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双组分TMB显色液
英文名称:TMB Two-Component Substrate solution(for Elisa)
储存条件:2-8℃，avoid light，1 year
产品简介
| 英文名称 | TMB Two-Component Substrate solution(for Elisa) |
| 中文名称 | 双组分TMB显色液 |
| 储存条件 | 2-8℃，avoid light，1 year |
| 规格 | 2*50ml 2*100ml 250ml*2 2*500ml |
| 单位 | 瓶 |
运输温度：常温运输
| 试剂名称 | 2*50mL | 2*100mL | 2*250mL | 2*500mL |
| 显色液A | 50mL | 100mL | 250mL | 500mL |
| 显色液B | 50mL | 100mL | 250mL | 500mL |
保存：2-8℃避光保存，有效期1年。
产品简介:
目前酶免疫分析（EIA)技术，已被广泛应用于抗原，半抗原或抗体的定量或定性检测分析。辣根过氧化物酶（HRP)及其偶联物是酶联免疫分析技术中常用的一种酶，由于3,3',5,5'-四甲基联苯胺（TMB)在HRP的显色反应体系中，比其它色原具有更高的灵敏度且无致癌性而被广泛应用。TMB主要应用于酶联免疫吸附实验（ELISA)，免疫斑点杂交或者免疫组化以及氯和过氧化氢的检测分析。为了满足不同的试剂盒产品研发与生产以及科研需求，本公司专门研制了针对不同类型的基于HRP的免疫分析用TMB显色液。
外观与结构：显色液A应为无色或淡黄色透明液体，显色液B应为无色透明液体。均无沉淀、颗粒及絮状物。
用于酶联免疫实验中的显色阶段：可于样本反应，生成深蓝色产物。
使用方法：
加液：待样品孔中加完HRP结合物并孵育一定时间后，用适当洗涤液洗板3-5次，每孔加底物显色液A、显色液B各50ul（A、B液先混合再加入孔中），轻轻混匀，根据个人实验需要，在室温（15-25℃）或37℃下避光温育10-30分钟或更长时间，直至显色至预期深浅。
终止：每孔加入等体积的1M 盐酸或硫酸溶液终止反应，孔中反应液由蓝色变为黄色。
读数：终止反应后15分钟内在450nm处测定各孔溶液的吸光值。
对照：空白对照不加HRP标记的抗体/抗原和阴性对照结果应无色。阳性对照及加HRP标记的抗体/抗原产生深蓝色产物。
注意：如果出现高的反应背景或沉淀，表明TMB底物反应过于强烈。为了避免产生沉淀，可在终止后马上读数；或者进一步稀释一抗和/或HRP结合物。
注意事项：
1、实验用品应该专一，避免交叉污染。
2、TMB对人体有刺激性，请注意适当防护。
3、为了您的安全和健康，请穿实验服并戴一次性手套操作
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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A fast and ultrasensitive ELISA based on rolling circle amplification
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Author：
Minli You, Ping Peng, Zhenrui Xue, Haoyang Tong, Wanghong He, Ping Mao, Qi Liu, Chunyan Yao, Feng Xu
IF：
4.6160
Publish_to：
ANALYST
PMID：
33899835
Schistosoma japonicum extracellular vesicle proteins serve as effective biomarkers for diagnosing parasite infection
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Author：
Wu Huixin, Giri Bikash R., Li Huimin, Zheng Yameng, Yan Xiaoli, Cheng Guofeng
IF：
4.6000
Publish_to：
Frontiers in Cellular and Infection Microbiology
PMID：
38817446
Screening and optimization of shark nanobodies against SARS-CoV-2 Spike RBD
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Author：
Xiaochun Liu, Yanqing Wang, Lishan Sun, Guokai Xiao, Ning Hou, Jin Chen, Wei Wang, Ximing Xu, Yuchao Gu
IF：
4.5000
Publish_to：
ANTIVIRAL RESEARCH
PMID：
38692413
Vip3Aa domain IV and V mutants confer higher insecticidal activity against Spodoptera frugiperda and Helicoverpa armigera
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Author：
Xiaoxue Yang, Zeyu Wang, Lili Geng, Baoyan Chi, Rongmei Liu, Haitao Li, Jiguo Gao, Jie Zhang
IF：
4.4620
Publish_to：
PEST MANAGEMENT SCIENCE
PMID：
35243758
Identification of nanobodies against hepatocellular carcinoma marker glypican-3
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Author：
Wenyi Wang, Chang Xu, Huanan Wang, Changan Jiang
IF：
4.4070
Publish_to：
MOLECULAR IMMUNOLOGY
PMID：
33453658
Investigation on the Anaphylaxis and Anti-Digestive Stable Peptides Identification of Ultrasound-Treated α-Lactalbumin during In-Vitro Gastroduodenal Digestion
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Author：
Xumei Wang, Zongcai Tu, Guangxian Liu, Hui Wang, Yueming Hu, Tao Huang
IF：
4.3500
Publish_to：
Foods
PMID：
34829039
A fluorescent biosensor based on quantum dot–labeled streptavidin and poly-l-lysine for the rapid detection of Salmonella in milk
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Author：
Shuangyan Ding, Hailiang Hu, Xianglin Yue, Kaiwen Feng, Xiaoyu Gao, Qiuling Dong, Mingqi Yang, Ugur Tamer, Ganhui Huang, Jinsheng Zhang
IF：
4.2250
Publish_to：
JOURNAL OF DAIRY SCIENCE
PMID：
35181133
PLGA Nanoparticle-Based Dissolving Microneedle Vaccine of Clostridium perfringens ε Toxin
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Author：
Wei Wan, Yue Li, Jing Wang, Zhiying Jin, Wenwen Xin, Lin Kang, Junhong Wang, Xiaoyang Li, Yakun Cao, Hao Yang, Jinglin Wang, Shan Gao
IF：
4.2000
Publish_to：
Toxins
PMID：
37505730
rM2e-ΔPly protein immunization induces protection against influenza viruses and its co-infection with Streptococcus pneumoniae in mice
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Author：
Ting Guo, Jiangming Xiao, Lian Li, Wenlong Xu, Yuan Yuan, Yibing Yin, Xuemei Zhang
IF：
4.1740
Publish_to：
MOLECULAR IMMUNOLOGY
PMID：
36306645
Mutation in Transmembrane Domain 8 of Human Urate Transporter 1 Disrupts Uric Acid Recognition and Transport
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Author：
Qunsheng Lan, Ze’an Zhao, Hui Liao, Fengxin Zheng, Yongjun Chen, Ting Wu, Yuanxin Tian, Jianxin Pang
IF：
4.1320
Publish_to：
ACS Omega
PMID：
36188325
Trimerized S expressed by modified vaccinia virus Ankara (MVA) confers superior protection against lethal SARS-CoV-2 challenge in mice
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Author：
Junda Zhu, Zhenshan Wang, Yarui Li, Zihui Zhang, Shuning Ren, Jing Wang, Shijie Xie, Zhiyi Liao, Baifen Song, Wenxue Wu, Feihu Yan, Chen Peng
IF：
4.0000
Publish_to：
JOURNAL OF VIROLOGY
PMID：
38874361
Research on Detection of Ultra-Low Concentration Anthrax Protective Antigen Using Graphene Field-Effect Transistor Biosensor
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Author：
Ting Liang, Jingfei Chen, Rui Yan, Huaning Jiang, Hexi Li
IF：
3.9000
Publish_to：
SENSORS
PMID：
37447669
A Subunit Vaccine Harboring the Fusion Capsid Proteins of Porcine Circovirus Types 2, 3, and 4 Induces Protective Immune Responses in a Mouse Model
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Author：
Qikai Wang, Ran Zhang, Yue Wang, Ying Wang, Libin Liang, Haili Ma, Haidong Wang, Longlong Si, Xingchen Wu
IF：
3.8000
Publish_to：
Viruses-Basel
PMID：
39772270
Nanobodies as binding-chaperones stabilize the recombinant Bombyx mori acetylcholinesterase and protect the enzyme activity in pesticide detection
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Author：
Jun Cai, Ema Romão, Guangpei Wu, Jiadong Li, Liping Li, Zhifeng Wang, Yuwei Li, Jinyi Yang, Yudong Shen, Zhenlin Xu, Serge Muyldermans, Hong Wang
IF：
3.7050
Publish_to：
ENZYME AND MICROBIAL TECHNOLOGY
PMID：
35114480
Evaluation of the immunoprotective effects of porcine deltacoronavirus subunit vaccines
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Author：
Ruiming Yu, Liping Zhang, Peng Zhou, Zhongwang Zhang, Xiaoqing Liu, Yonglu Wang, Huichen Guo, Li Pan, Xinsheng Liu
IF：
3.7000
Publish_to：
VIROLOGY
PMID：
38070302
Screening of potential vaccine candidates against pathogenic Brucella spp. using compositive reverse vaccinology
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Author：
Zai Xiaodong, Yin Ying, Guo Fengyu, Yang Qiaoling, Li Ruihua, Li Yaohui, Zhang Jun, Xu Junjie, Chen Wei
IF：
3.6990
Publish_to：
VETERINARY RESEARCH
PMID：
34078437
Evidence of a shared binding site for Bacillus thuringiensis Cry1Ac and Cry2Aa toxins in Cnaphalocrocis medinalis cadherin
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Author：
J. Zhong, S. Fang, M. Gao, L. Lu, X. Zhang, Q. Zhu, Y. Liu, J. L. Jurat-Fuentes, X. Liu
IF：
3.5850
Publish_to：
INSECT MOLECULAR BIOLOGY
PMID：
34637177
Safety and protective efficacy of Salmonella Pullorum spiC and rfaH deletion rough mutant as a live attenuated DIVA vaccine candidate
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Author：
Xilong Kang, Yang Yang, Chuang Meng, Xinwei Wang, Bowen Liu, Shizhong Geng, Xinan Jiao, Zhiming Pan
IF：
3.3520
Publish_to：
POULTRY SCIENCE
PMID：
34991038
Activation of mTOR mediates hyperglycemia-induced renal glomerular endothelial hyperpermeability via the RhoA/ROCK/pMLC signaling pathway
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Author：
Chen Xiaolin, Chen Jianhui, Li Xianfan, Yu Zengpu
IF：
3.3200
Publish_to：
Diabetology & Metabolic Syndrome
PMID：
34627341
Construction and characterization of a gE/gI/TK-gene-deleted recombinant pseudorabies virus variant expressing the GP5 of the highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) and NADC30-like PRRSV
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Author：
Hui-Hua Zheng, Lin-Qing Wang, Cheng-Yao Hou, Ya-Peng Song, Shi Liu, Lan-Lan Zheng, Shi-Jie Ma, Hong-Ying Chen
IF：
3.3000
Publish_to：
MICROBIAL PATHOGENESIS
PMID：
40180235
USP7 Enables Immune Escape of Glioma Cells by Regulating PD-L1 Expression
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Author：
Bing Li, Bin Wang
IF：
3.0440
Publish_to：
IMMUNOLOGICAL INVESTIGATIONS
PMID：
35852892
Immune modulation of buffalo peripheral blood mononuclear cells by two asparaginyl endopeptidases from Fasciola gigantica
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Author：
Wu Dong-Qi, Guo Yan-Feng, Zou Yu, Tang Xiao-Ting, Zhang Wei-Yu, Di Wen-Da
IF：
3.0000
Publish_to：
Parasites & Vectors
PMID：
39696651
Enhanced immunogenicity of the tuberculosis subunit Rv0572c vaccine delivered in DMT liposome adjuvant as a BCG-booster
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Author：
Lirong Mao, Lifa Xu, Xiaochun Wang, Yingru Xing, Jian Wang, Yanpeng Zhang, Wei Yuan, Jianpeng Du, Zilun Shi, Jilei Ma, Jingyan Zhang, Xiaohan Zhang, Xinping Wang
IF：
2.9730
Publish_to：
TUBERCULOSIS
PMID：
35245739
LDL‑induced NLRC3 inflammasome activation in cardiac fibroblasts contributes to cardiomyocytic dysfunction
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Author：
Peng Wang, Wenbo Zhang, Zhen Feng, Jian Zhang, Ying Sun, Wei Zhang
IF：
2.9520
Publish_to：
Molecular Medicine Reports
PMID：
34036387
Identification of B-cell epitope on the N protein of Type 1 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) using Monoclonal Antibody and Construction of Epitope-mutated Virus
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Author：
Jiaqi Liu, Xindong Wang, Tongwei Ren, Jianguang Qin, Yifeng Qin, Kang Ouyang, Ying Chen, Weijian Huang, Zuzhang Wei
IF：
2.8000
Publish_to：
VIROLOGY
PMID：
38749084
The N- and C-terminal carbohydrate recognition domains of galectin-9 from Carassius auratus contribute differently to its immunity functions to Aeromonas hydrophila and Staphylococcus aureus
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Author：
Li Wang, Qiuxia Wang, Lei Wang, Shixiu Wu, Yan Yu, Yanhong Zhang, Pei Gao, Xianghui Kong, Jinyou Ma
IF：
2.7670
Publish_to：
JOURNAL OF FISH DISEASES
PMID：
34287946
Recombinant-attenuated Salmonella enterica serovar Choleraesuis vector expressing the PlpE protein of Pasteurella multocida protects mice from lethal challenge
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Author：
Zhou Guodong, Tian Jiashuo, Tian Yichen, Ma Qifeng, Li Quan, Wang Shifeng, Shi Huoying
IF：
2.6000
Publish_to：
BMC Veterinary Research
PMID：
37598169
Enhanced efficacy of dual chimeric antigen receptor-T cells targeting programmed death-ligand 1 and cancer-associated fibroblasts in colorectal cancer in vitro
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Author：
Yang Gao, CanJing Luo, Hua Yang, QiaoJin Xie, HaoJie He, JiaWei Li, JiDong Miao
IF：
2.5000
Publish_to：
CytoJournal
PMID：
40260068
Gallocatechin Gallate Inhibits the Replication of Pseudorabies Virus via Suppressing the Entry and Release Stages in Its Replication Cycle
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Author：
Zongyi Bo, Jinjin Zhu, Mengjiao Guo, Chengcheng Zhang, Yongzhong Cao, Xiaorong Zhang, Yantao Wu
IF：
2.4000
Publish_to：
Veterinary Sciences
PMID：
36977228
High-throughput sequencing of the porcine antibody repertoire with or without PEDV infection: A proof-of-concept study
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Author：
Chun-Miao Ji, Xiao-Feng Yang, Pan Qin, Bin Wang, Yao-Wei Huang
IF：
2.0140
Publish_to：
JOURNAL OF VIROLOGICAL METHODS
PMID：
33745967
Construction of constitutive expression of Eimeria tenella eukaryotic initiation factor U6L5H2 on the surface of Lactobacillus plantarum and evaluation of its immunoprotective efficiency against chicken coccidiosis
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Author：
Lingyu Sun, Yaru Lu, Ningning Zhao, Yakun Wang, Bingxiang Wang, Huihui Li, Zhiyuan Wu, Hongmei Li, Xiao Zhang, Xiaomin Zhao
IF：
1.8450
Publish_to：
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
PMID：
36272440
Screening, expression and anti-tumor functional identification of anti-LAG-3 nanobodies
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Author：
Dan Jiang, Rui Chen, Liyan Wang, Guangxian Xu
IF：
1.4000
Publish_to：
PROTEIN EXPRESSION AND PURIFICATION
PMID：
38851552
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