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Hoechst 33342 染色液(10ug/ml，即用型)
英文名称:Hoechst 33342 Stain solution(ready-to-use)
储存条件:-20℃，避光，有效期1年。
产品简介
| 中文名称 | Hoechst 33342 染色液(10ug/ml，即用型) |
| 英文名称 | Hoechst 33342 Stain solution(ready-to-use) |
| 别名 | bisBenzimide H 33342;HOE 33342 |
| 储存条件 | -20℃，避光，有效期1年。 |
| 规格 | 10ml 50ml |
| 单位 | 瓶 |
Hoechst 33342，也称bisBenzimide H 33342或HOE 33342，是一种可以穿透细胞膜的蓝色荧光染料，对细胞的毒性较低。Hoechst 33342染色常用于细胞凋亡检测，染色后用荧光显微镜观察或流式细胞仪检测。Hoechst 33342也常用于普通的细胞核染色，或常规的DNA染色。Hoechst 33342的最大激发波长为346nm，最大发射波长为460nm；Hoechst 33342和双链DNA结合后，最大激发波长为350nm，最大发射波长为461nm。本Hoechst 33342染色液为即用型，可直接用于固定细胞或组织的细胞核染色，也可直接用于活细胞或组织的细胞核染色。
使用说明：
1. 对于固定的细胞或组织：
a. 对于细胞或组织样品，固定后，适当洗涤去除固定剂。随后如果需要进行免疫荧光染色，则先进行免疫荧光染色，染色完毕后再按后续步骤进行Hoechst 33342染色。如果不需要进行其它染色，则直接进行后续的Hoechst 33342染色。
b. 对于贴壁细胞或组织切片，加入少量Hoechst 33342染色液，覆盖住样品即可；对于悬浮细胞，至少加入待染色样品3倍体积的染色液，混匀。室温放置3-5分钟。
c. 吸除Hoechst 33342染色液，用TBST、PBS或生理盐水洗涤2-3次，每次3-5分钟。
d. 直接在荧光显微镜下观察或封片后荧光显微镜下观察。细胞发生凋亡时，会看到凋亡细胞的细胞核呈致密浓染，或呈碎块状致密浓染。
2. 对于活细胞或组织：
a. 加入适当量Hoechst 33342染色液，必须充分覆盖住待染色的样品，通常对于六孔板一个孔需加入1ml染色液，对于96孔板一个孔需加入100微升染色液。
b. 在适宜于细胞培养的温度下培养20-30分钟。弃染色液，用PBS或培养液洗涤2-3次即可进行荧光检测。
注意事项：
荧光染料都存在淬灭的问题，为减缓荧光淬灭可以使用抗荧光衰减封片剂。建议染色后尽量当天完成检测，活细胞或组织染色后应立即观察。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
PMID：
40017402
The potential role of the HCN1 ion channel and BDNF-mTOR signaling pathways and synaptic transmission in the alleviation of PTSD
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Author：
倪良卉
IF：
5.6810
Publish_to：
PMID：
32198387
Ahf-Caltide, a Novel Polypeptide Derived from Calpastatin, Protects against Oxidative Stress Injury by Stabilizing the Expression of CaV1.2 Calcium Channel
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Author：
Yingchun Xue, Shi Zhou, Ling Yan, Yuelin Li, Xingrong Xu, Xianghui Wang, Etsuko Minobe, Masaki Kameyama, Liying Hao, Huiyuan Hu
IF：
5.6000
Publish_to：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
PMID：
37958713
Co-immunization with DNA vaccines encoding yidR and IL-17 augments host immune response against Klebsiella pneumoniae infection in mouse model
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Author：
Zheng Lv, Xuan Zhang, Kelei Zhao, Lianming Du, Xinrong Wang, Yiwen Chu, Ting Huang
IF：
5.5000
Publish_to：
Virulence
PMID：
38656137
Developing a Selection Framework for Zinc Ion-Based Biomaterial Design: Guided by the Biosafety Assessment of ZIF-8 and ZnO
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Author：
Yilin Mao, Liang Wang, Zhengyi Xu, Wenjia Xie, Yuting Wang, Wei Qiao, Zhou Zhu, Jian Wang
IF：
5.4000
Publish_to：
ACS Biomaterials Science & Engineering
PMID：
38632925
Microfluidic Formulation of Curcumin-Loaded Multiresponsive Gelatin Nanoparticles for Anticancer Therapy
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Author：
Yu Xia, Ruicheng Xu, Siyuan Ye, Jiaxuan Yan, Piyush Kumar, Peng Zhang, Xiubo Zhao
IF：
5.3950
Publish_to：
ACS Biomaterials Science & Engineering
PMID：
37140447
Suppression of Cancer Cell Stemness and Drug Resistance via MYC Destabilization by Deubiquitinase USP45 Inhibition with a Natural Small Molecule
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Author：
Xiao Tu, Chuncheng Li, Wen Sun, Xi Tian, Qiufu Li, Shaoxin Wang, Xiaoling Ding, Zhen Huang
IF：
5.2000
Publish_to：
Cancers
PMID：
36765885
Guggulsterone from Commiphora mukul potentiates anti-glioblastoma efficacy of temozolomide in vitro and in vivo via down-regulating EGFR/PI3K/Akt signaling and NF-κB activation
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Author：
Hong-Bin Xu, Xian-Zhen Chen, Zhou-Lun Yu, Fei Xue
IF：
5.1950
Publish_to：
JOURNAL OF ETHNOPHARMACOLOGY
PMID：
36280019
Staphylococcus aureus extracellular vesicles induce apoptosis and restrain mitophagy-mediated degradation of damaged mitochondria
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Author：
Xiaozhou Wang, Hongyan Li, Jie Wang, Huiling Xu, Kun Xue, Xiaoting Liu, Zhizhong Zhang, Jianzhu Liu, Yongxia Liu
IF：
5.0700
Publish_to：
MICROBIOLOGICAL RESEARCH
PMID：
37267814
Macrocyclic polyamine [12]aneN3 modified triphenylamine-pyrazine derivatives as efficient non-viral gene vectors with AIE and two-photon imaging properties
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Author：
马乐乐
IF：
5.0470
Publish_to：
PMID：
32222754
PDE12 disrupts mitochondrial oxidative phosphorylation and mediates mitochondrial dysfunction to induce oral mucosal epithelial barrier damage in oral submucous fibrosis
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Author：
Qihui Wei, Linlin Chen, Wen Luo, Cailian Chen, Yuqing Shi, Jinmei Xie, Xi Xie, Hai-Bin Luo
IF：
5.0000
Publish_to：
EUROPEAN JOURNAL OF PHARMACOLOGY
PMID：
38325798
Degradation protection and enhanced biocompatibility of Mg alloys pretreated with plasma proteins
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Author：
Xian Wei, Jiajia Meng, Sujie Ma, Yanchun Li, Hong Qing, Xubiao Peng, Qing Zhao
IF：
4.9000
Publish_to：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
PMID：
38327244
Matrix stiffness induces an invasive-dormant subpopulation via cGAS-STING axis in oral cancer
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Author：
Li Jingyuan, Liu Yu, Jiang Hong, Wang Tao, Li Kan, Lao Xiaomei, Liao Guiqing, Liang Yujie
IF：
4.8030
Publish_to：
Translational Oncology
PMID：
37137218
Tacrolimus alleviates pulmonary fibrosis progression through inhibiting the activation and interaction of ILC2 and monocytes
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Author：
Bowen Liu, Qiuyan Jiang, Ruxuan Chen, Huizhe Zhang, Qin Xia, Chi Shao, Xiangning Liu, Mengqi Wang, Yujie Shi, Jingyan Zhu, Ruixi Zhao, Haixia Jiang, Shaoyan Gao, Xiaohe Li, Honggang Zhou, Cheng Yang, Hui Huang
IF：
4.8000
Publish_to：
INTERNATIONAL IMMUNOPHARMACOLOGY
PMID：
38581994
Chemopreventive Role of Apigenin against the Synergistic Carcinogenesis of Human Papillomavirus and 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone
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Author：
尹方正
IF：
4.7100
Publish_to：
PMID：
33158065
Circular RNA CircFOXO3 Functions as a Competitive Endogenous RNA for Acid-Sensing Ion Channel Subunit 1 Mediating Oxeiptosis in Nucleus Pulposus
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Author：
Xi Chen, Ying Song, Guanghui Chen, Baoliang Zhang, Yang Bai, Chuiguo Sun, Dongwei Fan, Zhongqiang Chen
IF：
4.7000
Publish_to：
Biomedicines
PMID：
38540291
Study on the Anti-demyelination Mechanism of Bu-Shen-Yi-Sui Capsule in the Central Nervous System Based on Network Pharmacology and Experimental Verification
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Author：
Zheng Zha, Yi-Jiang Liu, Si-Si Liu, Nan Zhang, Jun-Ling Li, Fang Qi, Liang-Yun Jin, Bing Xue, Tao Yang, Yong-Ping Fan, Hui Zhao, Lei Wang
IF：
4.5290
Publish_to：
MEDIATORS OF INFLAMMATION
PMID：
35865997
MLKL–Mediated Necroptosis Predominantly Contributes to Immune-Associated Myocardial Damage
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Author：
Sun Jinglei, Wu Wenting, Wang Yi, Zhang Jiali, Qiu Suhua, Guan Zhengkun, Shi Chenxia, Ma Jingtao, Xu Yanfang
IF：
4.5000
Publish_to：
INFLAMMATION
PMID：
40195182
Anthelmintic niclosamide attenuates pressure-overload induced heart failure in mice
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Author：
Yao Fu, Nan Hu, Ming Cao, Wen-Feng Li, Xin-Rui Yang, Jin-Lai Gao, Jing Zhao, Man Jiang, Ming-Hui Ma, Zhi-Jie Sun, De-Li Dong
IF：
4.4320
Publish_to：
EUROPEAN JOURNAL OF PHARMACOLOGY
PMID：
34736968
Oxidized phospholipid POVPC contributes to vascular calcification by triggering ferroptosis of vascular smooth muscle cells
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Author：
Lihe Lu, Yuanzhi Ye, Yajun Chen, Liyun Feng, Jiali Huang, Qingchun Liang, Zirong Lan, Qianqian Dong, Xiaoyu Liu, Yining Li, Xiuli Zhang, Jing-Song Ou, An Chen, Jianyun Yan
IF：
4.4000
Publish_to：
FASEB JOURNAL
PMID：
38581243
The selective NLRP3 inhibitor MCC950 hinders atherosclerosis development by attenuating inflammation and pyroptosis in macrophages
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Author：
Zeng Wenyun, Wu Danbin, Sun Yingxin, Suo Yanrong, Yu Qun, Zeng Miao, Gao Qing, Yu Bin, Jiang Xijuan, Wang Yijing
IF：
4.3800
Publish_to：
Scientific Reports
PMID：
34588488
Resveratrol Upregulates miR‐124‐3p Expression to Target DAPK1, Regulating the NLRP3/Caspase‐1/GSDMD Pathway to Inhibit Pyroptosis and Alleviate Spinal Cord Injury
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Author：
Daohui Li, Yongwen Dai, Zhengtao Li, Hangchuan Bi, Haotian Li, Yongquan Wang, Yuan Liu, Xinpeng Tian, Lingqiang Chen
IF：
4.3000
Publish_to：
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
PMID：
39833100
Chemical mitochondrial uncouplers share common inhibitory effect on NLRP3 inflammasome activation through inhibiting NFκB nuclear translocation
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Author：
Nan Hu, Yao Fu, Wen-Feng Li, Xin-Rui Yang, Ming Cao, Feng-Feng Li, Jia-Hui Chen, Xu-Yang Chen, Hui Zhao, Zhi-Jie Sun, De-Li Dong
IF：
4.2190
Publish_to：
TOXICOLOGY AND APPLIED PHARMACOLOGY
PMID：
33524445
HIF-1 induced tiRNA-Lys-CTT-003 is protective against cisplatin induced ferroptosis of renal tubular cells in mouse AKI model
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Author：
Dan Li, Xian Xie, Zishun Zhan, Nannan Li, Ni Yin, Shikun Yang, Jishi Liu, Jianwen Wang, Zhi Li, Bin Yi, Hao Zhang, Wei Zhang
IF：
4.2000
Publish_to：
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
PMID：
38871033
Rapeseed peptide inhibits HepG2 cell proliferation by regulating the mitochondrial and P53 signaling pathways
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Author：
Keer Ma, Zhigao Wang, Xingrong Ju, Jiankang Huang, Rong He
IF：
4.1250
Publish_to：
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
PMID：
36168817
Black carp A20 inhibits interferon signaling through de-ubiquitinating IKKβ
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Author：
Xiao Yang, Lixia Xie, Yuqi Yin, Can Yang, Jun Xiao, Hui Wu, Chanyuan Wang, Yu Tian, Hao Feng
IF：
4.1000
Publish_to：
FISH & SHELLFISH IMMUNOLOGY
PMID：
39029718
Electrical stimulation induced structural 3D human engineered neural tissue with well-developed neuronal network and functional connectivity
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Author：
Xiaoting Meng, Xiyao Yu, Yingli Lu, Zhe Pei, Guoqiang Wang, Mingran Qi, Rongrong Liu, Jiaying Zhou, Xiaopin Guo, Zhengjie Zhou, Fang Wang
IF：
4.0000
Publish_to：
Journal of Neural Engineering
PMID：
37433290
Phlorizin from sweet tea inhibits the progress of esophageal cancer by antagonizing the JAK2/STAT3 signaling pathway
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Author：
Zhenxian Jia, Yuning Xie, Hongjiao Wu, Zhuo Wang, Ang Li, Ze Li, Zhenbang Yang, Zhi Zhang, Zhaobin Xing, Xuemei Zhang
IF：
3.9060
Publish_to：
ONCOLOGY REPORTS
PMID：
34036398
Role of VEGFB in electrical pulse stimulation inhibits apoptosis in C2C12 myotubes
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Author：
LanLan Guo, YanJun Li, Zheng Xing, JingBo Zhang, Jing Zhang
IF：
3.8670
Publish_to：
PEPTIDES
PMID：
35660637
Replication of standard bovine viral diarrhea strain OregonC24Va induces endoplasmic reticulum stress–mediated apoptosis of bovine trophoblast cells
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Author：
Yuan Naihan, Song Quanjiang, Jin Yan, Zhang Zhenhao, Wu Zheng, Sheng Xihui, Qi Xiaolong, Xing Kai, Xiao Longfei, Wang Xiangguo
IF：
3.8270
Publish_to：
CELL STRESS & CHAPERONES
PMID：
36441379
Characterization and functional analysis of chicken promyelocytic leukemia protein
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Author：
Shengnan Wang, Jingwen Li, Tiantian Miao, Tuofan Li, Zhimin Wan, Quan Xie, Hongxia Shao, Aijian Qin, Jianqiang Ye
IF：
3.8000
Publish_to：
POULTRY SCIENCE
PMID：
39293264
SENP1-mediated SUMOylation of SIRT1 affects glioma development through the NF-百B pathway
点击查看 >>
Author：
Xin Liu, Shenglin Zhang, Yi Dong, Yunpeng Xie, Qingshan Li
IF：
3.7000
Publish_to：
EXPERIMENTAL CELL RESEARCH
PMID：
37866458
lncRNA NEAT1-let 7b-P21 axis mediates the proliferation of neural stem cells cultured in vitro promoted by radial extracorporeal shock wave
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Author：
Kun Han, Nan Kang, Xiaotong Yu, Jie Lu, Yuewen Ma
IF：
3.6510
Publish_to：
Regenerative Therapy
PMID：
35844294
ameliorative effect of recombinant human lactoferrin on the premature ovarian failure in rats after cyclophosphamide treatments
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Author：
Gang LIU
IF：
3.5000
Publish_to：
Journal of Ovarian Research
PMID：
33478578
Exosomal miR-155-5p Facilitates Lipopolysaccharide Transport and Foam Cell Formation: A Novel Link Between Periodontitis and Atherosclerosis
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Author：
Wen-Wen Yang, Qing-Xiang Li, Fei Wang, Xin-Ran Zhang, Xian-Li Zhang, Meng Wang, Dong Xue, Ying Zhao, Lu Tang
IF：
3.4000
Publish_to：
JOURNAL OF PERIODONTAL RESEARCH
PMID：
39604303
Etanercept Protected Against Cigarette Smoke Extract-Induced Inflammation and Apoptosis of Human Pulmonary Artery Endothelial Cells via Regulating TNFR1
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Author：
Hong Xue, Baosong Xie, Nengluan Xu, Hongru Li, Qianshun Chen, Weiping Xie, Hong Wang
IF：
3.3550
Publish_to：
International Journal of Chronic Obstructive Pulmonary Disease
PMID：
34007171
Quantitative analysis of the effects of vimentin intermediate filaments on the early stages of influenza A virus infection
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Author：
Ju-Mei Zhang, Zhi-Gang Wang, Lei Du, Dan-Dan Fu, Meng-Qian Zhang, Jing Li, Shu-Lin Liu, Dai-Wen Pang
IF：
3.3000
Publish_to：
NEW JOURNAL OF CHEMISTRY
PMID：
Inhibition of Neutrophil Extracellular Traps Formation by Cl-Amidine Alleviates Lipopolysaccharide-Induced Endometritis and Uterine Tissue Damage
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Author：
Wenxiang Shen, Ayodele Olaolu Oladejo, Xiaoyu Ma, Wei Jiang, Juanshan Zheng, Bereket Habte Imam, Shengyi Wang, Xiaohu Wu, Xuezhi Ding, Baohua Ma, Zuoting Yan
IF：
3.2310
Publish_to：
Animals
PMID：
35565576
Protective effect and mechanism of Lycium ruthenicum Murray anthocyanins against retinal damage induced by blue light exposure
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Author：
Ping Li, Zhengang Li, Qixiu Sun, Wei Zhang, Xine Huang, Mohan Si, Xinjun Du, Shuo Wang
IF：
3.2000
Publish_to：
JOURNAL OF FOOD SCIENCE
PMID：
38992868
Sequence-dependent effects of hematoporphyrin derivatives (HPD) photodynamic therapy and cisplatin on lung adenocarcinoma cells
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Author：
Nana Li, Shichao Cui, Aizhen Yang, Baohong Xiao, Yiwei Cao, Xiaohui Yang, Cunzhi Lin
IF：
3.1000
Publish_to：
Photodiagnosis and Photodynamic Therapy
PMID：
38679153
Network pharmacology and in vitro experiments reveal sophoridine-induced apoptosis and G1 phase arrest via ROS-dependent PI3K/Akt/FoxO3a pathway activation in human bladder cancer cells
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Author：
Yao Su, Lin Chen, Jin Yang
IF：
3.0000
Publish_to：
Chemical Biology & Drug Design
PMID：
38346772
ZnSO4 Protects against premature ovarian failure through PI3K/AKT/GSK3β signaling pathway
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Author：
Zhe Dong, Lu Zhang, Wei Wang, Fan Jiang, Hao Ai
IF：
2.9230
Publish_to：
THERIOGENOLOGY
PMID：
37269597
Protective Effect of Cannabidiol on Hydrogen Peroxide-Induced Oxidative Damage in Human Umbilical Vein Endothelial Cells (HUVECs)
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Author：
Hang Li, Tian-Xiao Yang, Qing-Sheng Zhao, Bing Zhao
IF：
2.9000
Publish_to：
CHEMISTRY & BIODIVERSITY
PMID：
37382028
Downregulation of circular RNA hsa_circ_0087856 sensitizes bladder cancer cells to cisplatin through targeting miR-1184/CITED2 signaling
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Author：
Min Ju, Weiwei Wu, Jingkun Qu, Yang Sun, Jun Li
IF：
2.8000
Publish_to：
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS
PMID：
37392146
LncRNA MALAT1 Regulates USP22 Expression Through EZH2-Mediated H3K27me3 Modification to Accentuate Sepsis-Induced Myocardial Dysfunction
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Author：
Xu Hong, Ye Wei, Shi Baochang
IF：
2.7550
Publish_to：
Cardiovascular Toxicology
PMID：
35726125
LINC01936 inhibits the proliferation and metastasis of lung squamous cell carcinoma probably by EMT signaling and immune infiltration
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Author：
Qinqin Tian, Xiyao Liu, Ang Li, Hongjiao Wu, Yuning Xie, Hongmei Zhang, Fengjun Wu, Yating Chen, Congcong Bai, Xuemei Zhang
IF：
2.7000
Publish_to：
PeerJ
PMID：
38084139
MicroRNA-223 downregulation promotes HBx-induced podocyte pyroptosis by targeting the NLRP3 inflammasome
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Author：
Yu Yani, Dong Hui, Zhang Yue, Sun Jingyi, Li Baoshuang, Chen Yueqi, Feng Moxuan, Yang Xiaoqian, Gao Shengbo, Jiang Wei
IF：
2.6850
Publish_to：
ARCHIVES OF VIROLOGY
PMID：
35731327
The Role and Mechanism of Nicotinamide Riboside in Oxidative Damage and a Fibrosis Model of Trabecular Meshwork Cells
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Author：
Yuping Zeng, Yijun Lin, Juhua Yang, Xiaohui Wang, Yihua Zhu, Biting Zhou
IF：
2.6000
Publish_to：
Translational Vision Science & Technology
PMID：
38546981
Tanshinone IIa alleviates LPS-induced oxidative stress in dairy cow mammary epithelial cells by activating the Nrf2 signalling pathway
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Author：
Kaiqiang Fu, Yuning Sun, Junbo Wang, Rongfeng Cao
IF：
2.5540
Publish_to：
RESEARCH IN VETERINARY SCIENCE
PMID：
36027684
Hepatitis B virus X mediates podocyte pyroptosis by regulating the ROS/NLRP3 signaling pathway in hepatitis B virus-associated glomerulonephritis
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Author：
Yani Yu, Hui Dong, Jingyi Sun, Baoshuang Li, Yueqi Chen, Moxuan Feng, Xiaoqian Yang, Wei Jiang
IF：
2.5320
Publish_to：
Iranian Journal of Basic Medical Sciences
PMID：
35656447
In vitro induction of anti‑lung cancer immune response by the A549 lung cancer stem cell lysate‑sensitized dendritic cell vaccine
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Author：
Letian Chen, Wei Rao, Yujuan Chen, Junping Xie
IF：
2.5000
Publish_to：
Oncology Letters
PMID：
39328277
Roles of ERRα and TGF‑β signaling in stemness enhancement induced by 1 µM bisphenol A exposure via human neural stem cells
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Author：
Panpan Dong, Ganghui Ye, Xinzhuo Tu, Ying Luo, Weitong Cui, Yuxin Ma, Lei Wei, Xuewen Tian, Qinglu Wang
IF：
2.4470
Publish_to：
Experimental and Therapeutic Medicine
PMID：
35069845
Staphylococcus aureus promotes its intracellular survival by inhibiting Rab11-Rab11FIP4-mediated vesicle trafficking
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Author：
Huiling Xu, Xiaozhou Wang, Zhizhong Zhang, Jiaqing Hu, Yongtao Yu, Jiandong Wang, Yongxia Liu, Jianzhu Liu
IF：
2.4000
Publish_to：
VETERINARY MICROBIOLOGY
PMID：
38626624
Establishment of functional trophoblast organoids from trophoblast cells of bovine placenta
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Author：
Bingying Liu, Siqi Ren, Hong An, Yixuan Liang, Xihui Sheng, Xiaolong Qi, Longfei Xiao, Xiangguo Wang
IF：
2.1000
Publish_to：
Cells & Development
PMID：
39243977
Antitumor effects of a novel photosensitizer-mediated photodynamic therapy and its influence on the cell transcriptome
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Author：
JINGJING CHEN, DAN WANG, ZEQUN WANG, MENGYUAN HAN, HOUQING YIN, WENTING ZHOU, RIBAI YAN, YAN PAN
IF：
2.0000
Publish_to：
ONCOLOGY RESEARCH
PMID：
38686054
The imprinted Igf2-Igf2r axis is critical for exosome biogenesis during the early development of bovine placenta
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Author：
Kunhua ZHENG, Longfei XIAO, Naihan YUAN, Xihui SHENG, Xiaolong QI, Yingqiu WANG, Chang CHEN, Kaijun GUO, Lin YANG, Bingying LIU, Xiangguo WANG
IF：
1.9000
Publish_to：
JOURNAL OF REPRODUCTION AND DEVELOPMENT
PMID：
39662929
技术问答
（3）
注意：
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。
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6孔板接种贴壁细胞要怎么进行染色？直接将1ml染料加入到培养基中吗？2024-10-15 15:59:15
您好，去除培养基，然后用PBS清洗2次，然后加入染色液孵育8-15min，然后吸去染色液，用PBS清洗3次，最后置于荧光显微镜下观察染色结果。
该产品是无菌的嘛？2024-09-09 21:00:54
您好，不是
该试剂为10ug/ml在进行免疫荧光染色时，需要稀释嘛？2024-08-17 09:34:03
您好，不需要