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产品简介
| 英文名称 | Native lysis Buffer |
| 中文名称 | 非变性组织/细胞裂解液 |
| 储存条件 | 附件PMSF-20℃，裂解液2-8℃。有效期1年 |
| 规格 | 100ml 500ml |
| 单位 | 瓶 |
非变性组织/细胞裂解缓冲液内含非离子型去垢剂，能裂解细胞并在非变性条件下释放胞浆蛋白和可溶性膜蛋白、核蛋白。非变性条件下的裂解蛋白产物最大限度地保留了蛋白的特性和功能，如抗原-抗体结合或酶学活性，因此适宜于进行免疫共沉淀。另外，有些抗体对非变性蛋白具有更高的结合能力或只能识别非变性蛋白的抗原位点，这种情况应该使用非变性裂解。
使用说明：
根据使用量，取每 1ml 裂解液加入 10ul PMSF，使 PMSF 的最终浓度为 1mM。混匀备用（PMSF 现用现加）。
1、样品前处理：
a)对于贴壁细胞：去除培养液，用 PBS、生理盐水或无血清培养液洗一遍。按照 6 孔板每孔加入 150-250ul裂解液的比例加入裂解液。用枪吹打数下，使裂解液和细胞充分接触，冰上放置裂解 5-10 分钟。
b)对于悬浮细胞：离心收集细胞，用手指把细胞用力弹散。按照 6 孔板每孔细胞加入 150-250 微升裂解液的比例加入裂解液，冰上放置裂解 5-10 分钟。期间可以用手指轻弹以充分裂解细胞。充分裂解后应没有明显的细胞沉淀。如果细胞量较多，必需分装成 50-100 万细胞/管，然后再裂解。
c)对于组织样品：把组织剪切成细小的碎片。按照每 20 毫克组织加入 150-250 微升裂解液的比例加入裂解液。(如果裂解不充分可以适当添加更多的裂解液，如果需要高浓度的蛋白样品，可以适当减少裂解液的用量) 。 用玻璃匀浆器匀浆，直至充分裂解。
2、后处理：
充分裂解后，将裂解后的样品 10000-14000g 离心3-5 分钟，取上清，即可进行后续的 PAGE、Western 和免疫沉淀、免疫共沉淀等操作。
注意事项：
为取得最佳的使用效果，尽量避免过多的反复冻融。可以适当分装后使用。裂解样品的所有步骤都需在冰上或 4℃进行。裂解时间可根据实验要求进行优化处理。
非变性蛋白裂解液裂解得到的蛋白样品，可以用 BCA蛋白浓度测定试剂盒测定蛋白浓度。由于含有较高浓度的 Triton X-100 等干扰物质，不能用 Bradford 法测定由本裂解液裂解得到样品的蛋白浓度。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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Wei Feng, Xiao-Qin Zhong, Xue-Xia Zheng, Qing-Ping Liu, Min-Ying Liu, Xiao-Bao Liu, Chang-Song Lin, Qiang Xu
IF：
4.4930
Publish_to：
Journal of Immunology Research
PMID：
37252680
Multiomics analyses reveals Anaplasma phagocytophilum Ats-1 induces anti-apoptosis and energy metabolism by upregulating the respiratory chain-mPTP axis in eukaryotic mitochondria
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Author：
Li Ruirui, Ma Zhongchen, Zheng Wei, Wang Zhen, Yi Jihai, Xiao Yangyang, Wang Yong, Chen Chuangfu
IF：
4.4650
Publish_to：
BMC MICROBIOLOGY
PMID：
36357826
Investigating the Impact of Pigmentation Variation of Breast Muscle on Growth Traits, Melanin Deposition, and Gene Expression in Xuefeng black-bone Chickens
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Author：
Yuying Deng, Xiangyong Qu, Yaling Yao, Meichun Li, Changqing He, Songchang Guo
IF：
4.4000
Publish_to：
POULTRY SCIENCE
PMID：
38598910
Nucleoporin 93, a new substrate of the E3 ubiquitin protein ligase HECTD1, promotes esophageal squamous cell carcinoma progression
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Author：
Zhang Jinfeng, Xin Yanzhong, Ling Xiaodong, Liang Hao, Zhang Luquan, Fang Chengyuan, Ma Jianqun
IF：
4.3000
Publish_to：
Human Cell
PMID：
37993750
Ultrasound-assisted free radical modification on the structural and functional properties of ovalbumin-epigallocatechin gallate (EGCG) conjugates
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Author：
Zihao Li, Senlin Ji, Chuyu Wu, Jiayong Wu, Runjing Cao, Yunshu Wang, Yun Xu, Jingwei Li, Cun-Jin Zhang
IF：
4.2000
Publish_to：
NEUROCHEMISTRY INTERNATIONAL
PMID：
37385448
FOXA2 inhibits doxorubicin-induced apoptosis via transcriptionally activating HBP rate-limiting enzyme GFPT1 in HCC cells
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Author：
Huang Huang, Wang Yuhan, Huang Tianmiao, Wang Lingyan, Liu Yangzhi, Wu Qiong, Yu Ang, Shi Meiyun, Wang Xiaoyu, Li Wenli, Zhang Jianing, Liu Yubo
IF：
4.1580
Publish_to：
JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY
PMID：
34291417
Molecular Evolution and Functional Characterization of PKC-α-like in Lamprey
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Author：
Zhulin Liu, Zhanfeng Gao, Jiali Lu, Xingzhu Zhang, Kaixia Ren, Xue Li, Feng Sun, Huan Zhao, Qingwei Li, Yang Xu, Meng Gou, Yinglun Han
IF：
4.1000
Publish_to：
FISH & SHELLFISH IMMUNOLOGY
PMID：
40015494
Function and mechanism of exogenous AGR2 in colorectal cancer cells
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Author：
Chao Zheng, Yu Mao, Jianping Ye, Miaolong Zhang, Yongfeng Chen
IF：
4.0000
Publish_to：
Heliyon
PMID：
38560175
The role of protein arginine deiminase 4-dependent neutrophil extracellular traps formation in ulcerative colitis
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Author：
Xiaogang Song, Chao Wei, Hui Huang, Xingdan Cao, Ziyi Chen, Yongqing Chen, Bing Wu
IF：
3.9000
Publish_to：
Food Science & Nutrition
PMID：
Bazi Bushen capsule attenuates cognitive deficits by inhibiting microglia activation and cellular senescence
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Author：
Chuanyuan Ji, Cong Wei, Mengnan Li, Shuang Shen, Shixiong Zhang, Yunlong Hou, Yiling Wu
IF：
3.8890
Publish_to：
PHARMACEUTICAL BIOLOGY
PMID：
36263579
ALKBH5 inhibitors as a potential treatment strategy in heart failure—inferences from gene expression profiling
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Author：
Sumra Komal, Atia Gohar, Saad Althobaiti, Ishtiaq Ahmad Khan, Liu-Gen Cui, Li-Rong Zhang, Sheng-Na Han, Muhammad Shakeel
IF：
3.6000
Publish_to：
Frontiers in Cardiovascular Medicine
PMID：
37583580
Activating autophagy and ferroptosis of 3‑Chloropropane‑1,2‑diol induces injury of human umbilical vein endothelial cells via AMPK/mTOR/ULK1
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Author：
Xin Yi, Xiao Long, Canzhang Liu
IF：
3.4000
Publish_to：
Molecular Medicine Reports
PMID：
36799162
L-Arginine Enhances Protein Synthesis by Phosphorylating mTOR (Thr 2446) in a Nitric Oxide-Dependent Manner in C2C12 Cells
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Author：
R Wang, H Jiao, J Zhao, X Wang, H Lin
IF：
3.3930
Publish_to：
Oxidative Medicine and Cellular Longevity
PMID：
29854093
Resveratrol promotes oxidative stress to drive DLC1 mediated cellular senescence in cancer cells
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Author：
S Ji, Z Zheng, S Liu, G Ren, J Gao, Y Zhang
IF：
3.3090
Publish_to：
Experimental cell research
PMID：
29964052
CD4 is an important host factor for Japanese encephalitis virus entry and replication in PK-15 cells
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Author：
Qi Wang, Shuqing Yang, Ke Yang, Xinran Li, Yu Dai, Yi Zheng, Sanjie Cao, Qigui Yan, Xiaobo Huang, Yiping Wen, Qin Zhao, Senyan Du, Yifei Lang, Shan Zhao, Rui Wu
IF：
3.3000
Publish_to：
VETERINARY MICROBIOLOGY
PMID：
38006719
Protein kinase C inhibitor chelerythrine attenuates partial unilateral ureteral obstruction induced kidney injury in neonatal rats
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Author：
Bo Shi,Shixing Li,Hao Ju,Xin Liu,Dan Li,Ying Li
IF：
3.2340
Publish_to：
Life Sciences
PMID：
30439378
lncRNA SNHG6 Knockdown Promotes Microglial M2 Polarization and Alleviates Spinal Cord Injury via Regulating the miR-182-5p/NEUROD4 Axis
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Author：
Feng Luqian, Li Gang
IF：
3.1000
Publish_to：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
PMID：
39832102
Exploring the mechanism of Astragali radix for promoting osteogenic differentiation based on network pharmacology, molecular docking, and experimental validation
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Author：
Zenghui Tian, Yingying Li, Xiaoying Wang, Kaiying Cui, Jinxing Guo, Mingliang Wang, Yanke Hao, Farong Zhang
IF：
3.0000
Publish_to：
Chemical Biology & Drug Design
PMID：
37690812
YY1-induced LncRNA-TUG1 elevates YOD1 to promote cell proliferation and inhibit bortezomib sensitivity in multiple myeloma
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Author：
Qingqing Yin, Xianjuan Shen, Honglei Xu, Wei Feng, Xiuying Shi, Shaoqing Ju
IF：
2.9960
Publish_to：
LEUKEMIA & LYMPHOMA
PMID：
37078241
Detection of LPS-induced macrophage activation with single-cell resolution through DC insulator-based electrokinetic devices
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Author：
Yameng Liu
IF：
2.9000
Publish_to：
ELECTROPHORESIS
PMID：
36996424
Uncarboxylated osteocalcin promotes proliferation and metastasis of MDA-MB-231 cells through TGF-β/SMAD3 signaling pathway
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Author：
Xu Jiaojiao, Ma Luyao, Wang Danqing, Yang Jianhong
IF：
2.8130
Publish_to：
BMC Molecular and Cell Biology
PMID：
35413833
LncRNA MSTO2P affects the proliferation, invasion and migration of non-small cell lung cancer by regulating the Wnt/β-catenin pathway
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Author：
Chen Yunfeng, Shi Hai, Dong Yuan, Cui Wenjie
IF：
2.8000
Publish_to：
Discover Oncology
PMID：
39928213
Antigen retrieval pre-treatment causes a different expression pattern of Cav3.2 in rat and mouse spinal dorsal horn
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Author：
Xiao E Cheng, Long Xian Ma, Xiao Jin Feng,Meng Ye
IF：
2.7020
Publish_to：
Life sciences
PMID：
30678436
Rhythmic deep-brain magnetic stimulation promotes angiogenesis and inhibits apoptosis by regulating the JAK2/STAT3 pathway in rats with superior sagittal sinus thrombosis
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Author：
Min Li, Jiahao Chen, Ran Meng, Xunming Ji
IF：
2.7000
Publish_to：
BRAIN RESEARCH
PMID：
39956380
The intervention mechanism of Tanshinone IIA in alleviating neuronal injury induced by HMGB1 or TNF-α-mediated microglial activation
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Author：
Yan-Zhu Quan, Jing-He Wang, Si-Hui Zhang, Guang-Nan Jin, Jing-Mei Lu, Yi-Ming Liu, Hong-Yan Gao, Jin-Yi Zhou, Bing-Zhe Wang, Yan Xin, Yue-Xian Cui, Xiang Xu, Lian-Xun Piao
IF：
2.6000
Publish_to：
TOXICOLOGY IN VITRO
PMID：
39357688
Interfering with UBE2L3 expression targets regulation of MLKL to promote necroptosis inhibition of growth in osteosarcoma
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Author：
Zhao Xiwu, Shan Guoqiang, Xing Deguo, Gao Hongwei, Xiong Zhenggang, Hui Wenpeng, Gong Mingzhi
IF：
2.5000
Publish_to：
World Journal of Surgical Oncology
PMID：
39988669
DR6 Augments Colorectal Cancer Cell Growth, Invasion, and Stemness by Activating AKT/NF-κB Pathway
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Author：
Jia Jing, Huang Yisen, Chen Qiwei, Hou Jianbin, Liu Yan, Xie Lifeng, Li Xinyu, Yang Chunkang
IF：
2.4000
Publish_to：
BIOCHEMICAL GENETICS
PMID：
38478147
Creatine kinase mitochondrial 2 promotes the growth and progression of colorectal cancer via enhancing Warburg effect through lactate dehydrogenase B
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Author：
Shasha Cai, Qingqing Xia, Darong Duan, Junhui Fu, Zhenxing Wu, Zaixing Yang, Changfa Yu
IF：
2.3000
Publish_to：
PeerJ
PMID：
38952967
Silencing of long noncoding RNA MIAT inhibits the viability and proliferation of breast cancer cells by promoting miR-378a-5p expression
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Author：
Chao Yan, Yue Jin
IF：
2.1230
Publish_to：
Open Medicine
PMID：
37025425
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Author：
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IF：
1.8690
Publish_to：
Home /Anti-Cancer Drugs
PMID：
26799652
Environmental enrichment attenuates sevoflurane anesthesia-induced learning deficits in aged mice through regulating TTBK1 and phosphorylated Tau expression
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Author：
Chang Xuenan, Zhuang Xiaoli, Feng Jingyu, Yu Yonghao, Yu Yang
IF：
1.7000
Publish_to：
EXPERIMENTAL BRAIN RESEARCH
PMID：
39953251
TREM-1 expression in rat corneal epithelium with Aspergillus fumigatus infection
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Author：
Li-Ting Hu, Zhao-Dong Du, Gui-Qiu Zhao, Sheng Qiu,
IF：
0.1190
Publish_to：
International Journal of Ophthalmology
PMID：
25938031
技术问答
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请问该裂解液是否可用于质谱级样品的细胞裂解?2023-07-05 14:49:53
您好，不建议用该产品裂解细胞获得蛋白后，去做质谱检测，该裂解液中的成分可能会影响质谱检测。
非变性裂解液裂解细菌菌体需要多长时间？需要超声破碎吗?2022-12-19 09:11:29
您好，需要超声破碎菌壁或者溶菌酶溶解菌壁等辅助方式，超声时间和超声功率有关，请根据实际情况摸索合适的超声时间。