Protein Description
The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. GFPSparkTM is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 508 nm) that is visible earlier than fluorescence of other green fluorescent proteins. GFPSparkTM is mainly intended for applications where fast appearance of bright fluorescence is crucial. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. It is specially recommended for cell and organelle abeling and tracking the promoter activity.
Gene Name Synonym:GFP
Protein Construction:A DNA sequence encoding the Aequorea victoria GFP (AAB65663) (Ser 2-Lys 238) was expressed, with a polyhistide tag at the N-terminus.
Source: Aequorea victoria
Expression Host: E. coli
QC Testing
Purity: >90 % as determined by SDS-PAGE
Bio Activity：Activity Assay
Endotoxin:Please contact us for more information.
Stability:Samples are stable for up to twelve months from date of receipt at -70 ℃
Predicted N terminal: Met
Molecular Mass:The recombinant Aequorea victoria GFP consisting of 253 amino acids and has a calculated molecular mass of 28.7 kDa. It migrates as an 35 kDa band in SDS-PAGE under reducing conditions as predicted.
Formulation:Lyophilized from sterile PBS, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.
Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage.
Avoid repeated freeze-thaw cycles.
Reconstitution:
Detailed reconstitution instructions are sent along with the products.