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英语
产品简介
| 英文名称 | EDTA decalcifying solution，pH 7，2 |
| 中文名称 | EDTA脱钙液（pH7.2） |
| 储存条件 | RT，1 year |
| 规格 | 100ml 500ml 1L |
| 单位 | 瓶 |
一些组织内含有骨质或钙化灶时，含钙的组织不宜直接用石蜡包埋切片。这是因为钙和石蜡之间的密度不同，较难切除完整的切片。对含钙组织最好固定之后，再进行脱钙或二者同时进行。然后进行下游的实验，如脱水、透明、浸蜡、包埋、切片。用于脱钙的试剂很多，脱钙剂包括有机酸、无机酸、乙二胺四乙酸（EDTA）以及电解法脱钙。
EDTA是一种相对较好的螯合脱钙剂，对组织结构影响最小，可以较好的保存组织的某些酶类， 经EDTA脱钙后的组织可以进行免疫组化和原位杂交染色。但是该法脱钙速度太慢，一般脱需要数周至数月。
Solarbio EDTA脱钙液优点：①经EDTA脱钙的组织染色结果好；②对组织的结构损害小；③用化学方法测试可以确定脱钙的终点。
Solarbio EDTA脱钙液缺点：①脱钙速度很慢，不适合常规标本脱钙使用；②脱钙后组织会稍微变硬。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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Occlusal trauma aggravates periodontitis through the plasminogen/plasmin system
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Author：
Xinran Liu, Jiaxin Li, Jinle Li, Tianqi Wang, Yan Ding, Yuan Yue, Min Wang, Na Wei, Liang Hao
IF：
2.9000
Publish_to：
ORAL DISEASES
PMID：
39039759
0.1% Nano-silver mediates PD-1/PD-L1 pathway and alleviates chronic apical periodontitis in rats
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Author：
Sun Xinxin, Li Boqi, Abula Dilinuer, Wang Liping, Wang Bangyao, Wang Qi, Liu Tao, Zhang Ruihan, Sheng Li, Jing Yinghao, Zhang Bei, Liu Yishan
IF：
2.8850
Publish_to：
Odontology
PMID：
36057921
Safety of Exposure to 0.2 T and 4 Hz Rotating Magnetic Field: A Ten-Month Study on C57BL/6 Mice
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Author：
Hua Yang, Yu Han, Cai Zhou, Shenglan Nie, Mengqing Li, Qinyao Yu, Yunpeng Wei, Xiaomei Wang
IF：
2.8000
Publish_to：
CURRENT ISSUES IN MOLECULAR BIOLOGY
PMID：
39057024
Granulocyte colony-stimulating factor (G-CSF) mediates bone resorption in periodontitis
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Author：
Yu Hui, Zhang Tianyi, Lu Haibin, Ma Qi, Zhao Dong, Sun Jiang, Wang Zuomin
IF：
2.7570
Publish_to：
BMC Oral Health
PMID：
34118920
Effect of Warm Acupuncture Combined with Bone Marrow Mesenchymal Stem Cells Transplantation on Cartilage Tissue in Rabbit Knee Osteoarthritis
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Author：
Jun-wei Liu, Yong-li Wu, Wei Wei, Yan-ling Zhang, Di Liu, Xiao-xiu Ma, Chun Li, Yu-yuan Ma
IF：
2.6300
Publish_to：
Evidence-based Complementary and Alternative Medicine
PMID：
34422071
Neobavaisoflavone improves medial collateral ligament-induced osteoarthritis through repressing the nuclear factor -κB/hypoxia-inducible factor-2α axis
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Author：
J Bai, T Liu, M Ren, X Wang
IF：
2.5890
Publish_to：
JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
PMID：
36942811
Histological Changes of Cervical Disc Tissue in Patients with Degenerative Ossification
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Author：
Yang Xiong, Ying-Li Yang, Yu-Shan Gao, Xiu-Mei Wang, Xing Yu
IF：
2.2490
Publish_to：
Journal of Korean Neurosurgical Society
PMID：
35108774
Low-level laser therapy alleviates periodontal age-related inflammation in diabetic mice via the GLUT1/mTOR pathway
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Author：
Cui Aimin, Sun Yuezhang, Zhu Kangjian, Zou Haonan, Yue Ziqi, Ding Yi, Song Xiuxiu, Chen Jiao, Ji Ning, Wang Qi
IF：
2.1000
Publish_to：
LASERS IN MEDICAL SCIENCE
PMID：
38236306
Survey anatomy and histological observation of the nasal cavity of Tupaia belangeri chinensis (Tupaiidae, Scandentia, Mammalia)
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Author：
Yiwei Feng, Wei Xia, Pengcheng Zhao, Xiang Yi, Anzhou Tang
IF：
2.0640
Publish_to：
Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology
PMID：
34605617
Anatomy and nomenclature of tree shrew lymphoid tissues
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Author：
Nan Shi, Wei Xia, Ketong Ji, Yiwei Feng, Hua Li, Guangyao He, Anzhou Tang
IF：
1.7980
Publish_to：
EXPERIMENTAL ANIMALS
PMID：
34853240
Experimental Design of Vertical Distraction Osteogenesis Using Simple 3 Screws
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Author：
Li Zeyu, Jiang Dongbo, Yao Yusheng
IF：
0.9000
Publish_to：
JOURNAL OF CRANIOFACIAL SURGERY
PMID：
36775875
技术问答
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注意：
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请问这一款脱钙液里面含有甲醛吗?2023-12-25 17:10:23
您好，是不含这一类固定剂成分的。
长期可以在37℃下进行脱钙吗?2023-12-11 13:44:50
您好，37℃条件下加速组织脱钙，如有必要可多次更换脱钙液，直至终点。
请问大鼠脱钙多久换一次液？一个50ml离心管里面装几根股骨合适?2023-10-15 15:42:15
您好，脱钙终点的判定方法：采用针刺、手掐、钳夹等方法，当骨组织变软或针刺时没有阻力感即可终止脱钙。脱钙液通常是使用样本 20～30 倍体积，如果想加速脱钙可以37℃进行脱钙以及增加换液频率。