中文
英语
产品简介
| 英文名称 | Lowry Protein Assay Kit |
| 中文名称 | Lowry法蛋白浓度测定试剂盒-1000微孔 |
| 储存条件 | BSA附件-20℃，其它试剂RT，有效期1年 |
| 规格 | 100T(1000微孔) |
| 单位 | 盒 |
Lowry法包括两步反应：第一步是在碱性条件下，蛋白质与铜作用生成蛋白质-铜络合物；第二步是此络合物将Folin试剂还原，产生深蓝色，颜色深浅与蛋白质含量成正比。定量范围为5～500μg/ml蛋白质。Folin试剂显色反应由酪氨酸、色氨酸和半胱氨酸引起，因此样品中若含有酚类、柠檬酸和巯基化合物均有干扰作用。此外，不同蛋白质因酪氨酸、色氨酸含量不同而使显色强度稍有不同。
Lowry法测定较为不受脂类物质干扰，适于脂类含量较高的样品测定。也能耐受相当浓度的去垢剂如SDS。
组成与储存：
组分名称 规格 保存
Folin酚甲试剂A 100ml×2 2-8℃
Folin酚甲试剂B 5ml 2-8℃
Folin酚乙试剂(1N) 20ml 2-8℃
BSA标准液5 mg/ml 1ml -20ºC冻存
可进行1000T微板(microplate)测定或100T 2ml比色杯测定。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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IF：
2.5000
Publish_to：
MOLECULAR REPRODUCTION AND DEVELOPMENT
PMID：
38018308
DJ‑1 affects oxidative stress and pyroptosis in hippocampal neurons of Alzheimer's disease mouse model by regulating the Nrf2 pathway
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Author：
Lin Cheng, Wei Zhang
IF：
2.4470
Publish_to：
Experimental and Therapeutic Medicine
PMID：
33850529
Dimethyl fumarate prevents acute lung injury related cognitive impairment potentially via reducing inflammation
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Author：
Wang Xiaowei, Wang Yanbo, Pan Haiyan, Yan Ci
IF：
1.6370
Publish_to：
Journal of Cardiothoracic Surgery
PMID：
34772431
Microglia increase CEMIP expression and promote brain metastasis in breast cancer through the JAK2/STAT3 signaling pathway
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Author：
Qi Qin, Chaoying Wang, Yongfu Li, Qiuyu Mo
IF：
0.9000
Publish_to：
Oncologie
PMID：
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