中文
英语
产品简介
| 英文名称 | TMB Single-Component Substrate solution |
| 中文名称 | 单组分TMB显色液 |
| 储存条件 | 2-8℃，avoid light，3 years |
| 背景说明 | 高灵敏级 |
| 规格 | 100ml 500ml 1000ml |
| 单位 | 瓶 |
产品简介:
目前酶免疫分析（EIA)技术，已被广泛应用于抗原，半抗原或抗体的定量或定性检测分析。辣根过氧化物酶（HRP)及其偶联物是酶联免疫分析技术中常用的一种酶，由于3,3',5,5'-四甲基联苯胺（TMB)在HRP的显色反应体系中，比其它色原具有更高的灵敏度且无致癌性而被广泛应用。TMB主要应用于酶联免疫吸附实验（ELISA)，免疫斑点杂交或者免疫组化以及氯和过氧化氢的检测分析。为了满足不同的试剂盒产品研发与生产以及科研需求，本公司专门研制了针对不同类型的基于HRP的免疫分析用TMB显色液。
产品特点：
即用型：无需混合，方便快捷，减少误差
灵敏度高：不低于A、B液
稳定性好：2-8℃保存，有效期不低于36个月；显色终止后读数稳定
背景低：底物溶液在650nm检测时检测OD值小于0.04
质量可靠：产品批间差异小
外观：本试剂一般情况下为无色，有时略显浅蓝色或浅黄色
使用方法：
用适当的干净容器（用纯净水反复冲洗），倒出适量的单组分TMB显色液，待达到室温后即可使用。
加液：加完HRP结合物并孵育一定时间后，用适当洗涤液洗板3-5次，每孔加TMB显色液100ul。 根据个人实验需要，在室温（15-25℃）或37℃下避光温育10-30分钟或更长时间，直至显色至预期深浅。
终止：加入等体积的1M 盐酸或硫酸溶液终止反应，孔中反应液由蓝色变为黄色。
读数：终止反应后30分钟内在450nm处测定吸光值。
注意：如果出现高的反应背景或沉淀，表明TMB底物反应过于强烈。为了避免产生沉淀，可在终止后马上读数；或者进一步稀释一抗和/或HRP结合物。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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Hongbo Chen, Zhongping Xie, Runxiang Long, Shengtao Fan, Heng Li, Zhanlong He, Kangwei Xu, Yun Liao, Lichun Wang, Ying Zhang, Xueqi Li, Xingqi Dong, Tangwei Mou, Xiaofang Zhou, Yaoyun Yang, Lei Guo, Jianbo Yang, Huiwen Zheng, Xingli Xu, Jing Li, Yan Liang
IF：
6.6980
Publish_to：
Molecular Therapy-Methods & Clinical Development
PMID：
34462721
Smartphone recognition-based immune microparticles for rapid on-site visual data-sharing detection of Newcastle disease virus
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Author：
Shenjunjie Lu, Binglin Ma, Haoran Zhou, Yayang Li, Zhihao Qiao, Tong Xiao, Husheng Li, Baijie Wang, Meihui Cui, Shufang Zhang, Jin Chang, Taofeng Du, Jing Liu, Hanjie Wang
IF：
6.5560
Publish_to：
TALANTA
PMID：
35994803
GAP-43 targeted indocyanine green-loaded near-infrared fluorescent probe for real-time mapping of perineural invasion lesions in pancreatic cancer in vivo
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Author：
Wen Liang Lu, Houfang Kuang, Jianyou Gu, Xiaojun Hu, Bo Chen, Yingfang Fan
IF：
6.4580
Publish_to：
Nanomedicine-Nanotechnology Biology and Medicine
PMID：
37054805
Knob domain of Fiber 2 protein provides full protection against fowl adenovirus serotype 4
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Author：
Yapeng Song, Zhenchao Zhao, Lin Liu, Yifei Li, Wenming Gao, Xiaonan Song, Xinsheng Li
IF：
6.2860
Publish_to：
VIRUS RESEARCH
PMID：
37040821
Isolation and Characterization of Targeting-HBsAg VNAR Single Domain Antibodies from Whitespotted Bamboo Sharks (Chiloscyllium plagiosum)
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Author：
Xierui Jiang, Shan Sun, Zengpeng Li, Mingliang Chen
IF：
6.0850
Publish_to：
Marine Drugs
PMID：
37103376
Structural Basis of a Novel Agonistic Anti-OX40 Antibody
点击查看 >>
Author：
Jing Zhang, Xiaoyong Jiang, Han Gao, Fei Zhang, Xin Zhang, Aiwu Zhou, Ting Xu, Haiyan Cai
IF：
6.0640
Publish_to：
Biomolecules
PMID：
36139048
Generation of Human Domain Antibody Fragments as Potential Insecticidal Agents against Helicoverpa armigera by Cadherin-Based Screening
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Author：
Xiaodan Hu, Xiao Zhang, Yuan Liu, Meijing Gao, Manman Lin, Yajing Xie, Qing Zhu, Chongxin Xu, Xianjin Liu, Dalene Vosloo, Ofentse Jacob Pooe
IF：
5.8950
Publish_to：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
PMID：
35944165
Hypersensitivity in the lungs is responsible for acute respiratory failure in COVID-19 patients: Case series of patients who received high-dose/short-term methylprednisolone
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Author：
Meizhu Chen, Changli Tu, Cuiyan Tan, Xiaobin Zheng, Fengfei Sun, Yingjian Liang, Honglei Shi, Jian Wu, Yiying Huang, Zhenguo Wang, Kongqiu Wang, Minmin Lin, Weiming Wu, Hong Zhou, Jing Liu, Jin Huang
IF：
5.8710
Publish_to：
Clinical and Translational Allergy
PMID：
34484687
Delanzomib, a Novel Proteasome Inhibitor, Combined With Adalimumab Drastically Ameliorates Collagen-Induced Arthritis in Rats by Improving and Prolonging the Anti-TNF-α Effect of Adalimumab
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Author：
Wang Lei, Liu Lixiong, Hong Xiaoping, Liu Dongzhou, Cheng Zeneng
IF：
5.8110
Publish_to：
Frontiers in Pharmacology
PMID：
34880764
Characterization of a novel anti-PVRIG antibody with Fc-competent function that exerts strong antitumor effects via NK activation in preclinical models
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Author：
Xue Hongyu, Zhang Zhimin, Li Li, Zhu Chenjuan, Fei Keke, Sha Huijun, Wu Zhihai, Lin Xiaomin, Wang Feifei, Zhou Shuaixiang, Deng Xiya, Li Yiming, Chen Bingliang, Xiong Yao, Chen Kai
IF：
5.8000
Publish_to：
CANCER IMMUNOLOGY IMMUNOTHERAPY
PMID：
38554184
Co-expression With Replicating Vector Overcoming Competitive Effects Derived by a Companion Protease Inhibitor in Plants
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Author：
Ma Jiexue, Ding Xiangzhen, Li Zhiying, Wang Sheng
IF：
5.7540
Publish_to：
Frontiers in Plant Science
PMID：
34220920
Novel platform for engineering stable and effective vaccines against botulinum neurotoxins A, B and E
点击查看 >>
Author：
Liu Yang, Liu Xiaoyu, Chen Weiwei, Yu Yunzhou, Meng Jianghui, Wang Jiafu
IF：
5.7000
Publish_to：
Frontiers in Immunology
PMID：
39315101
Antiviral Effect of Ginsenosides rk1 against Influenza a Virus Infection by Targeting the Hemagglutinin 1-Mediated Virus Attachment
点击查看 >>
Author：
Xia Yang, Hailiang Sun, Zhening Zhang, Weixin Ou, Fengxiang Xu, Ling Luo, Yahong Liu, Weisan Chen, Jianxin Chen
IF：
5.6000
Publish_to：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
PMID：
36902398
Enzyme-Linked Aptamer Kits for Rapid, Visual, and Sensitive Determination of Lactoferrin in Dairy Products
点击查看 >>
Author：
Fan Zhang, Hongxia Du, Linsen Li, Tengfei Li, Jing Wang, Zilei Chen, Mengmeng Yan, Chao Zhu, Feng Qu
IF：
5.5610
Publish_to：
Foods
PMID：
36496570
Immunogenicity of rabies virus G mRNA formulated with lipid nanoparticles and nucleic acid immunostimulators in mice
点击查看 >>
Author：
Qiao Hongtu, Liu BoLi, Chen Jianguo, Peng Shusheng, Min Ming
IF：
5.5000
Publish_to：
VACCINE
PMID：
37866995
Screening,characterization and specific binding mechanism of aptamers against human plasminogen Kringle 5
点击查看 >>
Author：
Meijiao Duan, Kewei Li, Ling Zhang, Yaqi Zhou, Liujiao Bian, Cuiling Wang
IF：
5.3070
Publish_to：
BIOORGANIC CHEMISTRY
PMID：
37149949
Comparison of Chemiluminescence Enzyme Immunoassay (Cl-ELISA) with Colorimetric Enzyme Immunoassay (Co-ELISA) for Imidacloprid Detection in Vegetables
点击查看 >>
Author：
Rongqi Zhai, Ge Chen, Guangyang Liu, Xiaodong Huang, Xiaomin Xu, Lingyun Li, Yanguo Zhang, Donghui Xu, A. M. Abd El-Aty
IF：
5.2000
Publish_to：
Foods
PMID：
36613412
Construction, Characterization, and Application of a Nonpathogenic Virus-like Model for SARS-CoV-2 Nucleocapsid Protein by Phage Display
点击查看 >>
Author：
Yuting Wu, Bing Liu, Zhiwei Liu, Pengjie Zhang, Xihui Mu, Zhaoyang Tong
IF：
5.0750
Publish_to：
Toxins
PMID：
36287952
Identification and Characterization of a Novel Epitope of ASFV-Encoded dUTPase by Monoclonal Antibodies
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Author：
Shuai Zhang, Rui Wang, Xiaojing Zhu, Jiaxin Jin, Wenlong Lu, Xuyang Zhao, Bo Wan, Yifei Liao, Qin Zhao, Christopher L. Netherton, Guoqing Zhuang, Aijun Sun, Gaiping Zhang
IF：
5.0480
Publish_to：
Viruses-Basel
PMID：
34834981
Timolol induces necroptosis, apoptosis and senescence concentration-dependently in rabbit Limbal stem cells in vitro
点击查看 >>
Author：
Guo-Jian Jiang, Bei-Bei Li, Ting-Jun Fan
IF：
5.0370
Publish_to：
LIFE SCIENCES
PMID：
33798551
Oral Administration with Recombinant Attenuated Regulated Delayed Lysis Salmonella Vaccines Protecting against Staphylococcus aureus Kidney Abscess Formation
点击查看 >>
Author：
Yanchen Liang, Haochi Zhang, Na Pan, Yang Liu, Shouxin Sheng, Haotian Li, Xuemei Bao, Xiao Wang
IF：
4.9610
Publish_to：
Vaccines
PMID：
35891237
Identification of three novel B cell epitopes in ORF2 protein of the emerging goose astrovirus and their application
点击查看 >>
Author：
Ren Dan, Li Tuofan, Zhang Wei, Zhang Xinyun, Zhang Xinyu, Xie Quan, Zhang Jianjun, Shao Hongxia, Wan Zhimin, Qin Aijian, Ye Jianqiang, Gao Wei
IF：
4.8130
Publish_to：
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
PMID：
34921327
Fully synthetic phosphorylated Tau181, Tau217, and Tau231 calibrators for Alzheimer’s disease diagnosis
点击查看 >>
Author：
Xinyu Li, Huimei Zeng, Pradeepraj Durairaj, Weihuan Wen, Tianpeng Li, Yanru Zhao, Yang Liu, Xue Liu, Lingpeng Zhan, Lang Rao, Wen Yuan, Tengfei Guo, Weijun Shen, Hui Cai, Zhicheng Chen
IF：
4.8000
Publish_to：
Frontiers in Aging Neuroscience
PMID：
38288278
Evaluation of immunogenicity and protective efficacy of the outer membrane porin F (OprF) against Pseudomonas plecoglossicida in large yellow croaker (Larimichthys crocea)
点击查看 >>
Author：
Liangyin He, Jiale Kang, Xingfu Chen, Pan Qin, Xinhua Chen
IF：
4.7000
Publish_to：
FISH & SHELLFISH IMMUNOLOGY
PMID：
38316347
CgCaspase-3 activates the translocation of CgGSDME in haemocytes of Pacific oyster Crassostrea gigas
点击查看 >>
Author：
Xiaopeng Li, Xiaoxue Yan, Jinyuan Leng, Wei Wang, Yinan Li, Chuanyan Yang, Jiejie Sun, Lingling Wang, Linsheng Song
IF：
4.6220
Publish_to：
FISH & SHELLFISH IMMUNOLOGY
PMID：
36280129
A fast and ultrasensitive ELISA based on rolling circle amplification
点击查看 >>
Author：
Minli You, Ping Peng, Zhenrui Xue, Haoyang Tong, Wanghong He, Ping Mao, Qi Liu, Chunyan Yao, Feng Xu
IF：
4.6160
Publish_to：
ANALYST
PMID：
33899835
Albumin-binding DARPins as scaffold improve the hypoglycemic and anti-obesity effects of exendin-4 in vivo
点击查看 >>
Author：
Jinying Xia, Guosheng Gao, Changzhen Zhang, Jingjing Ying, Jianhui Li
IF：
4.6000
Publish_to：
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
PMID：
36906110
A fibrinogen-related protein mediates the recognition of various bacteria and haemocyte phagocytosis in oyster Crassostrea gigas
点击查看 >>
Author：
Wenwen Yang, Xiaoqian Lv, Jinyuan Leng, Yinan Li, Jiejie Sun, Chuanyan Yang, Lingling Wang, Linsheng Song
IF：
4.5810
Publish_to：
FISH & SHELLFISH IMMUNOLOGY
PMID：
33957267
Development of Broad-Spectrum Nanobodies for the Therapy and Diagnosis of SARS-CoV-2 and Its Multiple Variants
点击查看 >>
Author：
Lei He, Qian Wu, Zhaoyong Zhang, Lingling Chen, Kuai Yu, Leibin Li, Qiong Jia, Yanqun Wang, Jianqiang Ni, Chuanbin Wang, Qi Li, Xinyan Zhai, Jincun Zhao, Yuliang Liu, Ruiwen Fan, Yi-Ping Li
IF：
4.5000
Publish_to：
MOLECULAR PHARMACEUTICS
PMID：
38920116
Vip3Aa domain IV and V mutants confer higher insecticidal activity against Spodoptera frugiperda and Helicoverpa armigera
点击查看 >>
Author：
Xiaoxue Yang, Zeyu Wang, Lili Geng, Baoyan Chi, Rongmei Liu, Haitao Li, Jiguo Gao, Jie Zhang
IF：
4.4620
Publish_to：
PEST MANAGEMENT SCIENCE
PMID：
35243758
Alum Pickering Emulsion as Effective Adjuvant to Improve Malaria Vaccine Efficacy
点击查看 >>
Author：
Qiuting Chen, Nan Wu, Yuhui Gao, Xiaojun Wang, Jie Wu, Guanghui Ma
IF：
4.4220
Publish_to：
Vaccines
PMID：
34835175
A novel live attenuated duck Tembusu virus vaccine targeting N7 methyltransferase protects ducklings against pathogenic strains
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Author：
Wu Xuedong, Huang Shanzhi, Wang Mingshu, Chen Shun, Liu Mafeng, Zhu Dekang, Zhao Xinxin, Wu Ying, Yang Qiao, Zhang Shaqiu, Huang Juan, Ou Xumin, Zhang Ling, Liu Yunya, Yu Yanling, Gao Qun, Mao Sai, Sun Di, Tian Bin, Yin Zhongqiong, Jing Bo, Cheng Anchun,
IF：
4.4000
Publish_to：
VETERINARY RESEARCH
PMID：
37308988
Lipid A-modified Escherichia coli can produce porcine parvovirus virus-like particles with high immunogenicity and minimal endotoxin activity
点击查看 >>
Author：
Xuegang Shen, Yong-Bo Yang, Yanfei Gao, Shujie Wang, Haiwei Wang, Mingxia Sun, Fandan Meng, Yan-Dong Tang, Yabin Tu, Qingke Kong, Tong-Qing An, Xue-Hui Cai
IF：
4.3000
Publish_to：
Microbial Cell Factories
PMID：
39118114
Pplase of Dermatophagoides farinae promotes ovalbumin-induced airway allergy by modulating the functions of dendritic cells in a mouse model
点击查看 >>
Author：
Hui Wang, Lihua Mo, Xiaojun Xiao, Shu An, Xiaoyu L
IF：
4.2590
Publish_to：
Scientific Reports 7, Article number: 43322 (2017)
PMID：
28240301
A fluorescent biosensor based on quantum dot–labeled streptavidin and poly-l-lysine for the rapid detection of Salmonella in milk
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Author：
Shuangyan Ding, Hailiang Hu, Xianglin Yue, Kaiwen Feng, Xiaoyu Gao, Qiuling Dong, Mingqi Yang, Ugur Tamer, Ganhui Huang, Jinsheng Zhang
IF：
4.2250
Publish_to：
JOURNAL OF DAIRY SCIENCE
PMID：
35181133
Development of a novel ssDNA aptamer targeting cardiac troponin I and its clinical applications
点击查看 >>
Author：
Cen Yi, Wang Zhongping, Ke Peixiong, Zhu Wenting, Yuan Zhongwen, Feng Senling, Chen Yiqing, Lin Caiyan, Liu Xiaomin, Li Yuting, Yan Pengke
IF：
4.1570
Publish_to：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
PMID：
34673993
Mutation in Transmembrane Domain 8 of Human Urate Transporter 1 Disrupts Uric Acid Recognition and Transport
点击查看 >>
Author：
Qunsheng Lan, Ze’an Zhao, Hui Liao, Fengxin Zheng, Yongjun Chen, Ting Wu, Yuanxin Tian, Jianxin Pang
IF：
4.1320
Publish_to：
ACS Omega
PMID：
36188325
High hydrostatic pressure treatment reduces the potential antigenicity of β-conglycinin by changing the protein structure during in vitro digestion
点击查看 >>
Author：
Guanhao Bu, Tanghao Li
IF：
4.1250
Publish_to：
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
PMID：
34997598
Effect of transglutaminase crosslinking combined with lactic fermentation on the potential allergenicity and conformational structure of soy protein
点击查看 >>
Author：
Guangliang Xing, Siran Yang, Lu Huang, Sitong Liu, Xinyi Wan
IF：
4.1000
Publish_to：
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
PMID：
38817117
The SFT2D2 gene is associated with the autoimmune pathology of schizophrenia in a Chinese population
点击查看 >>
Author：
Liu Duilin, Wu Lin, Wei Hui, Zhu Caiyun, Tian Runhui, Zhu Wanwan, Xu Qi
IF：
4.0860
Publish_to：
Frontiers in Neurology
PMID：
36619926
Egg yolk immunoglobulin (IgY) targeting SARS‐CoV‐2 S1 as potential virus entry blocker
点击查看 >>
Author：
Bao Lirong, Zhang Cheng, Lyu Jinglu, Yi Ping, Shen Xin, Tang Boyu, Zhao Hang, Ren Biao, Kuang Yu, Zhou Linlin, Li Yan
IF：
4.0590
Publish_to：
JOURNAL OF APPLIED MICROBIOLOGY
PMID：
34706134
Identification of conserved linear epitopes in the SARS-CoV-2 receptor-binding region using monoclonal antibodies.
点击查看 >>
Author：
Yujie Yang, Liling Zhou, Chuncong Mo, Longbo Hu, Zhichao Zhou, Ye Fan, Wenkuan Liu, Xiao Li, Rong Zhou, Xingui Tian
IF：
4.0000
Publish_to：
Heliyon
PMID：
37292282
Research on Detection of Ultra-Low Concentration Anthrax Protective Antigen Using Graphene Field-Effect Transistor Biosensor
点击查看 >>
Author：
Ting Liang, Jingfei Chen, Rui Yan, Huaning Jiang, Hexi Li
IF：
3.9000
Publish_to：
SENSORS
PMID：
37447669
Elastin-like polypeptide-functionalized nanobody for column-free immunoaffinity purification of aflatoxin B1
点击查看 >>
Author：
Zhang Leping, Li Xiaojiang, He Qinghua, Chen Mengna, Zhou Mengmeng, Guo Jiebiao, Li Yanping, Tu Zhui
IF：
3.8000
Publish_to：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
PMID：
39264463
Porous NH2-MIL-101(Fe) metal organic framework for effective photocatalytic degradation of azo dye in wastewater treatment
点击查看 >>
Author：
Xuezhong Li, Yue Wang, Qi Guo
IF：
3.7760
Publish_to：
Heliyon
PMID：
35865975
Nanobodies as binding-chaperones stabilize the recombinant Bombyx mori acetylcholinesterase and protect the enzyme activity in pesticide detection
点击查看 >>
Author：
Jun Cai, Ema Romão, Guangpei Wu, Jiadong Li, Liping Li, Zhifeng Wang, Yuwei Li, Jinyi Yang, Yudong Shen, Zhenlin Xu, Serge Muyldermans, Hong Wang
IF：
3.7050
Publish_to：
ENZYME AND MICROBIAL TECHNOLOGY
PMID：
35114480
Evaluation of the immunoprotective effects of porcine deltacoronavirus subunit vaccines
点击查看 >>
Author：
Ruiming Yu, Liping Zhang, Peng Zhou, Zhongwang Zhang, Xiaoqing Liu, Yonglu Wang, Huichen Guo, Li Pan, Xinsheng Liu
IF：
3.7000
Publish_to：
VIROLOGY
PMID：
38070302
Nanobody Nb6 fused with porcine IgG Fc as the delivering tag to inhibit porcine reproductive and respiratory syndrome virus replication in porcine alveolar macrophages
点击查看 >>
Author：
Zhang Lu, Wang Lizhen, Cao Shuaishuai, Lv Huanhuan, Huang Jingjing, Zhang Guixi, Tabynov Kaissar, Zhao Qin, Zhou En-Min
IF：
3.6990
Publish_to：
VETERINARY RESEARCH
PMID：
33596995
A broad and potent IgM antibody against tetra-EV-As induced by EVA71 and CVA16 co-immunization
点击查看 >>
Author：
Wenbing Zhu, Zhuohang Liu, Xuelin Zheng, Jun Li, Kongjie Lu, Xi Jiang, Xuejian Zhang, Fangfang Ren, Xuemei Zhang, Jingwen Xu, Zhongxiang Wu, Ming Sun, Shaozhong Dong
IF：
3.6410
Publish_to：
VACCINE
PMID：
34600750
The effect of chlorophyll on the enzyme-linked immunosorbent assay (ELISA) of procymidone in vegetables and the way to overcome the matrix interference
点击查看 >>
Author：
Ziang Zhang, Hong Lin, Jianxin Sui, Xiangning Han, Luefeng Wang, Xun Sun, Limin Cao
IF：
3.6390
Publish_to：
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
PMID：
34825360
IgG antibody titers against SARS-CoV-2 nucleocapsid protein correlate with the severity of COVID-19 patients
点击查看 >>
Author：
Yang Li, Xu Qiang, Yang Bin, Li Jiayu, Dong Rong, Da Jingjing, Ye Zhixu, Xu Yongjie, Zhou Hourong, Zhang Xiangyan, Liu Lin, Zha Yan, Yu Fuxun
IF：
3.6050
Publish_to：
BMC MICROBIOLOGY
PMID：
34922455
Evidence of a shared binding site for Bacillus thuringiensis Cry1Ac and Cry2Aa toxins in Cnaphalocrocis medinalis cadherin
点击查看 >>
Author：
J. Zhong, S. Fang, M. Gao, L. Lu, X. Zhang, Q. Zhu, Y. Liu, J. L. Jurat-Fuentes, X. Liu
IF：
3.5850
Publish_to：
INSECT MOLECULAR BIOLOGY
PMID：
34637177
The Recombinant Expression Proteins FnBP and ClfA From Staphylococcus aureus in Addition to GapC and Sip From Streptococcus agalactiae Can Protect BALB/c Mice From Bacterial Infection
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Author：
Ma Zhongchen, Yin Xinyue, Wu Peng, Hu Ruirui, Wang Yong, Yi Jihai, Wang Zhen, Chen Chuangfu
IF：
3.4120
Publish_to：
Frontiers in Veterinary Science
PMID：
34250059
Safety and protective efficacy of Salmonella Pullorum spiC and rfaH deletion rough mutant as a live attenuated DIVA vaccine candidate
点击查看 >>
Author：
Xilong Kang, Yang Yang, Chuang Meng, Xinwei Wang, Bowen Liu, Shizhong Geng, Xinan Jiao, Zhiming Pan
IF：
3.3520
Publish_to：
POULTRY SCIENCE
PMID：
34991038
Activation of mTOR mediates hyperglycemia-induced renal glomerular endothelial hyperpermeability via the RhoA/ROCK/pMLC signaling pathway
点击查看 >>
Author：
Chen Xiaolin, Chen Jianhui, Li Xianfan, Yu Zengpu
IF：
3.3200
Publish_to：
Diabetology & Metabolic Syndrome
PMID：
34627341
Study on the mechanism of miRNAs on liver injury in the condition of Protoscocephalus alveolarus transhepatic portal vein infection
点击查看 >>
Author：
Yazhou Zhu, Ming Li, Zihua Li, Jiahui Song, Wei Zhao
IF：
3.2000
Publish_to：
Immunity Inflammation and Disease
PMID：
38652009
Infant rhesus macaques as a non-human primate model of Bordetella pertussis infection
点击查看 >>
Author：
Jiang Wenwen, Wei Chen, Mou Dachao, Zuo Weilun, Liang Jiangli, Ma Xiao, Wang Lichan, Gao Na, Gu Qin, Luo Peng, Ma Yan, Li Jingyan, Liu Shuyuan, Shi Li, Sun Mingbo
IF：
3.0900
Publish_to：
BMC INFECTIOUS DISEASES
PMID：
33941094
USP7 Enables Immune Escape of Glioma Cells by Regulating PD-L1 Expression
点击查看 >>
Author：
Bing Li, Bin Wang
IF：
3.0440
Publish_to：
IMMUNOLOGICAL INVESTIGATIONS
PMID：
35852892
Immune modulation of buffalo peripheral blood mononuclear cells by two asparaginyl endopeptidases from Fasciola gigantica
点击查看 >>
Author：
Wu Dong-Qi, Guo Yan-Feng, Zou Yu, Tang Xiao-Ting, Zhang Wei-Yu, Di Wen-Da
IF：
3.0000
Publish_to：
Parasites & Vectors
PMID：
39696651
Enhanced immunogenicity of the tuberculosis subunit Rv0572c vaccine delivered in DMT liposome adjuvant as a BCG-booster
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Author：
Lirong Mao, Lifa Xu, Xiaochun Wang, Yingru Xing, Jian Wang, Yanpeng Zhang, Wei Yuan, Jianpeng Du, Zilun Shi, Jilei Ma, Jingyan Zhang, Xiaohan Zhang, Xinping Wang
IF：
2.9730
Publish_to：
TUBERCULOSIS
PMID：
35245739
An immunoglobulin superfamily member (CgIgIT2) functions as immune inhibitory receptor to inhibit the inflammatory cytokine expressions in Crassostrea gigas
点击查看 >>
Author：
Xiaoxue Yan, Jiejie Sun, Wenwen Yang, Xiaopeng Li, Qian Yang, Yinan Li, Wei Wu, Ping Wei, Lingling Wang, Linsheng Song
IF：
2.9000
Publish_to：
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
PMID：
37044269
Identification of B-cell epitope on the N protein of Type 1 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) using Monoclonal Antibody and Construction of Epitope-mutated Virus
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Author：
Jiaqi Liu, Xindong Wang, Tongwei Ren, Jianguang Qin, Yifeng Qin, Kang Ouyang, Ying Chen, Weijian Huang, Zuzhang Wei
IF：
2.8000
Publish_to：
VIROLOGY
PMID：
38749084
Development of an indirect ELISA to detect PEDV specific IgA antibody based on a PEDV epidemic strain
点击查看 >>
Author：
Wang Kun, Hu Zhiqiang, Fan Mingyu, Shao Zhenwen, Yu Qiannan, Li Xiaowen
IF：
2.7920
Publish_to：
BMC Veterinary Research
PMID：
35982455
The N- and C-terminal carbohydrate recognition domains of galectin-9 from Carassius auratus contribute differently to its immunity functions to Aeromonas hydrophila and Staphylococcus aureus
点击查看 >>
Author：
Li Wang, Qiuxia Wang, Lei Wang, Shixiu Wu, Yan Yu, Yanhong Zhang, Pei Gao, Xianghui Kong, Jinyou Ma
IF：
2.7670
Publish_to：
JOURNAL OF FISH DISEASES
PMID：
34287946
Identification of a target polypeptide of the CD169 receptor of bovine macrophage using a phage display peptide library
点击查看 >>
Author：
Chunyu Tong, Qi Liu, Zhelin Zhang, Yimin Liang, Wenzhi Feng, Honghao Yu, Di Lan, Qianyi Liu, Bocui Song
IF：
2.7000
Publish_to：
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
PMID：
40086709
A peptide-based ELISA for detection of antibodies against novel goose astrovirus type 1
点击查看 >>
Author：
Dan Ren, Xinyun Zhang, Wei Zhang, Mingjun Lian, Xianchen Meng, Tuofan Li, Quan Xie, Hongxia Shao, Zhimin Wan, Aijian Qin, Wei Gao, Jianqiang Ye
IF：
2.6230
Publish_to：
JOURNAL OF VIROLOGICAL METHODS
PMID：
36356679
Antibody preparation and age-dependent distribution of TLR8 in Bactrian camel spleens
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Author：
Fang Ying-Dong, Liu Jing-Yu, Xie Fei, Liu Li-Ping, Zeng Wei-Wei, Wang Wen-Hui
IF：
2.6000
Publish_to：
BMC Veterinary Research
PMID：
38104080
Effect of active immunization with OPN5 on follicular development and egg production in quail under different photoperiods
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Author：
Xiaoli Zhou, Danli Jiang, Zhuoshen Zhang, Xu Shen, Jianqiu Pan, Hongjia Ouyang, Danning Xu, Yunbo Tian, Yunmao Huang
IF：
2.4000
Publish_to：
THERIOGENOLOGY
PMID：
39116655
Development of a monoclonal antibody-based competitive enzyme-linked immunosorbent assay for detection of antibodies against duck adenovirus 3
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Author：
Yun Lin, Yiwen Niu, Wenyuan Zhang, Weikang Wang, Quan Xie, Tuofan Li, Shengnan Wang, Hongxia Shao, Zhimin Wan, Aijian Qin, Jianqiang Ye
IF：
2.3000
Publish_to：
BMC Veterinary Research
PMID：
40156007
High-throughput sequencing of the porcine antibody repertoire with or without PEDV infection: A proof-of-concept study
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Author：
Chun-Miao Ji, Xiao-Feng Yang, Pan Qin, Bin Wang, Yao-Wei Huang
IF：
2.0140
Publish_to：
JOURNAL OF VIROLOGICAL METHODS
PMID：
33745967
Construction of constitutive expression of Eimeria tenella eukaryotic initiation factor U6L5H2 on the surface of Lactobacillus plantarum and evaluation of its immunoprotective efficiency against chicken coccidiosis
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Author：
Lingyu Sun, Yaru Lu, Ningning Zhao, Yakun Wang, Bingxiang Wang, Huihui Li, Zhiyuan Wu, Hongmei Li, Xiao Zhang, Xiaomin Zhao
IF：
1.8450
Publish_to：
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
PMID：
36272440
Protective efficacy of a novel multivalent vaccine in the prevention of diarrhea induced by enterotoxigenic Escherichia coli in a murine model
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Author：
Hong Zhao, Yongping Xu, Gen Li, Xin Liu, Xiaoyu Li, Lili Wang
IF：
1.6720
Publish_to：
Journal of Veterinary Science
PMID：
34841745
Screening, expression and anti-tumor functional identification of anti-LAG-3 nanobodies
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Author：
Dan Jiang, Rui Chen, Liyan Wang, Guangxian Xu
IF：
1.4000
Publish_to：
PROTEIN EXPRESSION AND PURIFICATION
PMID：
38851552
Protocol for evaluating humoral immune responses in mice following SARS-CoV-2 vaccination
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Author：
Yao Zhang, Shixiong Li, Jingyou Yu
IF：
1.3000
Publish_to：
STAR Protocols
PMID：
39823234
An efficient peptide-based ELISA for differentiating fowl adenovirus 4–infected chickens from vaccinated chickens
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Author：
Songhua Xie, Qiuping Shen, Wei Zhang, Weikang Wang, Quan Xie, Tuofan Li, Zhimin Wan, Hongxia Shao, Aijian Qin, Jianqiang Ye
IF：
1.2790
Publish_to：
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
PMID：
33856244
技术问答
（3）
注意：
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
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单组分与双组份是什么区别？做Elisa应该用哪个?2023-04-13 21:29:25
您好，组成成分不同，操作步骤有所区别，实验效果差别不大两个都可以选择。
ph是多少呀?2023-04-13 20:08:11
您好，ph为3.95-4。
终止液可以用磷酸吗？多少浓度?2023-04-04 10:59:58
您好，具体浓度需要查阅相关资料确定。