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相关信息

别名 SYBR Green II RNA
英文名称 SYBR Green II RNA
储存条件 -20°C,有效期2年
单位
规格    50ul    100ul    1ml

l Analyze small aliquots from RNA preparations before Northern blotting, start-site mapping or cDNA preparation

l Visualize the migration behavior of 5S rRNA species after high-resolution denaturing gradient electrophoresis (DGGE)

l Stain DNA in single-strand conformation polymorphism (SSCP)

l Stain DNA before amplification by PCR

Materials Contents

SYBR Green II stain is provided as a 10,000X concentrate in DMSO in the following sizes:

l 50 μL (SY1040-50)

l 500 μL (SY1040-500)

Storage

Upon receipt, store the dye frozen at -20°C, protected from light in a desiccator. When stored properly, the SYBR Green II stain stock solution in DMSO is stable for six months to one year.

注:SYBR Green II主要用于RNA和单链DNA电泳。是EB的替代品,无致癌性,超敏感性。用作常规电泳时,须将商品化的核酸MARKER作一定倍数的稀释(1/5-1/10),这样才能得到比较理想的结果。



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