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线粒体膜电位检测试剂盒(JC-10)
英文名称:Mitochondrial Membrane Potential Kit(JC-10 Assay)
储存条件:-20℃，避光，有效期1年
产品简介
| 英文名称 | Mitochondrial Membrane Potential Kit(JC-10 Assay) |
| 中文名称 | 线粒体膜电位检测试剂盒(JC-10) |
| 储存条件 | -20℃，避光，有效期1年 |
| 运输条件 | 冷冻运输 |
| 规格 | 100T |
| 单位 | 盒 |
线粒体膜电位检测试剂盒（JC-10 ）是一种以JC-10 为荧光探针，快速灵敏地检测细胞、组织或纯化的线粒体膜电位变化的试剂盒，可以用于早期的细胞凋亡检测。
JC-10 是一种广泛用于检测线粒体膜电位△Ψm 的理想荧光探针。可以检测细胞、组织或纯化的线粒体膜电位。在线粒体膜电位较高时，JC-10 聚集在线粒体的基质中，形成聚合物，可以产生红色荧光；在线粒体膜电位较低时，JC-10 不能聚集在线粒体的基质中，此时 JC-10 为单体，可以产生绿色荧光。这样就可以非常方便地通过荧光颜色的转变来检测线粒体膜电位的变化。常用红绿荧光的相对比例来衡量线粒体去极化的比例。
线粒体膜电位的下降是细胞凋亡早期的一个标志性事件。通过 JC-10 从红色荧光到绿色荧光的转变可以很容易地检测到细胞膜电位的下降，同时也可以用JC-10 从红色荧光到绿色荧光的转变作为细胞凋亡早期的一个检测指标。
JC-10 单体的最大激发波长为 515nm ，最大发射波长为529nm ；JC-10 聚合物的最大激发波长为585nm ，最大发射波长为590nm 。实际观察时，使用常规的观察红色荧光和绿色荧光的设置即可。
本试剂盒提供了CCCP 作为诱导线粒体膜电位下降的阳性对照。对于六孔板中的样品，本试剂盒共可以检测 100 个样品；对于 12 孔中的样品，本试剂盒共可以检测200 个样品。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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New insights into Microalgal astaxanthin's effect on Lambda-cyhalothrin-induced lymphocytes immunotoxicity in Cyprinus carpio: Involving miRNA-194-5p-FoxO1-mediated-mitophagy and pyroptosis
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Author：
Jingzeng Cai, Haoyue Guan, Di Li, Bendong Shi, Yangyang Jiang, Senqiu Qiao, Qiaohan Liu, Cheng Fang, Ziwei Zhang
IF：
4.7000
Publish_to：
FISH & SHELLFISH IMMUNOLOGY
PMID：
37661035
Injectable and Degradable POSS–Polyphosphate–Polysaccharide Hybrid Hydrogel Scaffold for Cartilage Regeneration
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Author：
Xiongxiong Liu, Ting Zhao, Zheng Shi, Cuilan Hu, Qiang Li, Chao Sun
IF：
4.6000
Publish_to：
MOLECULES
PMID：
37446609
Matrine Protects Cardiomyocytes Against Hyperglycemic Stress by Promoting Mitofusin 2-Induced Mitochondrial Fusion.
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Author：
Tong Xiao, Jie Huang, Yuan Liu, Yujie Zhao, Manman Wei
IF：
4.5660
Publish_to：
Frontiers in Physiology
PMID：
33613300
Licoflavone A Suppresses Gastric Cancer Growth and Metastasis by Blocking the VEGFR-2 Signaling Pathway
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Author：
Gong Hongxia, Jin Xiaojie, Leng Guangxian, Zhang Min, Niu Shiwei, Cao Wangjie, Zhang Han, Zeng Yuanding, Li Chenghao, Li Yaling, Su Yun, Liu Yongqi
IF：
4.5010
Publish_to：
Journal of Oncology
PMID：
35509849
CC-115 Mediates GSDME-Dependent Pyroptosis in Lung Adenocarcinoma Through the Akt/Bax Pathway
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Author：
Zhang Ting, Liu Ming-Quan, Xie Guang-Su, Wu Dong-Ming, Luo Peng-Wei, Liu Teng, Deng Shi-Hua, Wang Yuan-Yi, He Shuang, Zhou Ye, Zhou Jin, Xu Ying
IF：
4.4780
Publish_to：
Journal of Cancer
PMID：
37283803
Duck Circovirus genotype 2 ORF3 protein induces apoptosis through the mitochondrial pathway
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Author：
Juan Huang, Yanting Zhang, Anchun Cheng, Mingshu Wang, Mafeng Liu, Dekang Zhu, Shun Chen, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Xumin Ou, Sai Mao, Qun Gao, Di Sun, Bin Tian, Zhongqiong Yin, Renyong Jia
IF：
4.4000
Publish_to：
POULTRY SCIENCE
PMID：
36848756
SYP-3343 drives abnormal vascularization in zebrafish through regulating endothelial cell behavior
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Author：
Guoliang Chen, Juan Liu, Huiyun Wang, Mingxing Wang, Guixue Wang, Tingzhang Hu
IF：
4.3000
Publish_to：
FOOD AND CHEMICAL TOXICOLOGY
PMID：
36796616
Mitochondria play an important role in the cell proliferation suppressing activity of berberine
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Author：
Xiao-Jin Yan, Xuan Yu, Xin-Pei Wang, Jing-Fei Jian
IF：
4.2590
Publish_to：
Scientific Reports 7, Article number: 41712 (2017)
PMID：
28181523
Microalgal astaxanthin ameliorates cypermethrin-induced necroptosis and inflammation via targeting mitochondrial Ca2+ homeostasis and the ROS-NF-κB-RIPK3/MLKL axis in carp hepatocytes (Cyprinus carpio)
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Author：
Shuoyue Li, Yinuo Liu, Di Li, Kaixuan Zhang, Zequn Zhang, Ziwei Zhang, Jingzeng Cai
IF：
4.1000
Publish_to：
FISH & SHELLFISH IMMUNOLOGY
PMID：
39370019
The structural protein VP3 of enterovirus D68 interacts with MAVS to inhibit the NF-κB signaling pathway
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Author：
Honghua Chen, Mengqian Huang, Bei Hou, Zixiang Liu, Ruyang Tan, Luna Cui, Tao Wang, Zhiyun Wang
IF：
4.0000
Publish_to：
JOURNAL OF VIROLOGY
PMID：
40042308
Photobiomodulation therapy mitigates depressive-like behaviors by remodeling synaptic links and mitochondrial function
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Author：
Hongli Chen, Xinyu Shi, Na Liu, Zhongdi Jiang, Chunyan Ma, Guoshuai Luo, Shuang Liu, Xunbin Wei, Yi Liu, Dong Ming
IF：
3.9000
Publish_to：
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
PMID：
39096719
Ciclopirox platinum(IV) conjugates suppress tumors by promoting mitophagy and provoking immune responses
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Author：
Suying Li, Shuaiqi Feng, Yan Chen, Bin Sun, Ning Zhang, Yanna Zhao, Jun Han, Zhifang Liu, Yan-Qin He, Qingpeng Wang
IF：
3.8000
Publish_to：
JOURNAL OF INORGANIC BIOCHEMISTRY
PMID：
39142055
Baicalin improves the energy levels in the prefrontal cortex of mice exposed to chronic unpredictable mild stress
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Author：
Shuaifei Lu, Caiyin Li, Xiaohui Jin, Leilei Zhu, Jiduo Shen, Ming Bai, Yucheng Li, Erping Xu
IF：
3.7760
Publish_to：
Heliyon
PMID：
36531636
Regulatory effects of Trichinella spiralis serpin-type serine protease inhibitor on endoplasmic reticulum stress and oxidative stress in host intestinal epithelial cells
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Author：
Zhen Jingbo, Lin Lihao, Li Zhixin, Sun Feng, Han Yang, Li Qiankun, Yang Yuqi, Liu Xueting, Yu Junchen, Zhang Qi, Lu Yixin, Han Caixia
IF：
3.7000
Publish_to：
VETERINARY RESEARCH
PMID：
38877574
Tandem mass tag-based quantitative proteomics analysis reveals the effects of the α-lactalbumin peptides GINY and DQW on lipid deposition and oxidative stress in HepG2 cells
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Author：
Haoran Chen, Xiaofen Qi, Kaifang Guan, Rongchun Wang, Qiming Li, Ying Ma
IF：
3.5000
Publish_to：
JOURNAL OF DAIRY SCIENCE
PMID：
36797178
Tetramethylpyrazine induces differentiation of human umbilical cord-derived mesenchymal stem cells into neuron-like cells in vitro
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Author：
Chengrui Nan,Li Guo,Zongmao Zhao,Shucheng Ma,Jixia
IF：
3.3330
Publish_to：
International
Journal of
Oncology
PMID：
27035275
CD137 Signal Mediates Cardiac Ischemia–Reperfusion Injury by Regulating the Necrosis of Cardiomyocytes
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Author：
Xu Yao, Chen Rui, Yan Jinchuan, Zang Guangyao, Shao Chen, Wang Zhongqun
IF：
3.2160
Publish_to：
Journal of Cardiovascular Translational Research
PMID：
35419772
Protective Role of Oxycodone in Myocardial Oxidative Stress and Mitochondrial Dysfunction Induced by Ischemia-Reperfusion
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Author：
Yongzheng Jiang, Hua He, Xinwei Jia
IF：
3.2000
Publish_to：
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
PMID：
39865943
Eugenol protects chondrocytes and articular cartilage by downregulating the JAK3/STAT4 signaling pathway
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Author：
Zhimin Wu, Ying Wang, Guoqiang Yan, Chengai Wu
IF：
3.1020
Publish_to：
JOURNAL OF ORTHOPAEDIC RESEARCH
PMID：
35880357
Supplementation of Schisandrin B in Semen Extender Improves Quality and Oxidation Resistance of Boar Spermatozoa Stored at 4 °C
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Author：
Yunfa Xie, Zhiying Chen, Yanling Wang, Xiayun Peng, Ni Feng, Xiaoye Wang, Yinsheng Tang, Xun Li, Chunrong Xu, Chuanhuo Hu
IF：
3.0000
Publish_to：
Animals
PMID：
36899705
HIF‑1α protein SUMOylation is an important protective mechanism of action of hypothermia in hypoxic cardiomyocytes
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Author：
Huaqin Liu, Xiyun Bian, Meili Xu, Xiaofang Ma, Chunyan Zhang, Jinjin Jiang, Jianfeng Fu, Xiaozhi Liu
IF：
2.9520
Publish_to：
Molecular Medicine Reports
PMID：
33899119
Type of the study: Research article effect of melatonin on cryopreservation of Beijing you chicken (gallus gallus) spermatozoa
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Author：
Laiqing Yan, Junying Li, Guangdong Li, Wenkui Ma, Yunjie Liu, Xuening Liu, Mengmeng Zhao, Pengyun Ji, Yao fu, Qihao Zheng, Xuemei Deng, Guoshi Liu
IF：
2.7000
Publish_to：
CRYOBIOLOGY
PMID：
37981093
Synergistic inhibitory effect of atmospheric pressure plasma and berberine on non‑small cell lung cancer cells via inducing apoptosis
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Author：
Ming Huiyun, Lu Tingting, Zhou Han, Song Wencheng, Dai Haiming
IF：
2.6000
Publish_to：
MOLECULAR BIOLOGY REPORTS
PMID：
39643828
PrG Protects Postovulatory Oocytes Aging in Mice through the Putrescine Pathway
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Author：
Ronghua Ma, Xi Zhao, Jing Zhao, Yi Yi, Shengyan Jian, Xueman Ma, Zhanhai Su
IF：
2.5000
Publish_to：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
PMID：
39053107
Licorice protects against ulcerative colitis via the Nrf2/PINK1-mediated mitochondrial autophagy
点击查看 >>
Author：
Jinrong Kong, Qingzhen Xiang, Gaoxiang Shi, Zaiping Xu, Xiaowen Ma, Yunlai Wang, Zihua Xuan, Fan Xu
IF：
2.4930
Publish_to：
Immunity Inflammation and Disease
PMID：
36705402
Microalgae Octapeptide IIAVEAGC Alleviates Oxidative Stress and Neurotoxicity in 6-OHDA-Induced SH-SY5Y Cells by Regulating the Nrf2/HO-1 and Jak2/Stat3 Pathways
点击查看 >>
Author：
Yi Liu, Liyuan Lin, Haiyan Zheng, Huixue Huang, Zhong-Ji Qian
IF：
2.3000
Publish_to：
CHEMISTRY & BIODIVERSITY
PMID：
38594219
Combining the Novel FLT3 and MERTK Dual Inhibitor MRX-2843 with Venetoclax Results in Promising Antileukemic Activity Against FLT3-ITD AML
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Author：
Shuangshuang Wu, Fangbing Liu, Yuqing Gai, Jenna Carter, Holly Edwards, Maik Hüttemann, Guan Wang, Chunhuai Li, Jeffrey W. Taub, Yue Wang, Yubin Ge
IF：
2.1000
Publish_to：
LEUKEMIA RESEARCH
PMID：
38968731
Inhibition of Neospora caninum activity by niclosamide: Evidence from in vitro and in vivo studies
点击查看 >>
Author：
Feixue Liu, Xin Li, Liuzhenxiu Yan, Xu Zhang, Jin Sun, Haitao Su, Lu Li, Sining Chen, Lanbi Gao, Pengtao Gong, Nan Zhang, Xichen Zhang, Jianhua Li, Xiaocen Wang
IF：
2.0000
Publish_to：
VETERINARY PARASITOLOGY
PMID：
39637465
Shikonofuran E plays an anti-inflammatory role by down-regulating MAPK and NF- B signaling pathways in lipopolysaccharide-stimulated RAW264.7 macrophages
点击查看 >>
Author：
Lang CaoYong XueZixiong YangYanhong LiHongmei LiXu
IF：
1.9200
Publish_to：
Journal of Natural Medicines
PMID：
30121934
PCSK7, a potential target for the treatment of age-related macular degeneration: inhibition of retinal epithelial cell death
点击查看 >>
Author：
Xiaolong Zhang, Xin Zhao, Xiangyang Xin
IF：
1.1000
Publish_to：
International Journal of Clinical and Experimental Pathology
PMID：
39544716
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