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Calcein，AM 钙黄绿素乙酰甲酯
英文名称:Calcein，AM
CAS:No.148504-34-1
分子式:C46H46N2O23
分子量:994.86
储存条件:-20℃干燥避光保存，保质期1年。
产品简介
| 中文名称 | Calcein，AM 钙黄绿素乙酰甲酯 |
| 英文名称 | Calcein，AM |
| 别名 | 钙黄绿素乙酰甲酯 |
| CAS | No.148504-34-1 |
| 分子式 | C46H46N2O23 |
| 分子量 | 994.86 |
| 纯度/级别 | ≥90% |
| 外观(性状) | 白色或浅黄色晶体 |
| 储存条件 | -20℃干燥避光保存，保质期1年。 |
| 规格 | 1mg |
| 单位 | 瓶 |
Calcein, AM是一种可对活细胞进行荧光标记的细胞染色试剂，它穿透细胞膜进入细胞后被细胞内的酯酶剪切形成Calcein，从而被滞留在细胞内，发出强绿色荧光。与其它同类试剂（如 BCECF, AM和CFDA）相比，Calcein, AM的细胞毒性很低。Calcein的激发和发射波长分别为 490 nm和515 nm。
Calcein, AM仅对活细胞染色。作为核染色染料的PI 不能穿过活细胞的细胞膜，它穿过死细胞膜的无序区域而到达细胞核，并嵌入细胞的 DNA双螺旋从而产生红色荧光（激发：535 nm，发射：617 nm），因此 PI 仅对死细胞染色。由于 Calcein和PI-DNA 都可被490 nm激发，因此可用荧光显微镜同时观察活细胞和死细胞。用545 nm激发，仅可观察到死细胞。根据以上特点，Calcein, AM和PI 经常被结合用来作为活细胞和死细胞的双重染色。
由于不同细胞系的最佳染色条件不同，我们建议个别确定Calcein, AM 和PI的合适浓度。
操作说明：
（1）用DMSO 制备1 mM 的Calcein, AM溶液，并用PBS 将其稀释制成1～50μM的Calcein, AM溶液①。
（2）将1/10 细胞培养基体积的Calcein, AM溶液加入到细胞培养基中②。
（3）在37℃培养细胞15～30分钟。
（4）用PBS 或适当的缓冲液洗涤细胞两次。
（5）用490 nm激发波长，515 nm发射波长的滤光片的荧光显微镜观察细胞。
注意：
如果Calcein, AM很难进入细胞，可以使用表面活性剂，如Pluronic F127 。
也可以用1/10 浓度的Calcein, AM溶液代替培养基。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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A novel multifunctional platform based on ITO/APTES/ErGO/AuNPs for long-term cell culture and real-time biomolecule monitoring
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Author：
Peng Zhao, Sha Chen, Mei Yang, Yongzhong Wang, Huibo Luo, Danqun Huo, Zhong Ji, Changjun Hou
IF：
6.0570
Publish_to：
TALANTA
PMID：
33773736
Dual drug-loaded PLGA fibrous scaffolds for effective treatment of breast cancer in situ
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Author：
Hao Chen, Jiaen Wu, Muhammad Saif Ur Rahman, Shengmei Li, Jie Wang, Shilin Li, Yan Wu, Ying Liu, Shanshan Xu
IF：
6.0000
Publish_to：
Biomaterials Advances
PMID：
36878024
Zn2+-interference and H2S-mediated gas therapy based on ZnS-tannic acid nanoparticles synergistic enhancement of cell apoptosis for specific treatment of prostate cancer
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Author：
Bo Zhou, Ben-Xu Jia, Ming-Jin Zhang, Yan-Jun Tan, Wei-Yuan Liang, Xiang Gan, Hong-Tao Li, Xiaoli Yang, Xing-Can Shen
IF：
5.9990
Publish_to：
COLLOIDS AND SURFACES B-BIOINTERFACES
PMID：
37075522
10Boron-doped carbon nanoparticles as a delivery platform for boron neutron capture therapy and photothermal therapy
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Author：
Jiacheng Li, Ziteng Chen, Zhijie Wang, Linwen Lv, Qiuyang Liu, Ya-Nan Chang, Kui Chen, Gengmei Xing
IF：
5.8000
Publish_to：
Biomaterials Science
PMID：
40302467
RGD Peptide Modified Erythrocyte Membrane/Porous Nanoparticles Loading Mir-137 for NIR-Stimulated Theranostics of Glioblastomas
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Author：
Minghui Li, Xinyu Cui, Feng Wei, Chao Li, Xiaojun Han
IF：
5.7190
Publish_to：
Nanomaterials
PMID：
35564173
Preparation of fish decalcified bone matrix and its bone repair effect in rats
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Author：
Liu Zichao, Jiang Xiaorui, Wang Kai, Zhou Yongshun, Li Tingting, Gao Jianfeng, Wang Lei
IF：
5.7000
Publish_to：
Frontiers in Bioengineering and Biotechnology
PMID：
36860886
Multifunctional phototheranostic agent ZnO@Ag for anti-infection through photothermal/photodynamic therapy
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Author：
Obeng Enoch, Feng Jiayao, Wang Danyan, Zheng Dongyang, Xiang Bailin, Shen Jianliang
IF：
5.5450
Publish_to：
Frontiers in Chemistry
PMID：
36438866
Multi-applications of carbon dots and polydopamine-coated carbon dots for Fe3+ detection, bioimaging, dopamine assay and photothermal therapy
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Author：
Chen Jun, Wang Yuting, Wang Liang, Liu Mingjie, Fang Linlin, Chu Peng, Gao Chuanzhou, Chen Dapeng, Ren Dongze, Zhang Jianbin
IF：
5.4180
Publish_to：
Nanoscale Research Letters
PMID：
36862234
Alleviation of endothelial dysfunction of Pheretima guillemi (Michaelsen)-derived protein DPf3 in ponatinib-induced thrombotic zebrafish and mechanisms explored through ox-LDL-induced HUVECs and TMT-based proteomics
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Author：
Yali Wu, Yunnan Ma, Wanling Zhong, Huijuan Shen, Jinhong Ye, Shouying Du, Pengyue Li
IF：
5.4000
Publish_to：
JOURNAL OF ETHNOPHARMACOLOGY
PMID：
38159828
Matrix Stiffness Potentiates Stemness of Liver Cancer Stem Cells Possibly via the Yes-Associated Protein Signal
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Author：
Hong Li, Yuchuan Sun, Qing Li, Qing Luo, Guanbin Song
IF：
5.3950
Publish_to：
ACS Biomaterials Science & Engineering
PMID：
35084830
A self-assembly enzyme-like hydrogel with ROS scavenging and immunomodulatory capability for microenvironment-responsive wound healing acceleration
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Author：
Xiaohui Jia, Yuhe Dong, Jihui Lu, Zhenyuan Yang, Ran Xu, Xiang Zhang, Jingyi Jiao, Zixuan Zhang, Yixuan Lin, Fuhao Chu, Penglong Wang, Tian Zhong, Haimin Lei
IF：
5.3000
Publish_to：
INTERNATIONAL JOURNAL OF PHARMACEUTICS
PMID：
40158760
Osteogenic Property Regulation of Stem Cells by a Hydroxyapatite 3D-Hybrid Scaffold With Cancellous Bone Structure
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Author：
He Xia, Lun Dong, Min Hao, Yuan Wei, Jiazhi Duan, Xin Chen, Liyang Yu, Haijun Li, Yuanhua Sang, Hong Liu
IF：
5.2210
Publish_to：
Frontiers in Chemistry
PMID：
34869241
Mitochonic acid 5 regulates mitofusin 2 to protect microglia
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Author：
Jian Tan, Shuang-Xi Chen, Qing-Yun Lei, Shan-Qing Yi, Na Wu, Yi-Lin Wang, Zi-Jian Xiao, Heng Wu
IF：
5.1350
Publish_to：
Neural Regeneration Research
PMID：
33510088
Efficient synthesis and anticancer evaluation of spider toxin peptide LVTX-8-based analogues with enhanced stability
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Author：
Qiao-Na Chi, Shi-Xi Jia, Hao Yin, Li-E Wang, Xing-Yan Fu, Yan-Nan Ma, Ming-Pu Sun, Yun-Kun Qi, Zhibo Li, Shan-Shan Du
IF：
5.1000
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BIOORGANIC CHEMISTRY
PMID：
36907048
Red blood cell membrane-functionalized Nanofibrous tubes for small-diameter vascular grafts
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Author：
Yue Jiang, Shanzhu Guo, Jingjing Jiao, Long Li
IF：
5.0000
Publish_to：
Polymers
PMID：
37376390
DPHC from Alpinia officinarum Hance specifically modulates the function of CENPU in the cell cycle and apoptosis to ameliorate hepatocellular carcinoma
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Author：
Zhe Zhu, Xiuxia Lian, Jicheng Hu, Zhe Wang, Yinghong Zhong, Yuan Zhao, Lu Lu, Yipeng Pan, Mingyan Zhou, Jian Xu
IF：
4.8000
Publish_to：
JOURNAL OF ETHNOPHARMACOLOGY
PMID：
40058474
Arctigenin Attenuates Vascular Inflammation Induced by High Salt through TMEM16A/ESM1/VCAM-1 Pathway
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Author：
Mengying Zeng, Ziyan Xie, Jiahao Zhang, Shicheng Li, Yanxiang Wu, Xiaowei Yan
IF：
4.7570
Publish_to：
Biomedicines
PMID：
36359280
Targeted Delivery of Doxorubicin Using Transferrin-Conjugated Carbon Dots for Cancer Therapy
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Author：
Lihong Li, Qi Zhang, Jinyao Li, Yafei Tian, Yu Kang, Guodong Ren, Wen Liu, Haojiang Wang, Bin Wang, Lili Yan, Lixia Guo, Haipeng Diao
IF：
4.7000
Publish_to：
ACS Applied Bio Materials
PMID：
35006957
U-Shaped Conjugated Oligomer-Based Micelles as a Ratiometric Fluorescent Nanothermometer for Diverse Cellular Applications
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Author：
Liu Xi, Zhao Yu, Niu Yangyang, Xie Qionghong, Nie Hao, Jin Yun, Zhang Yingying, Lu Yuqiu, Zhu Saiya, Zuo Wei, Yu Chen
IF：
4.6000
Publish_to：
Clinical Kidney Journal
PMID：
Guluronic acid can inhibit copper(II) and amyloid β peptide coordination and reduce copper-related reactive oxygen species formation associated with Alzheimers disease
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Author：
Hangyu Shen, Yun Dou, Xiaoying Wang, Xiaohui Wang, Fangong Kong, Shoujuan Wang
IF：
4.3360
Publish_to：
JOURNAL OF INORGANIC BIOCHEMISTRY
PMID：
37207465
Application of polydopamine-modified triphasic PLA/PCL-PLGA/Mg(OH)2-velvet antler polypeptides scaffold loaded with fibrocartilage stem cells for the repair of osteochondral defects
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Author：
Cheng Renyi, Xie Tao, Ma Wen, Deng Peishen, Liu Chaofeng, Hong Yuchen, Liu Changyu, Tian Jinjun, Xu Yanhua
IF：
4.3000
Publish_to：
Frontiers in Bioengineering and Biotechnology
PMID：
39372430
A novel protoapigenone analog RY10-4 induces apoptosis of breast cancer cells by exacerbating mitochondrial Ca2+ influx through mitochondrial calcium uniporter
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Author：
Pingping Xue, Qian Chen, Xiuhua Ren, Dong Liu, Xiaofan Yang
IF：
4.2190
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TOXICOLOGY AND APPLIED PHARMACOLOGY
PMID：
34717963
A carboxylesterase-activatable near-infrared phototheranostic probe for tumor fluorescence imaging and photodynamic therapy
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Author：
Lihong Li, Qi Zhang, Jiaojiao Li, Yafei Tian, Jinyao Li, Wen Liu, Haipeng Diao
IF：
4.0360
Publish_to：
RSC Advances
PMID：
36540215
Functional modification and antibacterial evaluation of orthodontic adhesives with poly (lysine)-derived carbon dots
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Author：
Linlin Xu, Qianqian Zhang, Yongzhi Xu, Xuecheng Xu, Mingchang Hu, Jidong Xu, Yu Song, Yuanping Hao
IF：
3.9000
Publish_to：
RSC Advances
PMID：
39980985
The effects of YKL-40 on angiogenic potential of HUVECs are partly mediated by syndecan-4
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Author：
WeiJun Sun, Qi Xue, Yan Zhao, Jianlei Zheng
IF：
3.7380
Publish_to：
International Journal of Medical Sciences
PMID：
34790051
Enhanced Bone Regeneration through Regulation of Mechanoresponsive FAK-ERK1/2 Signaling by ZINC40099027 during Distraction Osteogenesis
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Author：
Shanyu Li, Hongxiao Wu, Feng Wang, Lingchi Kong, Yifan Yu, Rongtai Zuo, Haoyu Zhao, Jia Xu, Qinglin Kang
IF：
3.6000
Publish_to：
International Journal of Medical Sciences
PMID：
38164350
Mechanisms of peripheral neurotoxicity associated with four chemotherapy drugs using human induced pluripotent stem cell-derived peripheral neurons
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Author：
Meiting Wang, Jiaxian Wang, Alex Y.P. Tsui, Zhaomin Li, Yizhe Zhang, Qi Zhao, Hongyan Xing, Xijie Wang
IF：
3.5000
Publish_to：
TOXICOLOGY IN VITRO
PMID：
34390763
Ru(II) Complexes with Enaminone Structures for Rapid Sterilization of Staphylococcus aureus and MRSA with Little Accumulation of Drug Resistance
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Author：
Yao Jian, Yatong Peng, Wanpeng Zhou, Yunli Xu, Chao Li, Xuesong Wang, Qianxiong Zhou
IF：
3.4000
Publish_to：
ChemMedChem
PMID：
36751034
Effects and mechanisms of puerarin against neuroblastoma: insights from bioinformatics and in vitro experiments
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Author：
Sui Xiaohui, Liu Tingting, Zou Zhiyun, Zhang Baoqing, Zhang Guiju
IF：
3.3000
Publish_to：
BMC Complementary Medicine and Therapies
PMID：
38982456
Regulation of Atherosclerosis by Toll-Like Receptor 4 Induced by Serum Amyloid 1: A Systematic In Vitro Study
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Author：
Jinhui Chen, Gang Liu, Yan Hong, Jing Han, Zhe Yang, Yanping Yang, Hong Li, Shumin Wang, Lili Jue, Qi Wang
IF：
3.2460
Publish_to：
Biomed Research International
PMID：
36158875
Additive Manufacturing of Porous Silicon Nitride Inspired by Triply Periodic Minimal Surface
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Author：
Shengwu Huang, Ping Yang, Haidong Wu, Shanghua Wu
IF：
3.1000
Publish_to：
3D Printing and Additive Manufacturing
PMID：
A Novel SimpleDrop Chip for 3D Spheroid Formation and Anti-Cancer Drug Assay
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Author：
Xiaoli Liu, Huichao Lin, Jiaao Song, Taiyi Zhang, Xiaoying Wang, Xiaowen Huang, Chengyun Zheng
IF：
2.8910
Publish_to：
Micromachines
PMID：
34200752
PGC1α enhances macrophage efferocytosis in ox-LDL-stimulated RAW264.7 cells by regulating the NLRP3/PPARα axis
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Author：
Qi Lu, Xujiao Chen, Qijun Zhang
IF：
2.7000
Publish_to：
TISSUE & CELL
PMID：
39047550
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