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产品简介
| 别名 | 植物蛋白抽提试剂盒 |
| 英文名称 | Plant Protein Extraction Kit |
| 中文名称 | 植物蛋白提取试剂盒 |
| 储存条件 | -20℃，avoid light，at least 1 year |
| 规格 | 50T 100T |
| 单位 | 盒 |
植物蛋白提取试剂盒用于植物组织中提取总蛋白，试剂盒中的裂解液含有蛋白酶抑制剂，作用温和，可快速获得总蛋白，可用于免疫印迹实验等基础研究实验，由于含有上述的酶抑制剂，不能用于研究蛋白激酶的研究。本品仅用于科研。
产品组成:
裂解液 100 ml 2-8℃
蛋白酶抑制剂（100x） 1 ml -20℃
PMSF（100x） 1ml
操作方法:
1、植物组织蛋白的提取
去100-200mg的植物组织放入液氮中（最好过夜），在液氮环境中将植物组织碾碎（越碎越好）；
加入1ml的裂解液，4℃裂解20min，期间每隔5min震荡1次；
4℃，14000转离心30min；
吸取上清至新的管中；
进行蛋白定量或者变性进行蛋白实验。
（提取好的蛋白建议保存于-80℃，即用即取，避免反复冻融，避免长时间储存。)
注意事项：
实验过程，所有试剂都要需要预冷或者即融，保证操作过程中的低温环境；
PSMF（有毒）现用现加，因为PMSF在水溶液中会快速降解；
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
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