induced neuronal injury in Alzheimer’s Disease
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Author:
Lifang Wu, Qiang Du, Congcong Wu
IF:
3.2520
Publish_to:
BRAIN RESEARCH
PMID:
34403662
4-Octyl itaconate alleviates renal ischemia reperfusion injury by ameliorating endoplasmic reticulum stress via Nrf2 pathway
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Author:
Xiang-Kun Li, Hong-Juan Yang, Shi-Han Du, Bing Zhang, Ling-Yu Li, Shao-Na Li, Cui-Cui Liu, Yang Ma, Jian-Bo Yu
IF:
3.2000
Publish_to:
EXPERIMENTAL BIOLOGY AND MEDICINE
PMID:
38158612
Circular RNA Circ-BANP Regulates Oxidized Low-density Lipoprotein-induced Endothelial Cell Injury Through Targeting the miR-370/Thioredoxin-interacting Protein Axis
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Author:
Chen Guang, Li Yongjun, Zhang Ailing, Gao Lijun
IF:
3.1050
Publish_to:
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
PMID:
33298736
The molecular mechanism of berberine affecting psoriasis skin inflammation by regulating keratinocyte pyroptosis via the p38 MAPK/NF-κB pathway
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Author:
Chen Wenfang, Ge Lingzhi, Zhang Chao
IF:
3.1000
Publish_to:
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
PMID:
39365309
A Novel MIR503HG/miR-497-5p/CCL19 Axis Regulates High Glucose-Induced Cell Apoptosis, Inflammation, and Fibrosis in Human HK-2 Cells
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Author:
Zhang Danping, Chen Xiaoxiao, Zheng Dan
IF:
3.0940
Publish_to:
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
PMID:
35013925
Modified TPP-MoS2 QD Blend as a Bio-Functional Model for Normalizing Microglial Dysfunction in Alzheimer*s Disease
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Author:
Ohoud A. Alomari, Safaa Qusti, Maha Balgoon, Fadwa Aljoud, Khalid A. Alamry, Mahmoud A. Hussein
IF:
3.0000
Publish_to:
Neurology International
PMID:
37606394
Kurarinone alleviates hemin-induced neuroinflammation and microglia-mediated neurotoxicity by shifting microglial M1/M2 polarization via regulating the IGF1/PI3K/Akt signaling
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Author:
Zeng-Qiang Jia, Cheng Zuo, Wen-Feng Yue
IF:
2.9960
Publish_to:
KAOHSIUNG JOURNAL OF MEDICAL SCIENCES
PMID:
36169245
CircSCAPER knockdown attenuates IL-1β-induced chondrocyte injury by miR-127-5p/TLR4 axis in osteoarthritis
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Author:
Yuchang Zhang, Ping Zhao, Sen Li, Xiangqian Mu, Huaqi Wang
IF:
2.9570
Publish_to:
AUTOIMMUNITY
PMID:
35993243
Fluorofenidone attenuates paraquat‑induced pulmonary fibrosis by regulating the PI3K/Akt/mTOR signaling pathway and autophagy
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Author:
Feiya Jiang, Sha Li, Yu Jiang, Zhuo Chen, Tongtong Wang, Wen Liu
IF:
2.9520
Publish_to:
Molecular Medicine Reports
PMID:
33786626
Circ-BNIP3L knockdown alleviates LPS-induced renal tubular epithelial cell injury during sepsis-associated acute kidney injury by miR-370-3p/MYD88 axis
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Author:
Zhou Yanyan, Qing Meiying, Xu Min
IF:
2.9450
Publish_to:
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
PMID:
34731384
Circ-RBMS1 Knockdown Alleviates CSE-Induced Apoptosis, Inflammation and Oxidative Stress via Up-Regulating FBXO11 Through miR-197-3p in 16HBE Cells
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Author:
Di Qiao, Chi Hu, Qiuyan Li, Jun Fan
IF:
2.8930
Publish_to:
International Journal of Chronic Obstructive Pulmonary Disease
PMID:
34295155
CircSHOC2 Knockdown Alleviates High Glucose-Induced Vascular Endothelial Cell Pyroptosis via Targeting miR-145/FOXO1 Axis In Vitro Condition
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Author:
Meng Xuying, Li Zhenjin, Li Yang
IF:
2.8600
Publish_to:
MOLECULAR BIOTECHNOLOGY
PMID:
35960441
Potential mechanism of Taohong Siwu Decoction in preventing and treating postoperative delirium in intertrochanteric fracture patients based on retrospective analysis and network pharmacology
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Author:
Ding Zhihong, Yu Zhiyong, Sun Zhibo, Liu Xinghui, Chen Rong
IF:
2.8000
Publish_to:
Journal of Orthopaedic Surgery and Research
PMID:
38902693
Triptolide improves Alzheimer‘s disease by regulating the NF signaling pathway through the lncRNA NEAT1/microRNA 361 3p/TRAF2 axis
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Author:
Li Zhou, Xuming Huang, Haiyan Li, Jihui Wang, Zhengqi Lu
IF:
2.7000
Publish_to:
Experimental and Therapeutic Medicine
PMID:
37614428
Inhibitory Effect of Paeonol on Apoptosis, Oxidative Stress, and Inflammatory Response in Human Umbilical Vein Endothelial Cells Induced by High Glucose and Palmitic Acid Induced Through Regulating SIRT1/FOXO3a/NF-κB Pathway
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Author:
Hanqing Tang, Keming Li, Shitian Zhang, Huangqi Lan, Lingling Liang, Cenhan Huang, Tianzi Li
IF:
2.6070
Publish_to:
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
PMID:
33750217
Gentiopicroside ameliorates the lipopolysaccharide-induced inflammatory response and hypertrophy in chondrocytes
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Author:
Li Longfei, Fan Qianqian, Zhao Yixuan, Zhang Qian, Qin Gaofeng, Li Chen, Li Wei
IF:
2.6000
Publish_to:
Journal of Orthopaedic Surgery and Research
PMID:
38528538
IL-1 receptor-associated kinase 1 participates in the modulation of the NLRP3 inflammasome by tumor-associated macrophages in hepatocellular carcinoma
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Author:
Wei Chen, Mingjuan Hu, Tao Wei, Ying Liu, Tian Tan, Chengfang Zhang, Jiaxuan Weng
IF:
2.5870
Publish_to:
Journal of Gastrointestinal Oncology
PMID:
35837195
Newcastle disease virus promotes pyroptosis in medulloblastoma cells by regulating interferon-gamma-mediated guanylate-binding protein 1 expression and activating caspase-4
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Author:
Pengwu Ren, Jiayan Yu, Dongxiang Wang, Lijuan Zeng, Xianqiang Zhang, Xiaohe Liu, Yongfu Cao, Zijian Hu, Xiaoyong Zhao, Kongbin Yang
IF:
2.5000
Publish_to:
CytoJournal
PMID:
39563668
Palmitic acid up regulates Gal‑3 and induces insulin resistance in macrophages by mediating the balance between KLF4 and NF‑κB
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Author:
Jing Li, Yu-Shan Mao, Fen Chen, Dong-Xia Xia, Tin-Qi Zhao
IF:
2.4470
Publish_to:
Experimental and Therapeutic Medicine
PMID:
34373714
Resveratrol Inhibits circ_0074371-related Pathway to Alleviate Sepsis-induced Acute Kidney Injury
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Author:
Zhu Dongju, Wu Xiang
IF:
2.4000
Publish_to:
BIOCHEMICAL GENETICS
PMID:
37730967
Implications of the SNHG10/miR-665/RASSF5/NF-κB pathway in dihydromyricetin-mediated ischemic stroke protection
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Author:
Qi Zeng, Yan Xiao, Xueliang Zeng, Hai Xiao
IF:
2.3000
Publish_to:
PeerJ
PMID:
39726744
Interference of periostin attenuates pathological changes,proinflammatory markers and renal fibrosis in diabetic kidney injury
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Author:
Duan Xiaoting, Chen Cheng, Liu Xiaoli, Wang Taoxia, Feng Shuning, Li Jianwei, Li Guiying
IF:
2.1640
Publish_to:
Genes & Genomics
PMID:
37248423
SOS1-IT1 silencing alleviates MPP+-induced neuronal cell injury through regulating the miR-124-3p/PTEN/AKT/mTOR pathway
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Author:
Jianhu Fan, Dahua Wu, Yuxing Guo, Zhongbao Yang
IF:
2.1160
Publish_to:
JOURNAL OF CLINICAL NEUROSCIENCE
PMID:
35279586
Circ_0059662 exerts a positive role in oxygen每glucose deprivation/reoxygenation-induced SK-N-SH cell injury
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Author:
An Yang, Xu Dan, Yuan Lei, Wen Ying
IF:
2.0000
Publish_to:
EXPERIMENTAL BRAIN RESEARCH
PMID:
37815551
CircTLK1 Downregulation Attenuates High Glucose-Induced Human Mesangial Cell Injury by Blocking the AKT/NF-κB Pathway Through Sponging miR-126-5p/miR-204-5p
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Author:
Qiu Binghua, Qi Xin, Wang Juan
IF:
1.8900
Publish_to:
BIOCHEMICAL GENETICS
PMID:
34731387
308-nm excimer laser combined with piperine promotes melanin synthesis through PTEN/PDK1/GSK3b regulation of keratinocyte paracrine
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Author:
Wu Yifei, Wang Xiaochuan, Liu Jing, Wang Zhiqiong, Li Jinrong, Zhang Wenhe, Xiang Jie, Li Renfu, Du Sha, Ao Chunping
IF:
1.8000
Publish_to:
ARCHIVES OF DERMATOLOGICAL RESEARCH
PMID:
40105984
Tempol attenuates chronic intermittent hypoxia-induced lung injury through the miR-145-5p/Nrf2 signaling pathway
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Author:
Li Ai, Yongxia Li, Zhijuan Liu, Ran Li, Xiaona Wang, Bing Hai
IF:
1.6000
Publish_to:
CELLULAR AND MOLECULAR BIOLOGY
PMID:
38158661
Astragaloside IV Inhibits the Pyroptosis in the Acute Kidney Injury through Targeting the SIRT1/FOXO3a Axis
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Author:
Chengxi Zha, Yaya Qi, Fujun Xing, Jiansheng Li
IF:
1.5000
Publish_to:
CHEMICAL & PHARMACEUTICAL BULLETIN
PMID:
39477483
Correlation between periodontal status, whole salivary interleukin-1beta levels and oral yeasts carriage among individuals with varying ranges of body mass index
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Author:
Dena Ali, Toshinari Mikami, Fatema Alkazemi
IF:
1.4000
Publish_to:
ACTA ODONTOLOGICA SCANDINAVICA
PMID:
40072290
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