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产品简介
| 别名 | 考马斯亮蓝染色液 |
| 英文名称 | Coomassie Brilliant Blue Fast Staining solution |
| 中文名称 | 考马斯亮蓝快速染色液 |
| 储存条件 | 2-8℃，1 year |
| 规格 | 500ml |
| 单位 | 瓶 |
货号：P1300
规格：500ml
组成：溶液A 10ml、溶液B 500ml。
保存：2-8℃保存，有效期1年。
产品简介：
考马斯亮蓝蛋白胶快速染色液是一种即用型快速灵敏而且安全的染液，以考马斯亮蓝G-250为染料，可用于SDS-PAGE或者非变性电泳蛋白凝胶的快速染色，染色时间短，半小时内即可检测到蛋白电泳条带。
使用方法： 工作液的配制，取100ml溶液B，加入2ml溶液A，混匀，此为工作液。此工作液配好后请在一周内使用完，过期效果会有一定的影响。
1. 将电泳后的 PAGE 胶（以8cm×10 cm大小为例）取下放入容器中，加入50 ml双蒸水或去离子水，加热至沸腾后停止，（可选：继续在脱色摇床上摇动5 分钟），弃去水溶液。
2. 加入25ml 快速染色工作液，加热至沸腾后保持沸腾状态30-60秒，停止加热后继续在脱色摇床上摇动 5-10 分钟，弃去染色液（此时蛋白条带应已可见）。
3. 加入约50 ml水，加热至沸腾后保持沸腾状态30-60秒，停止加热后继续在脱色摇床上摇动5-10分钟，换水即可完成脱色，观察结果。
注意事项：
1. 染色前的清洗步骤（第一步）中，水的质量非常重要，质量越好灵敏度越高，研究证明若用自来水加热清洗，染色效果与灵敏度仅与常规甲醇考马斯亮蓝染色（分子克隆第二版）相同。
2. 脱色时可用自来水清洗。
3. 若要得到无背景的染色胶，可重复脱色步骤或将胶放在水中过夜。
4. 经本产品染色的 PAGE 胶，胶的缩涨度小于 5%，染色后的胶可在水中放置数月而无明显脱色。
5. 每次加热后，继续在脱色摇床上摇动5分钟，可增强染色效果。
6. 本染色液有轻微腐蚀性，请带手套操作。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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技术问答
（1）
注意：
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。
京公网安备 11011202000391号
能不进行加热，直接染色吗?2022-10-12 13:02:46
您好，不能常温染色的，快染的是需要沸水浴处理的。