中文
英语
产品简介
| 英文名称 | EDTA Antigen Retrieval Solution，50× |
| 中文名称 | EDTA抗原修复液（50X） |
| 储存条件 | RT，1 year |
| 规格 | 100ml 500ml |
| 单位 | 瓶 |
细胞或组织用多聚甲醛、甲醛或其它醛类试剂固定后，会导致细胞内抗原形成醛键、羧甲键而被封闭了部分抗原决定簇，同时蛋白之间发生交联而使抗原决定簇隐蔽，导致免疫染色时染色信号减弱，甚至出现一些假阴性染色结果。所以要求在进行免疫组化染色时，需要先进行抗原修复或暴露，即将固定时分子之间所形成的交联破坏，而恢复抗原的原有空间形态。从而提高抗原的检出率，降低背景染色，提高检测的准确性。
EDTA抗原修复液(EDTA Antigen Retrieval solution，50×)是一种常用的抗原修复液，可以用于石蜡切片、冰冻切片等样品使用多聚甲醛、甲醛或其它醛类试剂固定后的抗原修复。可以有效去除醛类固定试剂导致的蛋白之间的交联，充分暴露石蜡切片等样品中的抗原表位，从而大大改善免疫染色效果。
通常石蜡切片都需进行抗原修复处理，而冰冻切片可以不进行抗原修复处理。抗原修复可以提高石蜡切片的免疫染色效果，亦可以不同程度的提高冰冻切片的染色效果。当冰冻切片免疫染色效果不理想时，考虑进行抗原修复。
按照每个片子需要10ml抗原修复液(1×)计算，100ml抗原修复液(50×)可以用于500个样本的抗原修复。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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技术问答
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ph值为多少?2023-08-14 13:39:46
您好，PH值为8.0。
EDTA抗原修复液的使用终浓度是多少?2023-05-28 11:07:29
您好，本产品是50×的浓缩液，使用之前按需要量用去离子水或双蒸水稀释至1×。
稀释到1x后PH是多少?2022-12-07 09:51:56
您好，PH在8.0左右。