中文
英语
产品简介
| 英文名称 | Western Blocking Buffer |
| 中文名称 | 膜封闭液 |
| 储存条件 | 2-8℃，1 month |
| 规格 | 100ml 500ml |
| 单位 | 瓶 |
产品简介：
Western Blot 膜封闭液(Blocking Buffer)适用于 Western Blot 实验中 PVDF 膜或 NC 膜等转印膜的封闭。封 闭后，可以减小后续的一抗或二抗和膜的非特异性结合，降低背景，增强信噪比，以达到理想的显色效果。 按照每张膜封闭需要 5-10 毫升封闭液计算，一个包装的 Western 封闭液可以封闭 10-20 张膜。
使用方法：
本产品为即用型，无需稀释。蛋白转膜后将转印膜置于容器中，加入足够量的膜封闭液充分覆盖膜，室 温下振荡封闭半小时至 1 小时，即完成膜的封闭，然后进行一抗孵育等后续操作。
注意事项：
通常在室温封闭 60 分钟即可。对于一些背景非常高的抗体，可以 4℃封闭过夜。 为了您的安全和健康，请穿实验服并戴一次性手套操作。
相关产品：
A1010 30%丙烯酰胺 (29:1)
P1015 4×蛋白上样缓冲液（含 DTT）
PE0010 ECL Plus 荧光检测试剂(ECL 超敏发光液)
PR1800 Western blotting 蛋白 MARKER
SW3020 Western Blot 膜再生液
T1080 20×TBS
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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